97 results about "Riboflavinum" patented technology

The invention relates to the field of medical treatment, and discloses O2RF (at) MNs for treating keratectasia diseases and a preparation method of the O2RF (at) MNs. The O2RF (at) MNs comprises a patch base and a needle body array, the needle body array comprises a plurality of microneedles, and O2NE is loaded in the microneedles. The O2RF-coated MNs can form reversible micro holes in corneal epithelium, the blocking effect of hydrophobic corneal epithelium on hydrophilic RF is overcome, RF permeation is promoted, meanwhile, O2NE and PFD capable of releasing oxygen are loaded in the O2RF-coated MNs, due to the low polarity of fluorine, the oxygen can be combined and stored, after the microneedle is dissolved, the O2NE permeates into corneal stroma, oxygen is continuously released in the corneal stroma, and therefore the corneal epithelium can release oxygen continuously. In the crosslinking process, no extra device is needed to provide oxygen, so that the cornea crosslinking effect is enhanced. In addition, a tiny wound caused by the microneedle on the corneal epithelium can be completely healed within 12 hours, and corneal injury is small.

Systemic lupus erythematosus (SLE) is an autoimmune disease in which circulating immune complexes can cause different types of glomerulonephritis, according to immune deposits and to the type of glomerular cell injury. Proliferative lesions represent the most severe form of lupus nephritis (LN) and often lead to kidney failure and death. Mucosal-associated invariant T (MAIT) are a subset of innate-like T cells that recognize microbial-derived ligands from the riboflavin synthesis pathway. Now the inventors analyzed the phenotype and function of peripheral blood MAIT cells by flow cytometry in 26 patients with LN and in a control group of 16 healthy volunteers. The inventors found that MAIT cell frequencies are markedly reduced in blood of LN patients. More particularly, among LN patients, baseline MAIT cell frequency, Ki-67 expression evaluating proliferative activity and granzyme B production measuring cytotoxicity represent promising prognostic factors of renal response one year after induction therapy. In conclusion, the inventors showed that baseline frequency and cytotoxic profile of MAIT cells may represent a promising prognostic factor of renal remission one year after induction therapy.

An implantable collagen-based hydrogel material is produced by providing a solution having a collagen molecule concentration of 0.1 to 30 wt. % and a cellulose nanofiber concentration of 0 to 30 wt. %. To the solution is added a non-polymeric short-range carbodiimide crosslinking agent at a concentration of 1-30 wt. %, and riboflavin at a concentration of 0.1 to 10 wt. %. The solution is allowed to react, forming an intermediate hydrogel material, and thereafter the intermediate hydrogel material is exposed to ultraviolet A light, forming an implantable hydrogel material.

A method of controlling, limiting or preventing an infestation of a crop plant by a phytopathogenic fungi of the genus Fusarium by contacting plant propagation material, prior to or during sowing of the crop plant, with a composition comprising riboflavin.

A rinsing solution for photodynamic disinfection, in particular oral disinfection, including, at least one photosensitiser from the group comprising riboflavin 5′-phosphate sodium in a proportion of 0.2 to 0.6 wt. % and a photosensitiser other than riboflavin 5′-phosphate sodium in a proportion of 0.001 to 0.05 wt. %, at least one viscosity modulator from the group comprising glycerol in a proportion of 1.0 to 5.0 wt. % and hyaluronic acid sodium salt in a proportion of 0.01 to 0.3 wt. %, at least one biofilm inhibitor from the group comprising xylitol in a proportion of 7.0 to 25.0 wt. %, L-arginine in a proportion of 0.01 to 0.5 wt. % and urea in a proportion of 0.5 to 3.0 wt. %, with the remainder on a water base.

The application belongs to the technical field of bioengineering, and particularly relates to application of a riboflavin kinase / FMN adenosine transferase truncated protein and a mutant thereof in FMN synthesis. The application obtains a mutant with improved riboflavin kinase enzyme activity by truncating and mutating RibFM-1, and uses the mutant in FMN production. The FMN yield of the strain containing the truncated protein gene is increased by 49.14%, 33.97%, 43.58%, 22.42%, 76.31% and 113.70% respectively compared with the strain containing the non-truncated gene. It can be seen that the riboflavin kinase / FMN adenosine transferase can significantly improve the yield of FMN after being truncated or mutated, and has a wide application potential in FMN industrial production.

