A method for preparing nano-red element selenium by endophytic Helix spirulina of tea tree

A technology for Ophispira spirochetes and tea tree, which is applied in the field of producing nano-red element selenium by biological cells, can solve the problems of not involving the application of endophytic Oxyspira spirochetes in tea trees, etc., and achieves the effects of low cost, convenient operation and high conversion rate

Inactive Publication Date: 2020-11-06
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The literature "Microbiological characteristics of two tea tree endophytic Helicospirosis" (Wang Ting et al., Journal of Microbiology 2014 No. 4, 2014.04.04, P424-432) described in detail the screening and microbiological characteristics of tea tree endophytic Helicospirosis , but did not involve the application of endophytic Helicospira in tea trees

Method used

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  • A method for preparing nano-red element selenium by endophytic Helix spirulina of tea tree
  • A method for preparing nano-red element selenium by endophytic Helix spirulina of tea tree
  • A method for preparing nano-red element selenium by endophytic Helix spirulina of tea tree

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Experimental program
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Effect test

Embodiment 1

[0022] (1) Inoculate 5 mL of nutrient broth medium (10 g of tryptone, 3 g of ground beef, 5 g of NaCl, 10 μg / mL of spectinomycin, 5 μg / mL of ampicillin, and Add water to 1000ml), shake at 150 rpm, and incubate overnight at 28°C.

[0023] (2) Add the activated bacterial solution to fresh 50-200mM mMNa at a volume ratio of 1:100 2 SeO 4 LB liquid medium (10g tryptone, 5g yeast extract, 10g NaCl, 10μg / mL spectinomycin, 5μg / mL ampicillin, 50-200mM Na 2 SeO 4 , add water to 1000ml, pH7.2-7.4) to scale up and cultivate, the culture conditions are: the shaker speed is 150 revolutions per minute, and the temperature is 37°C. After culturing for 6-8 hours, the culture medium began to turn red, indicating that red anhydrous selenium had been produced, and then with the prolongation of culture time, the red color gradually deepened.

[0024] (3) Morphological detection of red non-selenium nanoparticles: take 100 μl of bacterial culture solution in step (3), mix with 900 μl of phospha...

Embodiment 2

[0026] (1) Put the endophytic Helicospirilla strains stored at -80°C directly into 5ml LB liquid medium containing selenate (10g tryptone, 5g yeast extract, 10g NaCl, 10μg / mL spectinomycin, 5 μg / mL ampicillin, 50 mM Na 2 SeO 4 , add water to 1000ml, pH7.2-7.4), shake at 150 rpm, and incubate overnight at 28°C.

[0027] (2) Add the activated bacterial solution to fresh 200mM Na at a volume ratio of 1:100 2 SeO 4 LB liquid medium (10g tryptone, 5g yeast extract, 10g NaCl, 10μg / mL spectinomycin, 5μg / mL ampicillin, 200mM Na 2 SeO 4 , add water to 1000ml, pH7.2-7.4) to enlarge the culture. The culture conditions are as follows: the rotation speed of the shaker is 150 rpm, and the temperature is 37°C. After culturing for 6-8 hours, the culture medium began to turn red, indicating that red anhydrous selenium had been produced, and then with the prolongation of culture time, the red color gradually deepened.

[0028](3) Morphological detection of red non-selenium nanoparticles:...

Embodiment 3

[0030] (1) Put the endophytic Helicospirilla strains stored at -80°C directly into 5ml LB liquid medium containing selenate (10g tryptone, 5g yeast extract, 10g NaCl, 10μg / mL spectinomycin, 5 μg / mL ampicillin, 50 mM Na 2 SeO 4 , add water to 1000ml, pH7.2-7.4), shake at 150 rpm, and incubate overnight at 28°C.

[0031] (2) Directly insert the activated bacteria into fresh solution containing 200mM Na at a volume ratio of 1:100 2 SeO 4 LB liquid medium (10g tryptone, 5g yeast extract, 10g NaCl, 10μg / mL spectinomycin, 5μg / mL ampicillin, 200mM NaCl 2 SeO 4 , add water to 1000ml, pH7.2-7.4) to enlarge the culture. The culture conditions are as follows: the rotation speed of the shaker is 150 rpm, and the temperature is 37°C. After incubating for 5 hours, add 200mM Na 2 SeO 4 , so that the total concentration of sodium selenate was 400 mM. Continue culturing for 8-10 hours under the conditions of a shaker with a rotating speed of 150 rpm and a temperature of 37°C.

[0032...

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Abstract

The invention relates to a method for preparing nano red elemental selenium through endophytic bacteria isolated from tea (Camellia sinensis L.) .Through the biological characteristic that endophytic bacteria isolated from tea (Camellia sinensis L.) has strong reduction activity on selenate, selenate and endophytic bacteria isolated from tea (Camellia sinensis L.) are directly cultured together, toxic selenate is subjected to reduction to obtain non-toxic red elemental selenium, and high-purity red elemental selenium nanospheres or nanoflowers can be obtained through the control over culture time .The technological method is low in cost, easy to operate, high in conversion efficiency, safe and environmentally friendly .

Description

technical field [0001] The invention relates to a technology for producing nano-red element selenium by biological cells, in particular to a method for converting toxic selenate and crystallizing into non-toxic nano-red element selenium by using endophytic Helix spirochete in tea trees. Background technique [0002] Selenium is one of the essential trace elements for humans and animals, and has an important relationship with the body's antioxidant capacity, immune function, anti-virus, and anti-cancer effects. Compared with inorganic selenium and organic selenium, red nano-selenium (zero-valent selenium) has the characteristics of low toxicity and high biological activity. In view of the unique biological effects of zero-valent selenium such as low toxicity, high-efficiency anti-oxidation, anti-cancer, neutralization of heavy metal toxicity, and high absorption and utilization rate, it has broad application prospects in domestic animal production, medicine, and health produc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00C12N1/20C12R1/01
CPCC12N1/20C12P3/00
Inventor 王行国徐萧刘欣喻雪婧李亚东
Owner HUBEI UNIV
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