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Preparation method and application of insoluble and soluble squid melanin

A technology of squid ink and melanin, which is applied to skin care preparations, chemical instruments and methods, pharmaceutical formulations, etc., can solve the problems of time-consuming, low efficiency, unsuitable for large-scale industrial production, etc., and achieves high extraction rate and purity. high effect

Inactive Publication Date: 2016-07-06
FISHERIES RES INST OF FUJIAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation process of insoluble squid ink melanin is disclosed in "Preparation of Soluble Squid Ink Melanin and Its Antioxidative Properties by Ultrasonic Degradation Method" in Volume 37 of the Journal of Fisheries Science in 2013: "The squid ink sac is thawed at room temperature, and the epidermal membrane and internal network are removed. To obtain squid ink, add 2 times the volume of ice water and stir overnight, wash the precipitate with 20 times the volume of water at 0-4°C, centrifuge and discard the supernatant to keep the precipitate, repeat 4-6 times until the supernatant is clear and transparent, and remove the precipitate Obtain the melanin sample after vacuum-drying the object”, this method has the following defects: 1, manually remove the epidermal membrane and the internal reticular membrane of the squid ink sac, it takes a lot of time, the efficiency is low, and it is not suitable for industrialized production in large quantities; 2, The precipitate obtained by pure centrifugation contains impurities such as polysaccharides and proteins, and the obtained squid ink melanin has a low purity

Method used

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  • Preparation method and application of insoluble and soluble squid melanin
  • Preparation method and application of insoluble and soluble squid melanin
  • Preparation method and application of insoluble and soluble squid melanin

Examples

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Embodiment 1

[0032] 1. Preparation process

[0033] This example discloses a method for preparing insoluble squid ink melanin, and the specific steps and descriptions are described in detail below.

[0034] S1. Frozen and pulverized, the by-product ink sac generated during squid processing was frozen to a central temperature of -18°C, pulverized with a pulverizer, and soaked in 5 times the volume of water to thaw. In this step, the ink sac pulverized by the pulverizer contains epidermal membrane, internal reticular membrane, impurities and squid ink melanin to be extracted.

[0035] S2. Centrifuge to remove the shell, put the crushed and soaked ink sac in step S1 into a centrifuge, set the speed of the centrifuge to 300r / min-600r / min, centrifuge for 10min, discard the precipitate after centrifugation and take the supernatant. In this step, set low-speed centrifugation to centrifuge the denser epidermal membrane and internal reticular membrane contained in the crushed ink sac into the sedi...

Embodiment 2

[0078] This example discloses a preparation method of soluble squid ink melanin, and the specific steps are described in detail as follows.

[0079] Steps S1-S5 are the same as in Example 1 to obtain insoluble high-purity squid ink melanin.

[0080] S6. Adding the obtained insoluble squid ink melanin to 1-2 mol / L NaOH solution to dissolve it, and perform ultrasonic treatment;

[0081] S7. After ultrasonic treatment, add HCl solution to the solution to adjust the pH to 7.0, then centrifuge at a speed of 8000r / min to 12000r / min for 15min, discard the precipitate and retain the supernatant;

[0082] S8. After the supernatant obtained in step S7 is dialyzed for desalination, vacuum freeze-drying is performed to obtain soluble squid ink melanin.

[0083] The purity of the soluble squid ink melanin obtained in this embodiment is high. The squid ink melanin that the present embodiment obtains and the squid ink melanin standard item (BR) of Sigma-Aldrich Company carry out infrared s...

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Abstract

The invention discloses a preparation method of insoluble and soluble squid melanin.The method includes the steps of refrigerating and smashing, centrifuging and shelling, secondary centrifuging, alkali dissolving, acid depositing and the like.Prepared squid ink is high in purity, high in extracting rate and suitable for industrial large-scale production and preparation.The invention further discloses application of soluble squid melanin prepared through the method in anti-radiation skincare products.

Description

technical field [0001] The invention relates to the technical field of aquatic product processing, in particular to a preparation method and application of insoluble and soluble squid ink melanin. Background technique [0002] The scientific name of squid is squid, which is the general designation of the mollusk phylum cephalopod, lanceolate order, squid family. Squid is very popular because of its delicious taste, high protein, low fat, and rich in essential amino acids. It has the reputation of "poor man's abalone". The processing of squid mainly utilizes its carcass, and most of the by-products produced during the processing are discarded or processed into low-value feed, resulting in a great waste of resources and environmental pollution. Ink sacs accounted for about 1.3% of squid body weight in squid processing by-products. The ink sac of squid has a membrane shell on the surface and ink inside (referred to as squid ink). Squid ink is a high-density suspension compos...

Claims

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Application Information

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IPC IPC(8): C09B61/00A61K8/98A61Q17/04
CPCA61K8/987A61Q17/04C09B61/00
Inventor 刘智禹刘淑集吴靖娜苏永昌蔡水淋苏捷
Owner FISHERIES RES INST OF FUJIAN
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