Method for detecting fungi in blood culture negative bottle

Abstract

A blood culture negative bottle fungus detection method is characterized in that: comprising the following steps: the first step, take the blood culture bottle stopper to be tested through alcohol lamp flame disinfection, wet film pressing and Gram stained glass slides need to be simultaneously Alcohol lamp flame disinfection, the second step, use a 5ml syringe to extract the culture in the blood culture negative bottle, first add 5 drops to each culture medium, then drop 1 drop to the glass slide to prepare wet slides, and then drop 1 drop Add it to another glass slide and spread it evenly to 1cm2 with a needle tip to prepare for Gram staining; in the third step, the cover slip is sterilized by an alcohol lamp, cooled for half a minute and then covered, then streaked and inoculated, and then microscopically inspected for wet sheet pressing , and finally stained for microscopic examination.

Description

technical field [0001] The invention relates to the field of medical microbiology inspection, in particular to a method for detecting fungus in negative blood culture bottles 2. Background technology [0002] Fungi can be isolated from the skin, oral cavity, respiratory tract, intestinal tract, vagina, etc. of normal people, and most of them are Candida, which belong to normal flora or conditional pathogenic bacteria. Fungi exist, grow, and multiply in some of our oral cavity, respiratory tract, and nasopharyngeal mucosa, but they do not cause our diseases. We all call it colonization of fungi. When our body's immune function declines or our mucosal barrier is destroyed or there is an imbalance of flora, fungi take advantage of the situation to enter our blood and enter our tissues to cause infection. Colonization of blood by fungi is not mentioned in the literature. Mycosis can be divided into superficial mycosis and invasive mycosis according to the different parts of fu...

AI Technical Summary

Problems solved by technology

The specimens sent for clinical testing have less sterile body fluids, more pollution and low pass rate, various microscopic examination methods, inconsistent standards, easy elution of fungal spores during the staining process, low microscopic examination positive rate, high culture requirements, and ordinary laboratories cannot The completion of fungal culture results in an increase in the false negative rate of fungal test results, which brings many difficulties to diagnosis and treatment. This invasive fungal disease is easily misdiagnosed as other intractable diseases, delaying the treatment of patients, and even affecting the lives of patients.

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