Application of Chinese torreya essential oil to preparation of anti-dermatophytes cosmetics and medicine
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A technology for dermatophytes and cosmetics, applied in the field of natural medicinal chemistry, can solve problems such as the application of essential oil of Torreya torreya
Active Publication Date: 2016-07-20
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI +1
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Problems solved by technology
In the prior art, there is no application of Torreya essential oil in anti-dermatophytes
Method used
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Embodiment 1
[0020] Extraction of fresh Torreya aril essential oil by distillation and cold pressing:
[0021] Using the aril of fresh Torreya grandis (Torrey agrandiscv Meorillii) fruit as raw material, it is the green torreya skin that has been stored at room temperature for no more than 1 day. Extracting essential oil from the fresh Torreya aril can be carried out by first cutting the skin into small pieces and then adding 2 to 3 times the water. The method of distilling in water for 2 to 4 hours, or using a screw cold press to squeeze the juice, after the obtained juice is centrifuged and precipitated by a centrifuge (the centrifuge speed is 5000 rpm, and the time is 20 minutes), the supernatant is taken for distillation, Then the distillate is separated by an oil-water separator to obtain the essential oil of the fresh Torreya aril. The oil yield of Torreya essential oil obtained from fresh Torreya arils is 0.35-0.45% (w / w, wet basis weight).
[0022] CO2 supercritical extraction of ...
Embodiment 2
[0030] The present invention adopts Torreya essential oil as the active ingredient of the anti-dermatophyte product, in order to confirm the anti-dermatophyte effect of Torreya essential oil described in the present invention, the present invention carries out the anti-dermatophyte essential oil obtained from the distillation of Torreya aril. The research on ringworm activity, the specific methods and results are as follows:
[0031] 1. Experimental strains
[0032] The strains used in the in vitro antibacterial activity research of Torreya essential oil are mainly aimed at 6 common pathogenic dermatophytes that cause tinea capitis, tinea corporis and a few onychomycosis: Microsporum canis, Microsporum gypsum, Microsporum ferruginosa , Ezer floor Trichophyton, Trichophyton mentagrophytes, Epidermophyton flocculus.
[0033] 2. Experimental method
[0034] The test of the minimum inhibitory concentration MIC was carried out on a 96-microwell plate after slightly modifying the ...
Embodiment 3
[0041]
[0042] Preparation method: Add one ingredient at a time to an appropriate volume of double-distilled water with stirring until completely decomposed, and then add another ingredient. After making up to 2 ml with weighted distilled water, the solution was filtered on a sterile filter, bottled and divided according to the appropriate doses.
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Abstract
The invention belongs to the field of natural medicine and cosmetics, and provides application of Chinese torreya essential oil to preparation of anti-dermatophytes medicine, or application of Chinese torreya essential oil as efficiency ingredients in cosmetics for inhibiting the dermatophytes. In-vitro antibacterial activity study on the Chinese torreya essential oil shows that the Chinese torreya essential oil has better antibacterial activity on common pathogenic dermatophytes capable of causing tinea capitis, tinea corporis and little onychomycosis. The Chinese torreya essential oil is essential oil prepared from Chinese torreya aril by a distillation method, a cold squeezing method, a solvent extracting method or a carbon dioxide supercritical extraction method and the like. The Chinese torreya essential oil has the quality requirements that the Chinese torreya essential oil is colourless to yellowish Green liquid, and has the characteristic flavor of Chinese torreya fruit; the relative density (20 DEG C / 20 DEG C) is 0.850 to 0.860; the content sum of characteristic components of Alpha-pinene and limonene is greater than or equal to 30 percent. The invention discloses a new purpose of the Chinese torreya essential oil to prepare anti-dermatophytes medicine or cosmetics. In addition, an optimized preparation method of the Chinese torreya essential oil is provided by comparing the advantages and disadvantages of different extraction methods of the Chinese torreya essential oil.
Description
Technical field: [0001] The invention relates to the fields of natural medicinal chemistry and cosmetics, in particular to the application of Torreya torreya essential oil extracted from Torreya torreya aril as a raw material in the preparation of anti-dermatophyte medicine and cosmetics. Background technique: [0002] Torrey agrandis (Torrey agrandis), also known as Torreya grandis, Torreya grandis, etc., is an evergreen tree belonging to the family Taxaceae (Taxaceae). Zhejiang, Jiangsu, Anhui, Fujian and other provinces, the main production areas are Zhuji, Shengzhou, Shaoxing, Dongyang, Pan'an and other places in the Kuaiji Mountain area of Zhejiang Province. Torreya is a multi-purpose precious tree species that integrates food, medicine, oil, wood and ornamental greening. The fruit of Torreya torreya has been favored and affirmed all the time, but the thicker fleshy aril part of the outer layer of the seed, which accounts for 50-60% of the fresh weight of the seed, h...
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