Preparation method of refined yolk antibody against duck tembusu virus

Abstract

The invention discloses a preparation method of a refined yolk antibody against duck tembusu virus. The preparation method comprises the following steps: antigen preparation; preparation of a duck tembusu virus transfer factor-interleukin 2 adjuvant inactivated vaccine; production of a high-immunity egg; disinfection; yolk separation; antibody extraction; and filtering to obtain a finished product. In the yolk antibody prepared by the preparation method disclosed by the invention, when the neutralizing antibody titer in the duck tembusu virus yolk is greater than or equal to 1:512, the ducks are medicated once; 2 days later, the death can be controlled, and 3 days later, normal feed intake can be recovered, and clinical disease symptoms disappear; in combination of antibiotics, the effect of controlling secondary infection of bacteria is better; the prognosis development of commercial ducklings is sound, and the economic value is not influenced; with timely treatment of commercial laying ducks and breeding ducks, the laying rate is improved by 5-13% on average after the feed intake recovers to normal for 7 days; and the refined yolk antibody against duck tembusu virus provides an effective means for clinical treatment of duck tembusu virus.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a method for preparing duck Tembusu virus refined yolk antibody. Background technique [0002] Since 2010, ducks in some parts of the country have been popular in some areas. The clinical symptoms are numb feet, decreased feed intake, loose yellow-green stools, a sudden drop in egg production, and neurological symptoms. , mild intestinal bleeding and hemorrhagic oophoritis, etc., the pathogen identified as duck Tembusu virus (clinically known as flavivirus), belongs to Flaviviridae Flavivirus genus mosquito-borne virus Entaya virus group, is Single-stranded positive-sense RNA virus. The virus is pathogenic to ducks of various strains. The earliest onset age of ducklings is 7 days old, 20-40 days old and high in laying period. 5 to 15%, the death rate is higher when secondary bacterial infection occurs, and it takes more than 15 days to restore feed intake. Commercial ducks and...

AI Technical Summary

Problems solved by technology

The virus is pathogenic to ducks of various strains. The earliest onset age of ducklings is 7 days old, 20-40 days old and high in laying period. 5 to 15%, the death rate is higher when secondary bacterial infection occurs, and it takes more than 15 days to restore feed intake. Commercial ducks and laying ducks are stunted after recovery and the recovery of egg production rate is poor. There is currently no vaccine for prevention of this disease , causing huge economic losses to the duck farming industry