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Method for synthesizing rare ginsenoside CK (compound K) through biotransformation of natural saponins

A ginsenoside and biotransformation technology, applied in the fields of bioengineering and microbial fermentation, can solve the problems that product safety cannot be fully guaranteed, affect the competitiveness of product health functional components, and high cost of separation and purification. Promote and enhance the effect of health care functions

Active Publication Date: 2016-10-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the use of Penicillium and other inedible or certain harmful microorganisms to transform and produce ginsenoside CK results in that the safety of the product cannot be fully guaranteed, and the cost of separation and purification is high, which affects the product as a health-care functional ingredient in the market. Competitiveness

Method used

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  • Method for synthesizing rare ginsenoside CK (compound K) through biotransformation of natural saponins
  • Method for synthesizing rare ginsenoside CK (compound K) through biotransformation of natural saponins
  • Method for synthesizing rare ginsenoside CK (compound K) through biotransformation of natural saponins

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Experimental program
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Effect test

Embodiment 1

[0024] (1) Strain activation: The prepared activation medium was sterilized by high temperature and high pressure, cooled and poured on the plate, inoculated the cryopreserved Ganoderma sinensis CGMCC 5.69 strain on the sterilized activation medium plate, Incubate for 7 days in a 25°C incubator until the plate is covered with mycelium, and the plate is set aside.

[0025] Wherein, the composition of activation medium is: potato powder 10g / L, glucose 20g / L, agar 20g / L, water.

[0026] (2) Seed cultivation: take 500mL Erlenmeyer flasks, fill each bottle with 100mL seed medium, sterilize and cool under high temperature and high pressure, dig out 1.5cm×1.5cm activated bacteria blocks, and inoculate into 100mL seed medium In Erlenmeyer flasks, cultivated for 5 days at 25°C and with a rotary shaker at a rotational speed of 180rmp.

[0027] Among them, the seed medium is composed of the following components: glucose 20g / L, peptone 18g / L, potassium dihydrogen phosphate 3g / L, magnesiu...

Embodiment 2～8

[0036] (1) Strain activation: The prepared activation medium was sterilized and cooled by high temperature and high pressure, and the plate was poured, and the frozen preserved Ganoderma sinensis CGMCC 5.69 strain was inoculated on the sterilized activation medium plate, and the plate was incubated at 25 Cultivate in an incubator at ℃ for 7 days until the plate is covered with mycelium, and the plate is set aside.

[0037] Wherein, the composition of the activation medium is: potato powder 10g / L, glucose 20g / L, agar 20g / L.

[0038] (2) Fermentation culture: take a 250mL conical flask, put 50mL of fermentation medium into each bottle, sterilize and cool under high temperature and high pressure, dig out 1cm×1cm of activated bacteria block and inoculate it into a cone with 50mL of seed medium In the bottle, under the condition of 25°C and the rotation speed of the rotary shaker at 180rmp, cultivate for 4d, 5d, 6d, 7d, and then add natural saponin to 0.5mg / ml, 1.0mg / ml, 1.5mg / ml, ...

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Abstract

The invention discloses a method for synthesizing rare ginsenoside CK (compound K) through biotransformation of natural saponins. According to the method, Ganoderma sinensis is used as a fermentation bacterium for fermentation culture, the natural saponins are added to fermentation broth for continuous fermentation after fermentation is performed for at least three days, and the rare ginsenoside CK is obtained. The rare ginsenoside CK is obtained through fermentation of Ganoderma sinensis used as the fermentation strain and the natural saponins used as a substrate, the transformation rate is high, and the rare ginsenoside CK is safe and non-toxic; compared with other microorganisms, the medicinal and edible fungus is higher in safety and can produce more bioactive substances in the process of synthesis through biotransformation, and product popularization is easy.

Description

technical field [0001] The invention relates to the technical fields of bioengineering and microbial fermentation, in particular to a method for synthesizing rare ginsenoside CK through biotransformation of natural saponins. Background technique [0002] Food functional molecules produced by biosynthesis and transformation of medicinal and edible fungi not only have various functions of functional molecules synthesized by chemical synthesis or other microbial biosynthesis, but also have the following advantages: (1) Compared with chemical synthesis, the use of raw materials It is safe and non-toxic, and the by-products of the reaction process are safe and non-toxic, and the product safety is high; (2) It can synthesize a large amount of products that are difficult to synthesize by chemical methods; (3) The strains used are safe and usable, and the strains themselves have Certain health benefits; (4) raw materials widely exist in nature and are cheap; (5) fermentation product...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20
Inventor 陈启和吴嘉南牛永武胡竞进
Owner ZHEJIANG UNIV
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