Totally enclosed multi-target nucleic acid isothermal amplification and detection integrated device

Abstract

The invention discloses a totally enclosed multi-target nucleic acid isothermal amplification and detection integrated device, which comprises a reaction tube casing, wherein a main runner is formed in the middle of the reaction tube casing; six annular shunt runners, which communicate with the main runner, are uniformly distributed in the bottom of the reaction tube casing, and meanwhile, six detection tube walls are uniformly distributed around the reaction tube casing; the hollow bottoms of the detection tube walls communicate with the annular shunt runners; the tops of the detection tube walls are in the form of openings; the upper ends of the reaction tube casing and the detection tube walls are sealed by virtue of a tube casing cover; a reagent tube, along the main runner, is placed in the reaction tube casing; all reagents, required by a biological reaction, are encapsulated in the reagent tube; the top of the reagent tube is sealed by virtue of a reagent tube cover; and a piercing device, which is used for piercing the reagent tube so as to release the reagents after completing the biological reaction, is arranged on the side or at the bottom of the reaction tube casing. The device disclosed by the invention integrates amplification and detection; and the device has the advantages of being visible in detection result, good in sealing performance, low in false positive, rapid in reaction, capable of achieving multi-target detection and the like.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a fully enclosed multi-target nucleic acid isothermal amplification and detection integrated device. Background technique [0002] Loop-mediated isothermal amplification technology (LAMP) is a nucleic acid amplification technology that efficiently amplifies at a constant temperature, proposed by Japanese scholar Notomi et al. in 2000. This technique requires 4 different specific primers to specifically recognize 6 specific segments of the target nucleic acid under the action of Bst DNA polymerase with strand displacement activity under the condition of 60-65°C. Loop-mediated isothermal amplification technology has higher amplification efficiency than polymerase chain reaction (PCR) and other isothermal amplification technologies, and can amplify the existing nucleic acid concentration to 10% of the original nucleic acid concentration within 60 minutes. 9 ~10 10 tim...

AI Technical Summary

Problems solved by technology

[0003] As we all know, no matter for PCR technology or isothermal amplification technology, the current nucleic acid amplification technology needs to go through three steps: nucleic acid extraction, nucleic acid amplification and result detection, and each step requires the use of corresponding instruments, a series of operations The steps are relatively cumbersome and require the operation of professionals, and the detection time is long and inconvenient

Due to the special reagents and reaction primers of loop-mediated isothermal amplification, loop-mediated isothermal amplification has higher sensitivity than other amplification techniques, so it is also more susceptible to contamination

The cross-contamination between samples and the pollution caused by laboratory aerosol make the false positive of ring-mediated isothermal amplification extremely high, and the multi-step operation increases the possibility of experimental operation contamination

At the same time, most amplification reaction tests can only target a specific target nucleic acid, and cannot be applied to the detection of multiple target nucleic acids in complex samples

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