Cultivation method for quercus variabilis aseptic seedling

A cultivation method and technology of sterile seedlings, applied in the field of seedling cultivation, can solve problems such as cumbersome procedures and long soaking time, and achieve the effects of simple operation process, improved seedling uniformity, and reduced production costs

Inactive Publication Date: 2016-10-12
INST OF SOIL FERTILIZER SICHUAN ACAD OF AGRI SCI
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Problems solved by technology

[0003] In existing reports, the application number is CN201210289748.9 "A Cultivation Method for Quercus Cork Seedlings", there are formalin disinfectants, geographical restrictions, too long soaking time (46-52h), repeated rooting powder Handling and other cumbersome procedures

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  • Cultivation method for quercus variabilis aseptic seedling

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Embodiment 1

[0030] A method for cultivating an aseptic seedling of Quercus cork for inoculating truffles, comprising the following steps:

[0031] (1) Self-picked mature new species with a purity of 94%;

[0032] (2) After picking the seeds, perform grain sorting, remove seeds damaged by diseases and insect pests and with abnormal color, and select seeds with uniform color, full size, no mildew, no insects, and no floating;

[0033] (3) After rinsing the seeds with tap water for 3 times, disinfect them with 0.5% potassium permanganate solution for 2 hours (the ambient temperature is 30°C, it is advisable to submerge the seeds by more than 2cm in the disinfectant solution), seal the seeds with a sealed bag for 30 minutes, and then use sterile Rinse with water 5 times;

[0034] (4) Soak the seeds in sterile warm water with an initial temperature of 55°C for 4 hours, then soak the seeds in 100 mg / L gibberellin solution for 24 hours, and place the seeds in an incubator at 30°C during the soa...

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Abstract

The invention belongs to the field of seedling cultivation, and in particular relates to a method for cultivating aseptic seedlings of oak cork, especially the aseptic seedling of oak cork can be used to inoculate truffles. The object of the invention is to provide a kind of cultivation method of aseptic seedlings of Quercus variabilis, which adopts safer potassium permanganate solution disinfection, simple treatment process, saves seedling cultivation time and more remarkable seedling cultivation effect, and the Quercus variabilis that above-mentioned cultivation method obtains has no Bacterins are especially suitable for inoculating truffles. Its steps are: (1) seed selection; (2) seed disinfection treatment; (3) germination; (4) sowing; (5) emergence management; adopting this method can effectively improve the time for truffle mycorrhizal seedling formation, improve The germination rate and the seedling rate of the oak cork seeds improve the uniformity of the seedlings and significantly improve the quality of the seedlings, and the oak cork seedlings cultivated by the invention can be directly used for the inoculation of truffles.

Description

technical field [0001] The invention belongs to the field of seedling cultivation, and in particular relates to a method for cultivating aseptic seedlings of oak cork, especially the aseptic seedling of oak cork can be used to inoculate truffles. Background technique [0002] Cork oak (Quercus variabilis) is one of the most widely distributed tree species in my country. It is also an important tree species of cork and tannin extract resources, and it is symbiotic with a variety of ectomycorrhizal fungi. Under natural conditions, in the formation of secondary forests, seedlings sprouting from piles is the most important way to grow seedlings, and the completion of life history of seedlings is greatly affected by the environment and the lack of lateral fibrous roots of seedlings. The survival rate of cork oak seedling afforestation is only 30-50%. [0003] In existing reports, the application number is CN201210289748.9 "A Cultivation Method for Quercus Cork Seedlings", there ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01C1/02
Inventor 李小林叶雷陈诚彭卫红黄忠乾甘炳成郑林用
Owner INST OF SOIL FERTILIZER SICHUAN ACAD OF AGRI SCI
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