Method for detecting fungi in blood culture negative bottle

A detection method and blood culture technology, applied in the field of medical microbiological testing, can solve problems such as difficulties in diagnosis and treatment, affecting the lives of patients, and low pass rates

Inactive Publication Date: 2018-02-16
张贵堂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The specimens sent for clinical testing have less sterile body fluids, more pollution and low pass rate, various microscopic examination methods, inconsistent standards, easy elution of fungal spores during the staining process, low microscopic examination positive rate, high culture requirements, and ordinary laboratories cann

Method used

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  • Method for detecting fungi in blood culture negative bottle
  • Method for detecting fungi in blood culture negative bottle
  • Method for detecting fungi in blood culture negative bottle

Examples

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example 1

[0036] Internal medicine patient, female, 87 years old, had a small amount of smoking, quit for 7 years, repeated coughing and wheezing for more than 8 years, aggravated for 1 year with fever for 10 days, chest CT: consistent with chronic bronchitis, bilateral lung infection, partial fibrosis, lung gas Swelling, bullae, thoracic large vessel wall, coronary artery calcification. Physical examination: P112 beats / min, heart rate 126 beats / min, coarse breath sounds in both lungs, dry and wet rales can be heard, arrhythmia, heart sounds vary in intensity. Blood test: WBC14.4×10^9 / L, RBC4.39×10^12 / L, HB107g / L, PLT364×10^9 / L, ESR115mm / H, CRP118.41mg / L, hsCRP>5.0mg / L L, sputum culture for Pseudomonas aeruginosa, blood culture was negative. Diagnosis of interstitial pneumonia, pulmonary infection, atrial fibrillation. Diagnosis and treatment: antiasthma, anti-inflammatory, phlegm-resolving treatment. After 13 days in the hospital, the discharge situation: the patient's cough, sputum...

example 2

[0038] A surgical patient, male, 67 years old, was admitted to the hospital with left foot redness, pain, fever, and left foot numbness with no obvious cause half a month ago. Medical history: "stomach disease" for more than 30 years, "dermatitis" of both lower limbs for more than 20 years, no regular treatment , repeated attacks; 5 years ago, surgery was performed for fracture of the left tibia. Diagnosis of left foot infection, eczematous dermatitis of both lower extremities, left internal tree fracture internal fixation. Auxiliary inspection not checked. Blood test: normal blood routine, CRP3.69mg / L, hsCRP5.0mg / L, ESR21mm / H. After anticoagulation and anti-infection treatment, the patient was discharged in good general condition, without fever, the redness and tenderness of the left foot dorsum were significantly relieved, and the peripheral blood supply was normal. Wet slide microscopy image 3 , long hyphae can be seen in the upper middle, and hairy cells increase. Gra...

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Abstract

A blood culture negative bottle fungus detection method is characterized in that: comprising the following steps: the first step, take the blood culture bottle stopper to be tested through alcohol lamp flame disinfection, wet film pressing and Gram stained glass slides need to be simultaneously Alcohol lamp flame disinfection, the second step, use a 5ml syringe to extract the culture in the blood culture negative bottle, first add 5 drops to each culture medium, then drop 1 drop to the glass slide to prepare wet slides, and then drop 1 drop Add it to another glass slide and spread it evenly to 1cm2 with a needle tip to prepare for Gram staining; in the third step, the cover slip is sterilized by an alcohol lamp, cooled for half a minute and then covered, then streaked and inoculated, and then microscopically inspected for wet sheet pressing , and finally stained for microscopic examination.

Description

technical field [0001] The invention relates to the field of medical microbiology inspection, in particular to a method for detecting fungus in negative blood culture bottles 2. Background technology [0002] Fungi can be isolated from the skin, oral cavity, respiratory tract, intestinal tract, vagina, etc. of normal people, and most of them are Candida, which belong to normal flora or conditional pathogenic bacteria. Fungi exist, grow, and multiply in some of our oral cavity, respiratory tract, and nasopharyngeal mucosa, but they do not cause our diseases. We all call it colonization of fungi. When our body's immune function declines or our mucosal barrier is destroyed or there is an imbalance of flora, fungi take advantage of the situation to enter our blood and enter our tissues to cause infection. Colonization of blood by fungi is not mentioned in the literature. Mycosis can be divided into superficial mycosis and invasive mycosis according to the different parts of fu...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
CPCC12Q1/04
Inventor 张贵堂
Owner 张贵堂
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