Dendrobium huoshanense tetraploid cultivation method
A technology of Dendrobium huoshanensis and cultivation method, which is applied in the directions of horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of low productivity of Dendrobium huoshanense, the conventional growth cannot meet the market demand, etc., and achieves environmental friendliness and improves productivity. , the effect of broad application prospects
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Embodiment 1
[0024] Soak the Dendrobium dendrobii capsules in detergent for 15 minutes, clean them, and sterilize them in 75% alcohol for 30 seconds, then sterilize them with 0.1% mercury chloride for 15 minutes, and finally wash them with sterile water 3 times.
[0025] Cut the cleaned capsules in a sterile culture dish, take out the seed embryos for aseptic culture, disperse the seed embryos in a small amount of sterile water, use a straw to absorb the sterile water containing the seed embryos, and inoculate them into B5+ NAA 0.2mg / L medium, cultured at 25°C for 15 days, then switched to light culture for 30 days, so that the seed embryos germinated into green seed embryo protocorms.
[0026] Then the protocorm was inoculated on the teething induction medium MS+6-BA 0.1mg / L+TDZ 0.3mg / L added with DMSO 3% and colchicine 0.03% for induction for 3 days, and then transferred to The polyploid plants of Dendrobium huoshanense were obtained by culturing on medium MS+6-BA 0.1 mg / L+TDZ 0.3 mg / L ...
Embodiment 2
[0029] Soak the Dendrobium dendrobii capsules in detergent for 15 minutes, clean them, and sterilize them in 75% alcohol for 30 seconds, then sterilize them with 0.1% mercury chloride for 15 minutes, and finally wash them with sterile water 3 times.
[0030] Cut the cleaned capsules in a sterile culture dish, take out the seed embryos for aseptic culture, disperse the seed embryos in a small amount of sterile water, use a straw to absorb the sterile water containing the seed embryos, and inoculate them into B5+ NAA 0.2mg / L medium, cultured at 25°C for 15 days, then switched to light culture for 30 days, so that the seed embryos germinated into green seed embryo protocorms.
[0031] Then the protocorm was inoculated on the teething induction medium MS+6-BA 0.3mg / L+TDZ 0.6mg / L added with DMSO 3% and colchicine 0.03% for induction for 3 days, and then transferred to The polyploid plants of Dendrobium huoshanense were obtained by culturing on medium MS+6-BA 0.3 mg / L+TDZ 0.6 mg / L ...
Embodiment 3
[0034] Soak the Dendrobium dendrobii capsules in detergent for 15 minutes, clean them, and sterilize them in 75% alcohol for 30 seconds, then sterilize them with 0.1% mercury chloride for 15 minutes, and finally wash them with sterile water 3 times.
[0035] Cut the cleaned capsules in a sterile culture dish, take out the seed embryos for aseptic culture, disperse the seed embryos in a small amount of sterile water, use a straw to absorb the sterile water containing the seed embryos, and inoculate them into B5+ On the medium of NAA0.2mg / L, culture at 25°C for 15 days, then switch to light culture for 30 days, so that the seed embryos germinate into green seed embryo protocorms.
[0036] Then the protocorm was inoculated on the tooth induction medium MS+6-BA 0.1mg / L+TDZ 0.3mg / L added with DMSO 5% and colchicine 0.06% for induction for 3 days, and then transferred to The polyploid plants of Dendrobium huoshanense were obtained by culturing on medium MS+6-BA 0.1 mg / L+TDZ 0.3 mg / ...
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