Bacillus licheniformis powder and preparation method thereof
A technology of bacillus licheniformis and powder, which is applied in botany equipment and methods, fungicides, biocides, etc., can solve the problems of spore loss, low spore yield, and low efficiency, and achieve the purpose of inhibiting the deterioration of fermentation broth and improving the yield of spores efficiency and cost reduction
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Embodiment 1
[0023] The components of a Bacillus licheniformis powder of the present invention include Bacillus licheniformis and dry solid auxiliary materials, and the Bacillus licheniformis includes three kinds of lichens with preservation numbers ACCC11080, ACCC10613 and ACCC11106 preserved in China Agricultural Microorganism Culture Collection Center Bacillus, the dry solid auxiliary materials are white carbon black and bentonite, and the content ratio of each component is: 30-40 kg of white carbon black, 40-50 kg of bentonite, and 100-110 trillion Bacillus licheniformis live.
Embodiment 2
[0025] According to the Bacillus licheniformis powder described in Example 1, the Bacillus licheniformis is one, two or three of the preservation numbers of ACCC11080, ACCC10613, and ACCC11106 preserved in the China Agricultural Microorganism Culture Collection Center. Bacillus licheniformis.
Embodiment 3
[0027] A kind of preparation method of Bacillus licheniformis powder of the present invention, take a kind of Bacillus licheniformis as example (two or three kinds of preparation methods are all the same),
[0028] (1) Take the preservation number ACCC11080 Bacillus licheniformis fermentation liquid preserved in the China Agricultural Microorganism Culture Collection Center. The number of live bacteria in this batch of fermentation liquid was detected to be 6.7 billion / g, the dry solid content was 5.3%, and the pH was 7.1; This batch of fermentation broth was heated up to 70°C, kept warm for 30 minutes, then cooled to 45°C to kill a small amount of unsporulated bacilli, and put into a tank with a volume of 21.3m³;
[0029] (2) Filtration and liquid separation: the fermented liquid after putting in the tank is filtered through a 50nm ceramic membrane, the filtered clear liquid is 16.8m³, and the remaining concentrated liquid is 4.5m³, and it takes 2 hours;
[0030] (3) Concentr...
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