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Method for preparing aniline green artificial antigens

A technology of artificial antigen and artificial hapten, which is applied in the field of preparation of aniline green artificial antigen, can solve the problems of expensive equipment, time-consuming detection, and failure to achieve, and achieve the effect of advanced synthesis technology and strong specificity

Inactive Publication Date: 2016-10-26
HANGZHOU LAIHE BIOTECH CO LTD
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Problems solved by technology

[0005] At present, the detection of aniline green mainly relies on high-performance liquid chromatography (HPLC), gas chromatography (GC), thin-layer chromatography (TLC), simple (MS), etc., but there are expensive instruments, time-consuming detection, and professional and technical personnel The operation cannot meet the fast and accurate requirements of modern detection

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  • Method for preparing aniline green artificial antigens
  • Method for preparing aniline green artificial antigens
  • Method for preparing aniline green artificial antigens

Examples

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Embodiment 1

[0032] (1) Preparation of artificial hapten---synthesis of hapten 4-MGC:

[0033] (a) Add N,N-dimethylaniline (3g) and 4-carboxybenzaldehyde into a round-bottomed flask at a molar ratio of 2:1, and stir at 100°C for 6 hours; after the reaction, the reaction solution is extracted with ether , dried, and recrystallized to obtain a white solid product Ⅰ;

[0034] (b) Weigh 2g of product I, add 30ml of benzene and 5g of Amberlyst 15 resin catalyst into a round-bottomed flask, protect it under nitrogen, and stir at reflux at 130°C overnight; after the reaction, dry the solvent under reduced pressure and extract it with ether to obtain Product II;

[0035] (c) The product II was purified by silica gel column chromatography, and extracted to obtain the target hapten 4-(bis(4-dimethylamino)phenyl)methylbenzoic acid (4-MGC) as a white solid;

[0036] (2) Preparation of aniline green artificial antigen---synthesis of aniline green-BGG:

[0037] (d) Weigh 0.425g of hapten 4-MGC and 0....

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Abstract

The invention provides a method for preparing aniline green artificial antigens. The method includes steps of firstly, preparing and detecting haptens, and to be more specific, synthesizing the aniline green haptens from N, N-dimethylaniline and benzaldehyde derivatives by the aid of Amberlyst 15 resin under certain conditions; secondly, preparing and detecting artificial antigens, to be more specific, respectively coupling the obtained haptens and bovine gamma globulin (BGG) by the aid of active ester and diazotization processes and preparing the aniline green artificial antigens which are corresponding complete antigens formed by aniline green-bovine gamma globulin. The Amberlyst 15 resin is used as a catalyst. The method has the advantages that animal immunization can be carried out on the aniline green artificial antigens to obtain corresponding aniline green antibodies, the method can be used for researching diversified aniline green immunoassay processes, and convenient, quick and accurate ways can be provided for aniline detection.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a preparation method of aniline green artificial antigen. Background technique [0002] Aniline green is also called malachite green in Chinese; China green; alkaline malachite green; For ethanol, methanol and amyl alcohol, the aqueous solution is blue-green, and the pH below 0.0 is yellow; the maximum absorption wavelength is 616.9nm. It is a toxic triphenylmethane chemical, which is not only a dye, but also a fungicide, and can cause cancer. Its structural formula is: [0003] [0004] The chemical functional group triphenylmethane in aniline green can cause cancer, and many countries have banned it, but fishermen still use it to prevent fungal infections in fish. It is also used by transporters as a disinfectant to prolong the survival time of fish in long-distance trafficking. [0005] At present, the detection of aniline green mainly relies on ...

Claims

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Application Information

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IPC IPC(8): C07K14/47C07C227/10C07C227/16C07C229/52
CPCC07K19/00C07C227/10C07C227/16C07K14/4717C07C229/52
Inventor 叶肖俊
Owner HANGZHOU LAIHE BIOTECH CO LTD
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