Detection method of gentamicin C1a
A technology for the detection of gentamicin and its detection method, which is applied in the field of detection of gentamicin C1a, can solve the problems of narrow linear range and limited application range, and achieve the effects of wide linear range, high accuracy and simple operation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 2
[0045] Reference example 2 (precision test)
[0046] Dilute the standard solution with a concentration of 18 mg / ml in Reference Example 1 into a standard solution with a concentration of 1.155 mg / ml with distilled water; then prepare the derivatization reagent according to the preparation method of the derivatization reagent in Reference Example 1, and follow the reference The derivatization reaction step and HPLC detection method in Example 1 were used for derivatization and detection. The injection was repeated 5 times, and the RSD was calculated. The results are shown in Table 1.
[0047] Table 1
[0048]
[0049] It can be seen from Table 1 that the derivatization and detection are carried out according to the preparation method of the derivatization reagent, the derivatization reaction steps, and the HPLC detection method in Reference Example 1, and the precision is relatively high.
[0050] Reference example 3 (standard product curve drawing)
[0051] Precisely dra...
Embodiment 1
[0055] Embodiment 1 (measure the concentration of gentamicin C1a after the sample to be tested is diluted)
[0056] Precisely draw the solution of the sample to be tested in Reference Example 1, and dilute it into solutions of different multiples, such as: 1, 1.2, 1.4, 1.6, 1.8, 2, 4, 6, 8, and 10 times. According to the preparation method of the derivatization reagent in Reference Example 1, the derivatization reaction steps, and the HPLC detection method, derivatization and detection are carried out, and the peak area obtained from the detection is found according to the standard curve in Reference Example 3. Corresponding concentration value, the The concentration of the sample to be tested can be obtained by multiplying the concentration value by the dilution factor corresponding to the sample to be tested.
[0057] Take the dilution factor as the X-axis, and the concentration of the sample to be tested as the Y-axis, and draw a curve. The results are shown in image 3 . ...
PUM
Property | Measurement | Unit |
---|---|---|
Molar concentration | aaaaa | aaaaa |
Wavelength | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com