Kit for HCV virus genotyping detection and SNP locus detection of IL28B, use method and application thereof

Abstract

The invention discloses a kit for HCV virus genotyping detection and SNP locus detection of IL28B, a use method and an application thereof. The kit includes a nucleic acid amplification reagent and a hybridization reagent. The use method of the kit includes the steps of: 1) nucleic acid extraction of a sample; 2) preparation of the amplification reagent; 3) PCR amplification; 4) a hybridization reaction; 5) a fluorescent reaction; 6) detection; and 7) determination on the genotype of HCV virus and the genotype of an SNP locus in the IL28B gene. The kit can be used for detecting nine different HCV viruses and meanwhile detecting the genotypes of two loca in the IL28B gene which relates to treatment effect on the HCV. The kit has high flux, high sensitivity and high type coverage area, allows multi-target simultaneous detection and is simple in operation.

Description

Technical field [0001] The invention relates to a kit for genotyping and SNP (Single Nucleotide Polymorphism) site detection and its use method and application, in particular to a hepatitis C virus genotyping and IL28B SNP site The detection kit and its use method and application. Background technique [0002] Hepatitis C virus (HCV), first discovered in 1989, is the world's most common non-A, non-B hepatitis virus obtained from blood transfusion and community. HCV is a folded single-stranded positive sense RNA virus, which is a separate genus in the Flaviviridae. Around the world, at least six genotypes and multiple subtypes and virus variants have been found. Infection with different genotypes will affect the severity of the disease and the response to treatment. HCV is mainly transmitted through contaminated blood and blood products, and the possibility of infection through human secretions is very low. [0003] The prevalence of HCV exceeds 3.0% worldwide, and the carrier r...

AI Technical Summary

Problems solved by technology

The main reason is that the channels of the real-time fluorescent PCR method are limited, and simultaneous testing of multiple targets cannot be achieved.

Images