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A kind of tissue culture rapid propagation method of Ficus chrysalis

A tissue culture and money technology, which is applied in the field of tissue culture and rapid propagation of Ficus chinensis, can solve the problems of not carrying out industrial production of Ficus chinensis, restricting tissue culture technology, and having no Ficus chinensis, so as to improve planting efficiency and solve the problem of The effect of severe shortage of supply and good economic benefits

Active Publication Date: 2019-02-22
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some ornamental aquatic plants such as water banyan, red pepper, and green pepper have successfully established a tissue culture technology system, there are no reports on the banyan tree, and no industrial production of the banyan tree
Ornamental aquatic plants grow in water all the year round, and the explants often carry a variety of bacteria, which makes sterile culture difficult and seriously restricts the application of tissue culture technology

Method used

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  • A kind of tissue culture rapid propagation method of Ficus chrysalis
  • A kind of tissue culture rapid propagation method of Ficus chrysalis
  • A kind of tissue culture rapid propagation method of Ficus chrysalis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A tissue culture rapid propagation method of Ficus chrysalis, comprising the following steps:

[0042] (1) Explant selection and disinfection:

[0043] ① Select the young and tender stems of Ficus chrysalis growing healthy and free from damage by diseases and insect pests as explants;

[0044] ②Remove excess leaves, wash with detergent to remove dirt on the surface of branches, and then dry them with paper towels;

[0045] ③ On the ultra-clean workbench, first soak it with 70% alcohol for 10-30s, and then rinse it once with sterile water for 3-5 minutes;

[0046] ④ Sterilize with 0.1-0.3% mercuric chloride solution for 10-15 minutes, and finally wash with sterile water for 3-5 times, each time for 3-5 minutes, to obtain sterilized explants;

[0047] (2) Preparation of improved MS medium:

[0048] The formulation of the improved MS medium is: in the improved MS medium FeSO 4 The concentration of the medium is 55.6mg / L, and the other components and concentrations are ...

Embodiment 2

[0065] The formulation of the improved MS medium is: in the improved MS medium FeSO 4 The concentration of the medium is 50mg / L, and the other components and concentrations are completely consistent with the original MS medium formula;

[0066] The concentration of 6-BA in the formula of the adventitious bud induction medium is 0.5mg / L, and the concentration of NAA is 0.05mg / L;

[0067] The concentration of 6-BA in the formula of the subculture medium is 1.5mg / L, and NAA is 0.05mg / L;

[0068] The concentration of NAA in the formula of described aseptic rooting medium is 0.5mg / L,

[0069] The rest of the steps are the same as those in Embodiment 1 and will not be repeated here.

Embodiment 3

[0071] The formulation of the improved MS medium is: in the improved MS medium FeSO 4 The concentration of the medium is 60mg / L, and the other components and concentrations are completely consistent with the original MS medium formula;

[0072] The concentration of 6-BA in the formula of the adventitious bud induction medium is 2mg / L, and the concentration of NAA is 0.5mg / L;

[0073] The concentration of 6-BA in the formula of the subculture medium is 2.5mg / L, and NAA is 0.5mg / L;

[0074] The concentration of NAA in the formula of described aseptic rooting medium is 3mg / L,

[0075] The rest of the steps are the same as those in Embodiment 1 and will not be repeated here.

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Abstract

The invention prepares a culture medium suitable for tissue culturing ficus deltoidea based on an MS culture medium according to the growth characteristics of the ficus deltoidea. The culture medium comprises a culture medium for adventitious bud induction, a culture medium for multiplication and a culture medium for rooting. By using the culture medium for adventitious bud induction, the culture medium for subculture multiplication, and the culture medium for sterile rooting provided by the invention, so that the propagation coefficient and the planting percent can be effectively improved; seedlings are robust, the time-to-market is shortened, and the planting benefit is improved; the problem of severe undersupply of the ficus deltoidea can be effectively solved, and favorable economic benefit is provided for a planter.

Description

technical field [0001] The invention relates to a method for rapid propagation of Ficus chrysalis through tissue culture, and belongs to the technical field of rapid propagation of plants. Background technique [0002] With the development of society and the improvement of living standards, aquariums are becoming more and more popular. Ornamental aquatic plants are an important component of the aquarium. Due to people's liking for aquatic plants, a large number of ornamental aquariums mainly based on aquatic plants are very popular. At present, there are more than 500 species of ornamental aquatic plants, most of which come from tropical regions such as America, Africa, and Southeast Asia. The little banyan, also known as the banyan, is a monocotyledonous plant belonging to the genus Ficus in the Araceae family. Compared with Ficus, the leaf shape is round. It is an emergent aquatic plant with slow growth and dark green leaves. It is one of the best choices for middle and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 王继华徐世强张木清
Owner GUANGXI UNIV