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Space microbacterium oxydans LCT-H6

A kind of LCT-H6, oxidizing microbacteria technology, applied in the field of microorganisms and biology

Inactive Publication Date: 2016-10-12
GENERAL HOSPITAL OF PLA
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Problems solved by technology

[0003] For the research on the corrosion of microbial materials in space environment, there is no relevant report on microbacteria oxidans

Method used

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Examples

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Embodiment Construction

[0022] The experimental methods used in the following implementation examples are conventional methods unless otherwise specified.

[0023] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0024] 1. Phenotype of Microbacterium oxidans LCT-H6

[0025] 1. The source of the strain: Microorganisms were collected from the condensed water inside the return cabin of the "Shenzhou 9" spacecraft, and the bacterial culture medium was selected for preliminary screening of the samples, and then single colonies with different colony shapes were picked for streak culture and purified to After a single strain, use physiological saline plus 80% glycerol to preserve the strain. The strain is named Microbacterium oxidans LCT-H6, and it is preserved in China General Microorganism Culture Collection Center with the preservation number CGMCC No.12574.

[0026] 2. Gram staining: take 50ul of the sample and centrifuge, ...

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Abstract

The invention relates to space microbacterium oxydans LCT-H6, in particular to a space microorganism which is obtained through separation of condensed water in a 'Shenzhou 9' spacecraft return capsule with a space technology. The space microbacterium oxydans LCT-H6 is characterized in that microbacterium oxydans LCT-H6 is an aerobic gram-positive bacterium, has the closest genetic relationship with microbacterium maritypicum, and the space microbacterium oxydans LCT-H6 has corrosion capability for four polymer materials including epoxy resin, ester type polyurethane, ether type polyurethane and vulcanized natural rubber. The space microbacterium oxydans LCT-H6 is clarified on the level of genomics, the total length of a whole genome sequence of LCT-H6 proves to be 4.11 Mbp, the content of GC is 67.65%, protein of the space microbacterium oxydans LCT-H6 is classified and noted as the class-22 COG family, and COG containing a largest amount of protein has functions on 'energy generation and conversion', 'amino acid transportation and metabolism', 'sugar transportation and metabolism', 'inorganic salt ion transportation and metabolism' as well as 'translation, ribosome structure and forming'.

Description

technical field [0001] The invention belongs to the field of microbes and biotechnology, and relates to a new microbial strain Microbacterium oxidans LCT-H6 and its biological characteristics and genome DNA sequence. Bioinformatics technology is used to conduct omics analysis to obtain its genome characteristics. Background technique [0002] With the increasing frequency of human space exploration activities, space has become an important field of human activities. There is a specific microbial community in the space capsule. Although every item has been strictly sterilized, microorganisms will still enter space with the astronauts' bodies, human secretions, aviation components, etc., and form a specific microbial community in the airtight cabin of the spacecraft, including both harmless microorganisms and some Pathogenic and corrosive microorganisms. During the more than ten years of Mir's operation, 234 kinds of microorganisms were detected in the space station. The sp...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/205C12R2001/01
Inventor 刘长庭张学林方向群郭英华王俊峰姜学革李天志周宏刘岩潘磊徐绸黄兵李佳余昳
Owner GENERAL HOSPITAL OF PLA
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