Paper chip colorimetric analysis system for mercury ion detection and construction method and application thereof

A technology of colorimetric analysis and construction method, which is applied to the paper chip colorimetric analysis system for mercury ion detection and its construction field, can solve the problems of expensive instruments, cumbersome operation procedures, detection of mercury ions, etc., and achieves the effect of good application prospect.

Inactive Publication Date: 2016-09-28
FUDAN UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods can achieve sensitive and rapid detection of mercury ions, they often require cumbersome operating procedures and expensive instruments, so they are not suitable for real-time on-site detection of mercury ions at pollution sources.

Method used

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  • Paper chip colorimetric analysis system for mercury ion detection and construction method and application thereof
  • Paper chip colorimetric analysis system for mercury ion detection and construction method and application thereof
  • Paper chip colorimetric analysis system for mercury ion detection and construction method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1: Paper Chip Colorimetric Detection of Mercury Ion Content and Detection Limit Investigation

[0039] Schematic diagram of paper chip colorimetric detection of mercury ions figure 2 shown. The specific steps are as follows: add 2 mL of PtNPs solution diluted to a fixed multiple into a plastic dropper bottle, then add the same volume of aqueous solution containing mercury ions, shake and mix well and let stand for 2-3 min. Then assemble the paper chip element and drop 15 ul -20 ulTMB / H on the paper substrate 2 o 2 Chromogenic substrate solution, and then carefully and slowly add a drop of the mixture in the dropper bottle into the detection area. Let the paper chip rest for 5-6 minutes to ensure complete reaction, then use a digital camera or smart phone to take pictures of the paper chip to record the colorimetric results; at the same time, place it under the probe of the fiber optic device to read the chip accurately by numerical method Color intensity. ...

Embodiment 2

[0040] Example 2: Selective investigation of paper chip colorimetric detection of mercury ions

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Abstract

The invention belongs to the technical field of mercury ion detection, and in particular, relates to a paper chip colorimetric analysis system for mercury ion detection and a construction method and an application thereof. The construction of the paper chip colorimetric analysis system includes the steps of preparation of a paper chip plastic bearing substrate, and design and preparation of the paper chip; the paper chip is similar to a micro reaction vessel and is used for platinum nanoparticle catalysis of a TMB/H2O2 developing reaction; the construction of a simple optical fiber analysis device is used for representation of the intensity of a color in the paper chip in a numerical value form. A principle comprises that platinum nanoparticles have excellent activity similar to catalase and can rapidly catalyze a TMB/H2O2 substrate to generate a blue precipitate, while when mercury ions exist, PtNPs can quickly undergo a reaction with the mercury ions to inhibit self enzyme activity, and finally, the recognition and quantitation of the mercury ions in the solution are achieved through changes of the color after the reaction. The system has the advantages of excellent specificity and selectivity, and has wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of mercury ion detection, and in particular relates to a paper chip colorimetric analysis system for mercury ion detection and its construction method and application. Background technique [0002] The rapid development of industry has led to the indiscriminate discharge of industrial sewage and the continuous accumulation of human garbage, resulting in increasingly serious heavy metal pollution. Among them, mercury ions are a highly toxic pollutant that commonly exists in the external environment. Studies have shown that when mercury is ingested by humans through drinking water or biological chains, it cannot be excreted through metabolism. Therefore, due to the accumulation effect of mercury ions and the high toxicity of mercury ions, it will cause serious harm to human health. For example, the accumulation of trace amounts of mercury ions in the human body will cause brain damage, kidney failure, and cent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 陈伟伟方雪恩李花曹宏梅杨茜王丽娟孔继烈
Owner FUDAN UNIV
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