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Polyvalent culture medium for anaerobic bacteria under aerobic conditions

A technology of anaerobic bacteria and aerobic bacteria, applied in the direction of bacteria, etc., can solve the problems of anaerobic bacteria growth and other issues

Pending Publication Date: 2020-06-26
FOND MEDITERRANEE INFECTION +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, media for anaerobic bacteria supplemented with this mixture of antioxidant compounds failed to grow the hard-to-grow anaerobic bacteria described above

Method used

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  • Polyvalent culture medium for anaerobic bacteria under aerobic conditions
  • Polyvalent culture medium for anaerobic bacteria under aerobic conditions
  • Polyvalent culture medium for anaerobic bacteria under aerobic conditions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] The inventors tested different antioxidants and different mixtures in the medium formulation according to the invention and selected the above mixture.

[0097] In order to select the best antioxidant mixture, the inventors tested a total of 13 antioxidants.

[0098] First, the inventors tested a base mixture of known antioxidants, namely, sodium hydrosulfide (Na 2 S), L-cysteine, ascorbic acid and glutathione, and peroxidase. Then, the same mixture was tested with superoxide dismutase and finally the mixture with oxidase. However, the inventors did not observe any improvement in the growth of anaerobic bacteria under aerobic conditions.

[0099] They then tested 6 other antioxidants with the above base mix. To do this, they experimented with 58 different combinations of all these antioxidants (see Tables 2A to 2C below).

[0100] The inventors tested these in liquid media in 24-well culture plates (Greiner Bio-One International, Kremsmunster, Austria) using several...

Embodiment 2

[0128] In order to demonstrate that this better growth of the tested anaerobic bacteria under aerobic conditions was only due to the antioxidant mixture and not the basal medium used, the inventors tested the commercially available medium Versatrek TM (I2a, Montpellier, France) in the old version of the antioxidant (sodium hydrosulfide (Na 2 S), L-cysteine, ascorbic acid, glutathione) and a new version of the antioxidant (sodium hydrosulfide (Na 2 S), L-cysteine, ascorbic acid, glutathione, catalase, panthenol and lipoic acid).

[0129] Versatrek on prior art TM Medium + 4 Antioxidants, VersaTREK to Supplement TM In the bottle (Montpellier, France I2a), supplement the following conventional antioxidant compounds with the following quantity and weight ratio in every 25mL:

[0130] – Sodium Hydrosulfide (Na 2S): 0.25g (0.025%);

[0131] – Uric acid: 0.2g (0.02%);

[0132] – Ascorbic acid: 0.5 g (0.05%);

[0133] - Glutathione: 0.05 g (0.005%).

[0134] Regarding Versatre...

Embodiment 3

[0144] Example 3: A comparative test for the growth of common strict anaerobic bacteria.

[0145] The growth performance of different media in cultures incubated in an incubator at 37 °C was evaluated below, namely, the universal flask medium according to the invention and known comparative media comprising conventional aerobic and anaerobic based on Medium BDBACTEC TM : Trypsin-enriched soybean broth ( figure 1 Medium A) and VersaTREK supplemented with the following antioxidants TM Blood culture bottle medium ( figure 1 Medium B) in (Item No. 191401, VersaTREK REDOX 1 Aerobic, from Montpellier, France I2a) [3].

[0146] The culture medium called "R-medium" according to the present invention ( figure 1 The medium C) in contains the following ingredients in the following quantity and weight ratio per 1L:

[0147] Base medium:

[0148] – casein hydrolyzate: 15 g (1.5%);

[0149] – peptone: 15g (1.5%);

[0150] – yeast extract: 10 g (1%);

[0151] – α-ketoglutarate: 2 ...

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Abstract

The present invention relates to a polyvalent culture medium for anaerobic bacteria under aerobic conditions in blood culture flasks. The present invention provides a polyvalent culture medium for culture, in an aerobic atmosphere, of anaerobic bacteria or aerobic bacteria, comprising a base culture medium for bacteria, characterized in that it also comprises the mixture of the following antioxidant compounds: sodium hydrosulfide (Na2S), L-cysteine, ascorbic acid, glutathione, catalase, ubiquinol and lipoic acid.

Description

technical field [0001] The present invention relates to a polyvalent medium for anaerobic bacteria under aerobic conditions in blood culture bottles. Background technique [0002] Anaerobic bacteria are bacteria that are sensitive to oxygen. There are strictly anaerobic bacteria (extremely sensitive to oxygen) which are developed in order to grow in absolutely no contact with oxygen, and aerotolerant anaerobic bacteria which can be exposed to low concentrations of oxygen without inhibiting their growth. Sensitivity to oxygen is linked to the fact that these bacteria lack enzyme systems. This enzyme system normally detoxifies reactive oxygen species; lack of this detoxification results in the toxic effects of oxygen on these bacteria. To allow the growth of these strictly anaerobic or oxygen-tolerant anaerobic bacteria, "anaerobic" culture methods have been implemented [1]. [0003] Reducing the time to diagnosis of bacteremia remains a major challenge for microbiologists....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor D·拉乌尔特S·赫拉菲亚M·邦纳
Owner FOND MEDITERRANEE INFECTION
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