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A kind of rapid propagation method of tissue culture of fork stigma

A technology of tissue culture and fork stigma, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of no South American fork stylosa factory production, no South American fork stylus, etc., and solve the serious shortage of supply , improved planting efficiency and short production cycle

Inactive Publication Date: 2016-06-08
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although some ornamental aquatic plants such as water ficus, red pepper, green pepper, etc. have successfully established tissue culture technology systems, there are no reports related to South American fork stigma, nor is there any factory production of South American fork stigma

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A rapid propagation method for tissue culture of P. japonica, comprising the following steps:

[0021] (1) Explant selection and disinfection:

[0022] ① Select the young and tender branches of the vigorously growing Fork stigma as explants,

[0023] ② Wash the branches with a neutral detergent to remove the dirt on the surface of the branches, then remove the excess leaves, and dry the surface with a clean paper towel.

[0024] ③ On the ultra-clean workbench, first soak it with 70% alcohol for 10-30s, then rinse it with sterile water for 3 times, each time for 3-5min,

[0025] ④ Immediately transfer to 0.1-0.3% mercuric chloride solution for disinfection for 8-12 minutes, and finally wash with sterile water for 3-5 times, each time for 3-5 minutes, to obtain sterilized explants;

[0026] (2) Preparation of improved MS medium:

[0027] The formulation of the improved MS medium is: use Ca(NO 3 ) 2 4H 2 O instead of CaCl in the original MS medium 2 2H 2 O and addi...

Embodiment 2

[0037] A rapid propagation method for tissue culture of P. japonica, comprising the following steps:

[0038] (1) Explant selection and disinfection:

[0039] ① Select the young and tender branches of the vigorously growing Fork stigma as explants,

[0040] ② Wash the branches with a neutral detergent to remove the dirt on the surface of the branches, then remove the excess leaves, and dry the surface with a clean paper towel.

[0041] ③ On the ultra-clean workbench, first soak it with 65% alcohol for 10-30s, then rinse it with sterile water for 3-5 times, each time for 3-5min,

[0042] ④ Immediately transfer to 0.1% mercuric chloride solution for disinfection for 8-12min, and finally wash with sterile water for 3-5 times, each time for 3-5min, to obtain sterilized explants;

[0043] (2) Preparation of improved MS medium:

[0044] The formulation of the improved MS medium is: use Ca(NO 3 ) 2 4H 2 O instead of CaCl in the original MS medium 2 2H 2 O and adding ascorbic ...

Embodiment 3

[0054] A rapid propagation method for tissue culture of P. japonica, comprising the following steps:

[0055] (1) Explant selection and disinfection:

[0056] ① Select the young and tender branches of the vigorously growing Fork stigma as explants,

[0057] ② Wash the branches with a neutral detergent to remove the dirt on the surface of the branches, then remove the excess leaves, and dry the surface with a clean paper towel.

[0058] ③ On the ultra-clean workbench, first soak it with 70% alcohol for 10-30s, then rinse it with sterile water for 3-5 times, each time for 3-5min,

[0059] ④ Immediately transfer to 0.3% mercuric chloride solution for disinfection for 8-12min, and finally wash with sterile water for 3-5 times, each time for 3-5min, to obtain sterilized explants;

[0060] (2) Preparation of improved MS medium:

[0061] The formulation of the improved MS medium is: use Ca(NO 3 ) 2 4H 2 O instead of CaCl in the original MS medium 2 2H 2 O and adding ascorbic ...

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PUM

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Abstract

The invention discloses a quick propagation method for tissue culture of staurogyne sp. Accoridng to the method, a medium for adventitious shoot induction, a medium for subculture multiplication and a medium for sterile rooting, which are suitable for the tissue culture of the staurogyne sp, are prepared according to the growing characteristics of the staurogyne sp based on an MS medium. By using the medium for the adventitious shoot induction, the medium for the subculture multiplication and the medium for the sterile rooting, the propagation coefficient can be effectively increased, the production cost is lowered, the production cycle is short and the planting benefit is greatly improved. The method can be used for effectively solving the problem of severe undersupply of the staurogyne sp in summer, so that a good economic benefit provided for planters.

Description

technical field [0001] The invention relates to a method for rapid propagation of fork stigma by tissue culture, and belongs to the technical field of rapid propagation of plants. Background technique [0002] Ornamental aquatic plants play an important role in the aquarium. As people love aquatic plants, they have developed into ornamental aquariums dominated by aquatic plants. At present, there are more than 500 kinds of ornamental aquatic plants used in aquariums, most of which come from tropical regions such as America, Africa, and Southeast Asia. Stylophyllum is a dicotyledonous plant belonging to the genus Stylophyllia of the Acanthaceae, distributed in Brazil, Venezuela and other places. It is an emergent aquatic plant that grows slowly and can proliferate with stolons extending around. It can create a compact green foreground and is the best choice for foreground grass. Although it has just been introduced into our country in recent years, it has been recognized by...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王继华张木清徐世强
Owner GUANGXI UNIV