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Method for inducing transdifferentiation of mesenchymal stem cells into skin stem cells

A technology of skin stem cells and stromal stem cells, which is applied in the field of inducing the transdifferentiation of mesenchymal stem cells into skin stem cells, can solve the problems of small number of skin stem cells, long time required, and low transformation rate, and achieves the goal of improving skin characteristics and delaying skin aging. Effect

Active Publication Date: 2017-01-11
黄兵 +1
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Problems solved by technology

[0006] In order to solve the above-mentioned technical problems, the object of the present invention is to provide a method for inducing human adipose-derived mesenchymal stem cells to transform into skin stem cells. Problems such as the small number and low purity of skin stem cells after growth and transformation

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  • Method for inducing transdifferentiation of mesenchymal stem cells into skin stem cells
  • Method for inducing transdifferentiation of mesenchymal stem cells into skin stem cells
  • Method for inducing transdifferentiation of mesenchymal stem cells into skin stem cells

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Embodiment Construction

[0038] The specific embodiment of the present invention is described in detail below in conjunction with accompanying drawing:

[0039] Such as figure 1 Shown, a kind of method of inducing mesenchymal stem cell transdifferentiation into skin stem cell, it comprises the steps:

[0040] S1, rapid isolation and purification of homogeneous adipose-derived mesenchymal stem cells;

[0041] S2, select a variety of small RNA molecules that can be used for epigenetic regulation of stem cells;

[0042] S3, assembling and transfecting nucleic acid polypeptide nanoparticles;

[0043] S4, preparing a medium for inducing transdifferentiation of mesenchymal stem cells into skin stem cells;

[0044] S5, activates a variety of related genes that guide the self-renewal and transdifferentiation of skin stem cells.

[0045] Such as Figure 2 to Figure 8 As shown, the specific steps and operations are as follows:

[0046] 1. Isolation, purification and culture of human AD-MSC:

[0047] Isol...

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Abstract

The invention discloses a method for inducing transdifferentiation of mesenchymal stem cells (AD-MSCs) into skin stem cells (AD-SSCs). The method specifically comprises: S1, rapidly separating and purifying homogeneous adipose mesenchymal stem cells; S2, selecting a variety of small RNA molecules stem capable of being used for stem cell epigenetic regulation; S3, assembling and transfecting nucleic acid polypeptide nanoparticles; S4, preparing a culture medium for inducing transdifferentiation of mesenchymal stem cells into skin stem cells; and S5, activating a variety of related genes for guiding the self-renewing and the transdifferentiation of the skin stem cells. According to the present invention, the human adipose mesenchymal stem cells with characteristics of easy obtaining, abundant source, no moral and ethical issue, safty and effectiveness are used as the induced object and are subjected to large-scale, rapid and high-purity transdifferentiation by transfecting the two small RNAs molecules under the effect of the special serum-free culture medium to obtain the AD-SSCs, wherein the induced skin stem cells are the same as the skin stem cells naturally produced in human body, and have the self-renewing ability and the potential for differentiation into the skin cells.

Description

technical field [0001] The invention relates to the technical field of stem cell culture transformation, in particular to a method for inducing the transdifferentiation of mesenchymal stem cells into skin stem cells. Background technique [0002] The main source of Adipose-derived stem cells (AD-MSCs) is white adipose tissue (WAT), which is widely distributed in the body and has abundant reserves. It is a kind of adult Mesenchymal stem cells (MSCs), These mesenchymal stem cells are widely distributed in different tissues, such as bone marrow, skeletal muscle, fat, heart muscle and liver. Studies have found that MSCs have the following characteristics: (1) The number of adipose tissue is huge, and 50ml of adipose tissue can produce about 5×10 5 Mesenchymal stem cells are about 500 times the number of stem cells obtained from bone marrow; (2) It is safe and easy to obtain materials, and a large number of cells can be obtained under local anesthesia, with strong in vitro expan...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/87C12N5/10
Inventor 黄兵殷勤伟
Owner 黄兵
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