Preparation method of refined yolk antibody against duck tembusu virus

A duck Tembusu virus, egg yolk antibody technology, applied in the direction of antibodies, antiviral agents, antibody medical ingredients, etc., can solve the problem of poor and high curative effect, more than 15 days to restore feed intake, poor recovery of egg production rate, etc. question

Inactive Publication Date: 2016-06-22
CHONGQING SANJIE ZHONGXIN BIOLOGICAL ENG CO LTD
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Problems solved by technology

The virus is pathogenic to ducks of various strains. The earliest onset age of ducklings is 7 days old, 20-40 days old and high in laying period. 5 to 15%, the death rate is higher when secondary bacterial infection occurs, and it takes more than 15 d...

Method used

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Embodiment Construction

[0030] The technical solutions in the embodiments of the present invention are clearly and completely described below. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without making creative efforts belong to the protection scope of the present invention.

[0031] The virus used for the antigen of the present invention belongs to the Entaya virus group of the Mosquito-borne Virus of the Flaviviridae family Flaviviridae, has the closest relationship with Tambusu virus, and is tentatively designated as an isolate of the virus, so the disease is also called Duck Tan Busu virus disease. Source: Diagnosis and treatment of a case of duck yolk virus disease in China Poultry, Issue 02, 2012. Authors: Tang Weiguo; Shi Shaohua; Fu Guanghua; Wei Qipeng; Liu Linxiu; Ji Huayuan; Lin Jiansheng; Huang Yu.

[0032] The preparation method of duck Tembusu virus refined egg yolk antibody of this embodiment comprises the following ste...

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Abstract

The invention discloses a preparation method of a refined yolk antibody against duck tembusu virus. The preparation method comprises the following steps: antigen preparation; preparation of a duck tembusu virus transfer factor-interleukin 2 adjuvant inactivated vaccine; production of a high-immunity egg; disinfection; yolk separation; antibody extraction; and filtering to obtain a finished product. In the yolk antibody prepared by the preparation method disclosed by the invention, when the neutralizing antibody titer in the duck tembusu virus yolk is greater than or equal to 1:512, the ducks are medicated once; 2 days later, the death can be controlled, and 3 days later, normal feed intake can be recovered, and clinical disease symptoms disappear; in combination of antibiotics, the effect of controlling secondary infection of bacteria is better; the prognosis development of commercial ducklings is sound, and the economic value is not influenced; with timely treatment of commercial laying ducks and breeding ducks, the laying rate is improved by 5-13% on average after the feed intake recovers to normal for 7 days; and the refined yolk antibody against duck tembusu virus provides an effective means for clinical treatment of duck tembusu virus.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a method for preparing duck Tembusu virus refined yolk antibody. Background technique [0002] Since 2010, ducks in some parts of the country have been popular in some areas. The clinical symptoms are numb feet, decreased feed intake, loose yellow-green stools, a sudden drop in egg production, and neurological symptoms. , mild intestinal bleeding and hemorrhagic oophoritis, etc., the pathogen identified as duck Tembusu virus (clinically known as flavivirus), belongs to Flaviviridae Flavivirus genus mosquito-borne virus Entaya virus group, is Single-stranded positive-sense RNA virus. The virus is pathogenic to ducks of various strains. The earliest onset age of ducklings is 7 days old, 20-40 days old and high in laying period. 5 to 15%, the death rate is higher when secondary bacterial infection occurs, and it takes more than 15 days to restore feed intake. Commercial ducks and...

Claims

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Application Information

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IPC IPC(8): C07K16/10C07K16/02A61K39/42A61P31/14
CPCC07K16/1081A61K2039/505A61K2039/541A61K2039/545C07K16/02C07K2317/11
Inventor 魏思远赵光伟
Owner CHONGQING SANJIE ZHONGXIN BIOLOGICAL ENG CO LTD
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