Provided herein are methods for inactivating a viral particle, the methods comprising contacting the viral particle with UV light in the presence of riboflavin. In some embodiments, the viral particle is a SARS-CoV-2 particle. Vaccine compositions comprising inactivated viral particles (e.g., inactivated SARS-CoV-2 particles) are also provided. In some embodiments, the vaccine compositions comprise an adjuvant capable of promoting a Th1-type immune response.

The invention provides a micro-current control method, a corneal iontophoresis system, electronic equipment and a medium, the micro-current control method is applied to a riboflavin iontophoresis device in corneal crosslinking, and the riboflavin iontophoresis device comprises a micro-current controller; the micro-current controller is electrically connected with the direct-current power supply and is used for converting constant current provided by the direct-current power supply into micro-current; the micro-current control method comprises the following steps: monitoring the current change of the output end of a micro-current controller in real time; monitoring the skin impedance change of the patient when the micro-current flows through the treatment area in real time; based on the current change and / or the skin impedance change, performing standard evaluation on the magnitude of the micro-current acting on the treatment area; and dynamically adjusting the micro-current which does not meet the standard so as to realize standard output of the micro-current. According to the application, by dynamically adjusting the magnitude of the micro-current, stable control of the current in the process of introducing riboflavin ions into the cornea is realized, and the safety and effectiveness of epithelial administration of riboflavin are improved.

A cultivation medium for Auricularia auricula that can increase riboflavin content, belonging to the technical field of breeding and cultivation of high-riboflavin Auricularia auricula strains, is composed of basswood sawdust, quicklime, gypsum powder, purified water and a fermented product. The fermented product, calculated by weight, is prepared by mixing 10 - 12 parts of Chrysanthemum morifolium Ramat., 5 - 8 parts of Ginkgo biloba leaves, 3 - 6 parts of Glycyrrhiza uralensis Fisch., 20 - 30 parts of pine cones and 15 - 18 parts of walnut shells into a mixed powder 1, adding chaff, corn flour and sucrose powder to form a mixed powder 2, and then fermenting with Hansenula anomala, Lactococcus lactis and Bacillus licheniformis after sterilization. The cultivation medium prepared by the present invention effectively increases the riboflavin content in Auricularia auricula, reaching more than 2 mg / 100 g of dry Auricularia auricula, shortens the cultivation cycle of Auricularia auricula, and at the same time, the dry Auricularia auricula yield reaches more than 120 g / bag. The above cultivation method can be used for the seedling cultivation of high-riboflavin Auricularia auricula strains, and high-quality strains of high-riboflavin Auricularia auricula can be obtained through the cultivation with this cultivation medium.

The present application belongs to the field of photochemical therapy, and particularly relates to a method for photochemical treatment of riboflavin based on multi-wavelength LED and application thereof in treatment of graft-versus-host disease. The present application selects different wavelength LED combinations as light sources to optimize the riboflavin photochemical method, controls the light intensity at 1000-25000 μW / cm², controls the irradiation time at 5-15 minutes, and controls the riboflavin concentration at 20-150 μM, thereby significantly improving the photochemical reaction effect. The method can efficiently induce PBMC apoptosis in a plasma environment, significantly reduces the levels of key pro-inflammatory cytokines such as TNF-α, IL-6 and IFN-γ, and has no significant influence on platelets, hemoglobin and blood clotting function, thereby providing a safe and controllable new cell preparation method for GVHD treatment.

The invention discloses application of riboflavin in preparation of medicines for treating colorectal cancer, belongs to the field of development of new application of medicines, and particularly relates to application of riboflavin to in-vitro stimulation of primary CD8 + T cells and improvement of the activity of the CD8 + T cells to improve the capacity of killing colorectal cancer cell lines. Meanwhile, the invention also proves that the activity of immune cells in the body of a colorectal cancer patient can be obviously activated through a conventional drinking water preparation and riboflavin in an animal model for the first time, particularly through the activity of CD8 + T cells, so that the capability of killing tumors is improved, and the volume and weight of the tumors are obviously reduced; the riboflavin can be used for preparing the medicine for treating the colorectal cancer.

