A kind of vegetative tissue culture rapid propagation method of excellent single plant of Paulownia

Abstract

The invention discloses an asexual tissue culture fast propagation method of a paulownia superior individual plant. The method comprises the steps of adopting a way of propagating buds by using buds, obtaining germ-free buds through sterilizing, and optimizing and adjusting components and proportion of a culture medium; screening to obtain a culture medium formula in a tissue culture propagation stage including initial bud induction, subculture multiplication, rooting culture and the like; accompanied by appropriate temperature and illumination, building a tissue culture fast propagation system of the paulownia superior individual plant. According to the method, a stem with axillary buds of the paulownia superior individual plant is used as an explant, so that the superior characteristics of the individual plant is saved at the greatest extent; a disinfectant is sprayed before collecting the explant, so that the contamination rate of explant sterilization is effectively reduced, is 2.3 percent minimally; a subculture multiplication culture medium has an obvious multiplication effect and achieves a multiplication coefficient being 13.2 to 15.8; the rooting culture period is short, a root system begins to germinate after rooting culture for 6 to 8 days, the rooting percentage achieves 98.5 to 100 percent, and after continuously culturing for 15 days, the rooting percentage can achieve 100 percent, so that the asexual tissue culture fast propagation method of the paulownia superior individual plant has better economic benefit, ecological benefit and social benefit.

Description

technical field [0001] The invention belongs to the technical field of asexual rapid propagation of plants, and relates to a method for asexual tissue culture rapid propagation of an excellent single plant of Paulownia. Background technique [0002] Paulownia (Latin name: Paulownia.), aliases: white flower paulownia, large fruit paulownia, empty paulownia, etc., is a deciduous tree with fast growth, strong resistance, wide application range, excellent material, straight wood texture, beautiful pattern, color and luster Pleasing to the eye, light and soft material, low density, stable size, no warping or cracking, excellent material characteristics for making plywood, jigsaw panels, and laminated materials; paulownia material has excellent thermal insulation performance, good resonance performance, high radiation damping, and internal friction Small, it is an excellent material for stringed instruments; paulownia wood has high wood fiber and is an excellent raw material for par...

AI Technical Summary

Problems solved by technology

[0003] At present, the cultivation of Paulownia is basically in a wild or semi-wild state, while conventional cutting propagation is difficult to take root; the germination rate of buried root propagation is low, and the collection of root segments is not easy; graft propagation leaves hidden dangers in terms of compatibility in the later stage and affects Determination of clones and good traits cannot be fully expressed in afforestation production