White rot fungi secretion manganese peroxidase culture medium and preparation method thereof

A technology of manganese peroxidase and medium, which is applied in the field of white rot fungus secreting manganese peroxidase medium and its preparation, can solve the problems of limited application, low manganese peroxidase production and low activity, etc. To achieve the effect of solving the problem of environmental pollution

Active Publication Date: 2016-08-24
ANQING NORMAL UNIV
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  • Abstract
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Problems solved by technology

[0003] Although manganese peroxidase has such an important use, its application in production practice is still very limited. The main reason is that the activity of manganese peroxidase synthesized by most production strains is low, resulting in low manganese peroxidase production.

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  • White rot fungi secretion manganese peroxidase culture medium and preparation method thereof
  • White rot fungi secretion manganese peroxidase culture medium and preparation method thereof
  • White rot fungi secretion manganese peroxidase culture medium and preparation method thereof

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Embodiment Construction

[0030] The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0031] 1. Determination of manganese peroxidase activity

[0032] The method for the determination of manganese peroxidase activity was 2,6-dimethoxyphenol method. 1mL reaction system containing 50μL (10mmol / L) of the reaction substrate 2,6-dimethoxyphenol (2,6-DMP), sodium malonate buffer (50mmol / L) 840μL, MnSO 4 (10mmol / L) 50μL, H 2 o 2 (10mmol / L) 10μL start reaction and 50μL diluted enzyme solution, start the reaction at 25°C and measure the increase value ΔOD470 of the absorbance of the reaction solution at 470nm within 1 min before the reaction, with the inactivated enzyme solution as the blank. Under this...

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Abstract

The invention discloses a white rot fungi secretion manganese peroxidase culture medium and a preparation method thereof. The white rot fungi secretion manganese peroxidase culture medium is low-nitrogen asparaginate-succinic acid culture medium, wherein the culture medium comprises a 5-30g / L carbon source, a 5-30mmol / L nitrogen source, 26.7-200mu mol / L manganese ions, 0.25-0.75mL / L Tween 80, 0.1-0.4g / L MgSO4.7H3O, and 10-20ml / L of a mineral element solution; the pH value of a culture liquid is 3.5-5.5. The lignin-degrading enzyme manganese peroxidase in white rot fungi is adopted for biological degradation on lignin, and the lignin can be finally and thoroughly degraded as CO2 and H2O, so that the environment pollution problem of the paper-making industry can be fundamentally solved. Manganese peroxidase can be adopted to degrade lignin and phenol substances, and various organic pollutants including long-lasting solid chemical agricultural pesticide, dye decoloring agents and the like can be attacked.

Description

technical field [0001] The invention relates to a manganese peroxidase culture medium, in particular to a manganese peroxidase culture medium secreted by white rot fungi and a preparation method thereof. Background technique [0002] Lignin is a natural macromolecular organic matter second only to cellulose in the plant kingdom, and is the most abundant aromatic compound that can be obtained from renewable resources. The biodegradation of lignin plays a key role in the earth's carbon cycle. There are many microorganisms that degrade lignin in nature. They can attack and degrade lignin and cause wood white rot. It can produce some extracellular oxidases in the lignin cell cavity, mainly including manganese peroxidases (MnPs), laccases (Laccases / Lac) and lignin peroxidases (Lignin peroxidases / LiPs), which are the main components of the lignin-degrading enzyme system of white rot fungi, among which MnP is a H-dependent 2 o 2 The heme glycoproteinase is the most common ligni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/08C12R1/645
Inventor 尹立伟杨春成吴甘霖孙廷哲穆丹宋晓贺许远朱玉张玉龙池玉杰
Owner ANQING NORMAL UNIV
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