Dendrobium huoshanense tetraploid cultivation method

A technology of Dendrobium huoshanensis and cultivation method, which is applied in the directions of horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of low productivity of Dendrobium huoshanense, the conventional growth cannot meet the market demand, etc., and achieves environmental friendliness and improves productivity. , the effect of broad application prospects

Inactive Publication Date: 2016-12-14
安徽斛生记生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In order to solve the technical problems in the prior art that the productivity of Dendrobium Huoshanense is low and conventional growth cannot meet the market demand, the present invention provides a method for cultivating tetraploids of Dendrobium Huoshanense. The tetraploid plants of Dendrobium Huoshanense grown by the method are due to the doubled, its medicinal ingredients increased significantly, and it has a broad application prospect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Soak the Dendrobium dendrobii capsules in detergent for 15 minutes, clean them, and sterilize them in 75% alcohol for 30 seconds, then sterilize them with 0.1% mercury chloride for 15 minutes, and finally wash them with sterile water 3 times.

[0025] Cut the cleaned capsules in a sterile culture dish, take out the seed embryos for aseptic culture, disperse the seed embryos in a small amount of sterile water, use a straw to absorb the sterile water containing the seed embryos, and inoculate them into B5+ NAA 0.2mg / L medium, cultured at 25°C for 15 days, then switched to light culture for 30 days, so that the seed embryos germinated into green seed embryo protocorms.

[0026] Then the protocorm was inoculated on the teething induction medium MS+6-BA 0.1mg / L+TDZ 0.3mg / L added with DMSO 3% and colchicine 0.03% for induction for 3 days, and then transferred to The polyploid plants of Dendrobium huoshanense were obtained by culturing on medium MS+6-BA 0.1 mg / L+TDZ 0.3 mg / L ...

Embodiment 2

[0029] Soak the Dendrobium dendrobii capsules in detergent for 15 minutes, clean them, and sterilize them in 75% alcohol for 30 seconds, then sterilize them with 0.1% mercury chloride for 15 minutes, and finally wash them with sterile water 3 times.

[0030] Cut the cleaned capsules in a sterile culture dish, take out the seed embryos for aseptic culture, disperse the seed embryos in a small amount of sterile water, use a straw to absorb the sterile water containing the seed embryos, and inoculate them into B5+ NAA 0.2mg / L medium, cultured at 25°C for 15 days, then switched to light culture for 30 days, so that the seed embryos germinated into green seed embryo protocorms.

[0031] Then the protocorm was inoculated on the teething induction medium MS+6-BA 0.3mg / L+TDZ 0.6mg / L added with DMSO 3% and colchicine 0.03% for induction for 3 days, and then transferred to The polyploid plants of Dendrobium huoshanense were obtained by culturing on medium MS+6-BA 0.3 mg / L+TDZ 0.6 mg / L ...

Embodiment 3

[0034] Soak the Dendrobium dendrobii capsules in detergent for 15 minutes, clean them, and sterilize them in 75% alcohol for 30 seconds, then sterilize them with 0.1% mercury chloride for 15 minutes, and finally wash them with sterile water 3 times.

[0035] Cut the cleaned capsules in a sterile culture dish, take out the seed embryos for aseptic culture, disperse the seed embryos in a small amount of sterile water, use a straw to absorb the sterile water containing the seed embryos, and inoculate them into B5+ On the medium of NAA0.2mg / L, culture at 25°C for 15 days, then switch to light culture for 30 days, so that the seed embryos germinate into green seed embryo protocorms.

[0036] Then the protocorm was inoculated on the tooth induction medium MS+6-BA 0.1mg / L+TDZ 0.3mg / L added with DMSO 5% and colchicine 0.06% for induction for 3 days, and then transferred to The polyploid plants of Dendrobium huoshanense were obtained by culturing on medium MS+6-BA 0.1 mg / L+TDZ 0.3 mg / ...

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Abstract

The invention provides a dendrobium huoshanense tetraploid cultivation method. Aiming at the special physiological feature of dendrobium huoshanense, a dendrobium huoshanense capsule is cleaned, sterilized, and then sheared open in a sterile culture medium, and an embryo is taken out for sterile cultivation and is germinated to a green embryo protocorm; then the protocorm is inoculated and induced in a germination induction culture medium added with dimethyl sulfoxide and colchicine, the protocorm is then transferred to a culture medium not containing the dimethyl sulfoxide and the colchicine to be cultured, and a dendrobium huoshanense tetraploid plant is obtained. The dendrobium huoshanense tetraploid plant cultured with the method is relatively strong in ecological adaptability and relatively good in tolerance of an adverse situation, the dendrobium huoshanense has a higher medicinal component due to the reduplication of chromosomes, and the method has a relatively wide application prospect.

Description

technical field [0001] The invention relates to a method for cultivating tetraploids of Dendrobium huoshanense. Background technique [0002] Dendrobium is a plant of the genus Dendrobium in the family Orchidaceae. It is a precious medicinal material in traditional Chinese medicine. Its active ingredients are mainly polysaccharides and alkaloids. Among them, the quality of Dendrobium depends largely on the content of polysaccharides in Dendrobium. Among the Dendrobium species, Dendrobium Huoshanense is the most famous. The pharmacological effects of polysaccharides in Dendrobium Huoshanense mainly include immunity, strength, anti-aging, and anti-tumor effects. [0003] Polyploid breeding is an important means of cultivating new plant varieties. Polyploid breeding originated in the early 20th century and has been widely used in the cultivation of new plant varieties. Due to the increase of genome ploidy, polyploid often shows the characteristics of huge size and enhanced res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/08
CPCA01H4/005A01H1/08
Inventor 韩冬
Owner 安徽斛生记生物科技有限公司
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