Bacillus licheniformis purification method

A technology of Bacillus licheniformis and a purification method, which is applied in the field of microorganisms, can solve the problems of low purity of microorganisms and affect the use, achieve the best growth performance and improve the success rate

Inactive Publication Date: 2016-08-24
SHANDONG CREATE YIFENG FERTILIZER GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is currently no reasonable method to achieve the purification of Bacillus licheniformis, resulting in a very low purity of the microorganisms, which affects subsequent use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for purifying bacillus licheniformis, comprising the steps of:

[0033] (1) Take the strain of Bacillus licheniformis, inoculate it on the slant of a fresh test tube, and incubate it at 31°C for 19 hours, then take the cultured slant, add 10m L of sterile saline, and gently scrape off the bacterial lawn on the slant with an inoculation loop , oscillating sterile saline, then pouring the sterile saline into another No. 1 triangular flask filled with 30mL of sterile saline, putting four nutrient beads in the bottle (the nutrient beads comprise the following raw materials prepared in parts by weight Preparation: tryptone 1, yeast extract powder 0.7, NaCl 1.2; pH 7.3), then shake on a shaker at a speed of 100r / min for 25min to make a uniform bacterial suspension, store at 5°C for later use;

[0034] (2) Get the first culture medium (the first culture medium comprises the raw material preparation of following parts by weight: peptone 5, beef extract 4, NaCl 5), and ...

Embodiment 2

[0049] A method for purifying bacillus licheniformis, comprising the steps of:

[0050] (1) Take the strain of Bacillus licheniformis, inoculate it on the slant of a fresh test tube, and incubate it at 33°C for 20 hours, then take the cultivated slant, add 12m L of sterile saline, and gently scrape off the bacterial lawn on the slant with an inoculation loop , oscillating sterile saline, then pouring the sterile saline into another No. 1 triangular flask filled with 34m L of sterile saline, putting four nutrient beads in the bottle (the nutrient beads include the following raw materials prepared in parts by weight Preparation: tryptone 1.3, yeast extract powder 0.8, NaCl 1.3; pH 7.3), then shake on a shaker at a speed of 120r / min for 26min to make a uniform bacterial suspension, and store it at 6°C for later use;

[0051] (2) Take the first culture medium (the first culture medium is prepared from the following raw materials by weight: peptone 6, beef extract 4.5, NaCl 6), and...

Embodiment 3

[0066] A method for purifying bacillus licheniformis, comprising the steps of:

[0067] (1) Take the strain of Bacillus licheniformis, inoculate it on the slant of a fresh test tube, incubate at 35°C for 25 hours, then take the cultured slant, add 15m L of sterile saline, and gently scrape off the bacterial lawn on the slant with an inoculation loop , oscillating sterile saline, then pouring the sterile saline into another No. 1 triangular flask filled with 35m L of sterile saline, putting four nutrient beads in the bottle (the nutrient beads comprise the following raw materials prepared in parts by weight Preparation: tryptone 1.5, yeast extract powder 0.9, NaCl 1.3; pH 7.3), then shake on a shaker at a speed of 130r / min for 27min to make a uniform bacterial suspension, and store it at 7°C for later use;

[0068] (2) Take the first culture medium (the first culture medium is prepared from the following raw materials by weight: peptone 7, beef extract 5, NaCl 7), and add the c...

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PUM

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Abstract

The invention discloses a bacillus licheniformis purification method. The method comprises the following steps: (1) taking a bacillus licheniformis strain, inoculating the bacillus licheniformis strain, preparing uniform bacterial suspension and preserving the bacterial suspension at 5-7 DEG C for later use; (2) adding a first culture medium to a No.2 conical flask and carrying out shaking culture, thus obtaining a strain; (3) taking a second culture medium, adding distilled water to the culture medium, boiling the culture medium on an electric furnace for 5-10min, cooling the second culture medium to room temperature and solidifying the second culture medium for later use; (4) melting the second culture medium, coating the second culture medium on a plate, then carrying out streak culture on the strain on the plate and separating and purifying bacillus licheniformis; and (5) cultivating separated and purified bacillus licheniformis. The method has the beneficial effects that the success rate of purification is increased by further separating and purifying the bacillus licheniformis strain sold on the market after culturing, enlarging and adapting the bacillus licheniformis strain, so that the purified bacillus licheniformis strain fully shows better growth performance.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for purifying bacillus licheniformis. Background technique [0002] Bacillus (Bacillus Cohn, 1872), the cells are straight rods, 0.5-2.5 μm × 1.2-10 μm, often arranged in pairs or chains, with round or square ends. Cell staining was mostly Gram-positive in young cultures and moved with perinatal flagella. The spores are oval, oval, columnar, and round, and can resist many adverse environments. Each cell produces a spore, and the sporulation is not inhibited by oxygen; aerobic or facultative anaerobic, with tolerance to different environments such as heat, pH and salt. It belongs to the chemoheterotrophic bacteria with fermentative or respiratory metabolism, usually positive for contact enzymes. Bacillus species are found in diverse environments and a few species are pathogenic to vertebrates and invertebrates. The Bacillus genus is a group of aerobic and facul...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12R1/10
CPCC12N1/20C12N1/02
Inventor 刘亚民
Owner SHANDONG CREATE YIFENG FERTILIZER GRP CO LTD
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