Method for measuring relative content of puccinia striiformis DNA (deoxyribose nucleic acid) in wheat leaves

A technology of wheat stripe rust and relative content, applied in the direction of measuring devices, biochemical equipment and methods, microbial determination/inspection, etc.

Active Publication Date: 2016-07-27
CHINA AGRI UNIV
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Problems solved by technology

[0006] Aiming at the defects existing in the prior art, the present invention proposes a method for measuring the relative DNA content of stripe rust in wheat leaves to solve the problem in the prior art that the early quantitative detection of wheat stripe rust cannot be carried out simply, conveniently, accurately and quickly

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  • Method for measuring relative content of puccinia striiformis DNA (deoxyribose nucleic acid) in wheat leaves
  • Method for measuring relative content of puccinia striiformis DNA (deoxyribose nucleic acid) in wheat leaves
  • Method for measuring relative content of puccinia striiformis DNA (deoxyribose nucleic acid) in wheat leaves

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[0043] The following will clearly and completely describe the technical solutions in the embodiments of the present disclosure with reference to the accompanying drawings in the embodiments of the present disclosure. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments in the present disclosure, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present disclosure.

[0044] Such as figure 1 As shown, an embodiment of the present disclosure provides a method for measuring the relative content of stripe rust DNA in wheat leaves, the method comprising the following steps S0 to S2:

[0045] S0, building a measurement model;

[0046] S1. Obtain the near-infrared spectrum information of the leaves in the incubation period of wheat stripe rust to be tested;

[0047] S2. According to the near-infrared spectrum ...

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Abstract

The invention belongs to the technical field of plant protection and provides a method for measuring the relative content of puccinia striiformis DNA (deoxyribose nucleic acid) in wheat leaves. The method comprises steps as follows: acquiring near-infrared spectrum information of to-be-tested leaves in the incubation period of wheat stripe rust; applying a measurement model to determine the relative content of puccinia striiformis DNA corresponding to the near-infrared spectrum information of the to-be-tested leaves in the incubation period of wheat stripe rust, wherein the measurement model indicates the corresponding relation between the near-infrared spectrum information and the relative content of puccinia striiformis DNA. The method for measuring the relative content of puccinia striiformis DNA in the wheat leaves can be used for measuring the relative content of puccinia striiformis DNA in the incubation period of wheat stripe rust quickly, accurately and quantitatively.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to a method for measuring the relative DNA content of stripe rust bacteria in wheat leaves. Background technique [0002] Wheat stripe rust, caused by Puccinia striiformis f.sp.tritici (Pst), is one of the major diseases of wheat worldwide. The disease can cause severe wheat yield loss. Once an outbreak occurs, the wheat yield will be reduced by at least 10% to 30%, or even extinction. It is a major threat to the safety of wheat production in my country. Since the 1950s, wheat stripe rust has repeatedly occurred in my country, seriously affecting wheat production. In 1950, 1964, 1990 and 2002, my country's wheat production was reduced by 60, 30, 2.6 and 1.4 billion kg, respectively. An infection process of wheat stripe rust in the field generally includes four periods: contact period, invasion period, incubation period and disease onset period. The wheat stripe rust that ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/359
CPCC12Q1/6851G01N21/359C12Q2545/113C12Q2537/143C12Q2537/165
Inventor 王海光赵雅琼谷医林秦丰温丽刘彬彬马占鸿赵龙莲李军会李小龙程培潘阳
Owner CHINA AGRI UNIV
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