Reagent clamping box with magnetic bead transferring structures

Abstract

A reagent clamping box with magnetic bead transferring structures comprises at least one magnetic bead transferring component. Each magnetic bead transferring component comprises a first cavity, a second cavity and a spacer region, wherein the first cavity and the second cavity are in adjacent arrangement and partitioned by the spacer region, the spacer area is internally provided with a storage cavity, and the magnetic bead transferring structure used for transferring magnetic beads from the first cavity to the second cavity is arranged between the first cavity and the second cavity. Each magnetic bead transferring structure comprises a magnet, a first spacer and a second spacer, wherein the first spacer and the second spacer are matched with each other and embedded in the corresponding storage cavity and are opened singly and sequentially in magnetic bead transferring. According to the scheme, each magnetic bead transferring structure is arranged between the corresponding adjacent cavities to realize complete and efficient transferring of the magnetic beads and to-be-extracted materials adsorbed to the magnetic beads. In addition, the reagent clamping box is not only simple in structure but also convenient to operate and effective in reduction of complexity of equipment used with the reagent clamping box.

Description

technical field [0001] The invention relates to consumables used in the preparation and detection of nucleic acid or protein, in particular to a reagent cartridge provided with a magnetic bead transfer structure. Background technique [0002] With the transformation of the medical model and the continuous development of individualized medicine, the medical testing community urgently needs fast and accurate detection methods, and molecular detection plays a unique role. The basis of molecular detection is to analyze the genes and their expression products in the tissue cells, hair, anticoagulant blood or dried blood stains of the subject, as well as formaldehyde-fixed and paraffin-embedded tissues. ) detection can be accurately detected once the disease occurs or even before the symptoms, signs and biochemical changes appear. [0003] At present, molecular detection technologies mainly include nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip ...

AI Technical Summary

Problems solved by technology

At present, blood routine, cytology, pathology and immunology and other testing methods are all developing in the direction of automation, integration and standardization, but due to the technical complexity of molecular testing, the fully automated instrument platform "from sample to result" Few or many technical problems that are difficult to solve

For example, the spin column method or magnetic bead method used in the field of biotechnology for nucleic acid extraction generally requires four steps of lysis, binding, rinsing, and elution, plus subsequent nucleic acid molecule hybridization, polymerase chain reaction (PCR ) and biochips and other detection steps, it is very difficult to realize the fully automated instrument "from sample to result". It is obvious that the manual material transfer method used in the prior art is not only cumbersome to operate, but also time-consuming and labor-intensive.

What's more, the whole operation process is easy to cause pollution, which affects the purity of the extract, and it is difficult to transfer the material fully and efficiently, which affects the experimental results.

Therefore, in view of the technical complexity of molecular detection itself, there are few fully automated instrument platforms for "from sample to result" or there are many difficult technical problems. In order to avoid problems in the prior art such as high cost, poor sensitivity, complex structure, overly complicated operation and related detection equipment, etc.

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