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A kind of tissue culture medium of asparagus leaves and rapid propagation method thereof

A culture medium, the technology of Longan leaves, applied in the tissue culture medium of Longi leaves and its rapid propagation field, can solve the problems of no Longi leaves, difficult to form scale, slow growth, etc., to solve the serious shortage of supply, good Economic benefits, the effect of increasing the reproduction coefficient

Active Publication Date: 2020-03-24
饶平源林药业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the slow growth of Longli Ye, it mainly relies on ramets for reproduction, which is inefficient and difficult to meet market demand
Large-scale and standardized planting is a development trend, but due to the seriousness of the number of seedlings, it is difficult to form a large scale at present, and a large number of seedlings need to be solved urgently in production
However, there is no report on the tissue culture of Asparagus leaves, and there is no industrialized production of Asparagus leaves.

Method used

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  • A kind of tissue culture medium of asparagus leaves and rapid propagation method thereof
  • A kind of tissue culture medium of asparagus leaves and rapid propagation method thereof
  • A kind of tissue culture medium of asparagus leaves and rapid propagation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] A tissue culture medium for leaves of Asparagus japonica, including adventitious bud induction medium, subculture propagation medium and aseptic rooting medium;

[0064] Adventitious bud induction medium: Add 6-BA, AD and IAA on the basis of the improved MS medium; among them, the concentration of 6-BA is 0.5mg / L, the concentration of AD is 0.2mg / L, and the concentration of IAA is 0.2 mg / L;

[0065] Secondary proliferation medium: Add 6-BA, AD and IAA on the basis of the improved MS medium, wherein the concentration of 6-BA is 0.5 mg / L, the concentration of AD is 0.2 mg / L, and the concentration of IAA is 0.2 mg / L mg / L;

[0066] Sterile rooting medium: Add IBA and IAA on the basis of the improved MS medium, wherein the concentration of IBA is 0.2mg / L, and the concentration of IAA is 0.5mg / L.

[0067] Modified MS medium: use Ca(NO 3 ) 2 4H 2 O instead of CaCl in MS medium 2 2H 2 O and NH 4 NO 3 , and then added riboflavin, the rest of the components had no change...

Embodiment 2

[0078] The characteristics of this embodiment are: in the adventitious bud induction medium, the concentration of 6-BA is 0.05mg / L, the concentration of AD is 0.1mg / L, and the concentration of IAA is 0.05mg / L;

[0079] Others are the same as in Example 1.

Embodiment 3

[0081] The characteristics of this embodiment are: in the adventitious bud induction medium, the concentration of 6-BA is 2mg / L, the concentration of AD is 0.5mg / L, and the concentration of IAA is 0.2mg / L;

[0082] Others are the same as in Example 1.

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Abstract

The invention discloses a culture medium for tissue culture of sauropus spatulifolius. The culture medium comprises an adventitious bud induction medium: 6-BA, AD and IAA are added on the basis of a modified MS medium; a subculture multiplication medium: 6-BA, AD and IAA are added on the basis of the modified MS medium; a sterile rooting medium: IBA and IAA are added on the basis of the modified MS medium; and the modified MS medium: replacing CaCl2.2H2O and NH4NO3 in the MS medium with Ca(NO3)2.4H2O, and adding riboflavin. The invention further discloses a rapid propagation method of tissue culture of sauropus spatulifolius. The culture medium can effectively improve the breeding coefficient and reduce the production cost; the production cycle is short; the planting benefit is greatly improved; the problem of serious supply shortage of the sauropus spatulifolius can be effectively solved, and growers are provided with good economic benefits.

Description

technical field [0001] The invention relates to a tissue culture medium of asparagus leaves and a rapid propagation method thereof, and belongs to the technical field of rapid propagation of plants. Background technique [0002] Asparagus leaves, also known as Longli leaves, cow ear leaves, dragon tongue leaves, and dragon flavor leaves, are mainly produced in Guangxi, Guangdong and other places, and belong to the Euphorbiaceae. Southern medicine and Zhuang medicine can clear lung and relieve cough, reduce phlegm and relieve asthma, treat lung-heat cough, bronchial asthma, acute bronchitis, upper respiratory tract inflammation, aphonia, tuberculosis, and sore throat. Small evergreen shrub with many branches, less twisted, alternate single leaves, slightly fleshy, oblanceolate, dark green or light blue-green, purple-red flowers, clustered or in cymes, and is also an excellent indoor foliage medicinal plant. [0003] Longli leaves are used as medicine, the quality is uneven, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 王继华蔡时可
Owner 饶平源林药业科技有限公司