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Bacillus licheniformis powder and preparation method thereof

A technology of bacillus licheniformis and powder, which is applied in botany equipment and methods, fungicides, biocides, etc., can solve the problems of spore loss, low spore yield, and low efficiency, and achieve the purpose of inhibiting the deterioration of fermentation broth and improving the yield of spores efficiency and cost reduction

Inactive Publication Date: 2016-08-31
SHANXI KINGSHINE FERTILIZER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The disadvantages of this process are low efficiency, high cost, deterioration of fermentation broth in the production process, serious loss of spores, and low yield of spores.
[0007] In addition, there are different dry and solid excipients. Some dry and solid excipients have high cost, and some dry and solid excipients affect product quality, and the amount of dry and solid excipients also affects product quality.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The components of a Bacillus licheniformis powder of the present invention include Bacillus licheniformis and dry solid auxiliary materials, and the Bacillus licheniformis includes three kinds of lichens with preservation numbers ACCC11080, ACCC10613 and ACCC11106 preserved in China Agricultural Microorganism Culture Collection Center Bacillus, the dry solid auxiliary materials are white carbon black and bentonite, and the content ratio of each component is: 30-40 kg of white carbon black, 40-50 kg of bentonite, and 100-110 trillion Bacillus licheniformis live.

Embodiment 2

[0025] According to the Bacillus licheniformis powder described in Example 1, the Bacillus licheniformis is one, two or three of the preservation numbers of ACCC11080, ACCC10613, and ACCC11106 preserved in the China Agricultural Microorganism Culture Collection Center. Bacillus licheniformis.

Embodiment 3

[0027] A kind of preparation method of Bacillus licheniformis powder of the present invention, take a kind of Bacillus licheniformis as example (two or three kinds of preparation methods are all the same),

[0028] (1) Take the preservation number ACCC11080 Bacillus licheniformis fermentation liquid preserved in the China Agricultural Microorganism Culture Collection Center. The number of live bacteria in this batch of fermentation liquid was detected to be 6.7 billion / g, the dry solid content was 5.3%, and the pH was 7.1; This batch of fermentation broth was heated up to 70°C, kept warm for 30 minutes, then cooled to 45°C to kill a small amount of unsporulated bacilli, and put into a tank with a volume of 21.3m³;

[0029] (2) Filtration and liquid separation: the fermented liquid after putting in the tank is filtered through a 50nm ceramic membrane, the filtered clear liquid is 16.8m³, and the remaining concentrated liquid is 4.5m³, and it takes 2 hours;

[0030] (3) Concentr...

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Abstract

The invention provides bacillus licheniformis powder and a preparation method thereof. The bacillus licheniformis are three types of bacillus licheniformis which are preserved in Agricultural Culture Collection of China and have the preservation numbers of ACCC11080, ACCC10614 and ACCC11106 respectively. One or two or three types of fermentation liquid of bacillus licheniformis are taken to be heated to 50-70 DEG C, heat is preserved for 30 min, and then the fermentation liquid is cooled to 45-55 DEG C, put in a tank, and filtered to obtain clear liquid and thick liquid; clear liquid is concentrated to be mixed with thick liquid, white carbon black and bentonite are put in to be stirred and mixed, and spray drying, cooling and screening are conducted to obtain the bacillus licheniformis powder. By means of the method, fermentation liquid deterioration caused by microorganism infection in the production process can be effectively inhibited; compared with a traditional process, the spore yield is increased by 10.97%; the concentration time is shortened, efficiency is improved by 155%, energy is saved, and cost is reduced; compared with a traditional process, cost is reduced by 50%, and cost is reduced by 60.97% in total.

Description

technical field [0001] The invention relates to a production method of bacillus licheniformis spore powder. Background technique [0002] Bacillus licheniformis (scientific name: Bacillus licheniformis) is a Gram-positive thermophilic bacterium commonly found in soil. It may exist in the form of spores to resist harsh environments; under favorable conditions, it can exist in a growing state. The bacterium can adjust the imbalance of flora to achieve the purpose of treatment, can promote the body to produce antibacterial active substances, kill pathogenic bacteria, can produce antiactive substances, and has a unique mechanism of biological oxygen capture, which can inhibit the growth and reproduction of pathogenic bacteria. It is widely used in the fields of medicine, feed and agriculture. The Bacillus licheniformis described in the present invention is respectively, three kinds of Bacillus licheniformis preserved in China Agricultural Microorganism Culture Collection Cente...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K20/28A61K35/742A61K9/14A61P31/04A01N63/00A01N25/12A01P1/00A01P3/00A01P7/00
CPCA61K35/742A01N25/12A01N63/00A61K9/143A01N2300/00
Inventor 李海军王经君胡军杨文龙黄振锋
Owner SHANXI KINGSHINE FERTILIZER
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