Provided herein are compositions and methods for measuring the levels of flavins (e.g., in biological samples). In particular, provided herein are modified biotin-riboflavin conjugates and uses thereof (e.g., to measure flavin levels).

Methods of treating eyes are described. The method may include administering an agent capable of disrupting corneal cell junctures to a corneal surface of the eye, the corneal surface comprising an intact epithelium and stroma; and administering riboflavin to the corneal surface after or simultaneously with the agent. The riboflavin may diffuse through the eye to penetrate the stroma within 10 minutes of administration of the riboflavin.

An application of a riboflavin-loaded microneedle patch in the preparation of materials for treating keratoconus is disclosed. This microneedle patch, shaped like a contact lens, adheres comfortably to the ocular surface, with its base conforming to the conjunctiva and the microneedle array precisely oriented towards the cornea. Within a short period of application, the microneedles puncture the corneal epithelium and rapidly dissolve simultaneously, achieving efficient delivery of riboflavin to the stroma. Meanwhile, the dissolved base forms a protective film on the ocular surface. This microneedle patch enables safe and efficient delivery of riboflavin across the ocular barrier, effectively avoiding various risks associated with epithelial removal in traditional corneal cross-linking procedures, preventing large-area epithelial defects, preserving barrier integrity, and reducing the risk of postoperative infection. Furthermore, this microneedle patch achieves precise enrichment of riboflavin within the corneal stroma, overcoming the concentration uncertainty caused by passive diffusion after epithelial defects, which relies on traditional eye drops. This ensures that the riboflavin concentration in the stroma reaches the optimal photosensitization threshold, thereby maximizing cross-linking efficiency and demonstrating significant application potential in the clinical treatment of keratoconus.

The present disclosure relates to a plaque disclosing composition containing riboflavin 5-phosphate sodium, a method for disclosing a plaque, wherein the plaque is an acquired pellicle, and a method for collecting and analyzing an information about the amount and characteristics of dental plaque by analyzing the color and intensity of fluorescence emitted by riboflavin 5-phosphate sodium for effective treatment of dental plaque even at an early stage.

The invention discloses an S288C riboflavin kinase mutant and application thereof, and belongs to the technical field of riboflavin kinase. The amino acid sequence of the S288C riboflavin kinase mutant disclosed by the invention is as shown in SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5 or SEQ ID NO: 6. The original riboflavin kinase is subjected to site-directed mutagenesis and screening to obtain the S288C riboflavin kinase mutant which has high activity, high alkali resistance and high selectivity and is suitable for industrial application, and the S288C riboflavin kinase mutant has a wide application prospect when being used for preparing FMN.

The invention discloses Escherichia coli for fermentation production of riboflavin anti-bacteriophage, and belongs to the technical field of microorganisms. Receptor genes of bacteriophages are excavated through co-evolution of bacteriophages T1, T7, vBEcoMP251 and vBEcoMP255 and escherichia coli strains, the resistance of escherichia coli to the bacteriophages is enhanced by knocking out screened bacteriophage receptors on the genome level, and meanwhile, defense systems Gabija and KELM with the ability of resisting bacteriophages JNUWH1 and JNUWD and defense systems Shedu and Septu with the ability of resisting bacteriophages T4 are obtained through screening. Gene is integrated to a genome to strengthen the resistance of escherichia coli to bacteriophage, construction of the anti-bacteriophage chassis cell is completed, meanwhile, genes lacI, pfkA, edd and eda are knocked out, the anti-bacteriophage escherichia coli chassis cell for fermentation production of riboflavin is obtained, and a certain application prospect is achieved.

The invention discloses a riboflavin biosensor construction method based on an RNA element, and belongs to the field of synthetic biology. On the basis of an FMN riboswitch and a foothold switch, an expression platform of a wild type FMN riboswitch is artificially modified, a sensitive riboswitch biosensor is constructed, and efficient detection of the riboflavin concentration is achieved; the fluorescence value of a riboswitch of the detection strain constructed by the method under the riboflavin concentration of 1 mmol / L is 4.3 times of the fluorescence value under the riboflavin concentration of 0 mmol / L.

The application provides a resiquimod prodrug and a preparation method and application thereof. The resiquimot prodrug comprises a carrier and a riboflavin derivative loaded on the carrier. The carrier is obtained by bonding R848-TK and mPEG-PHEA. b The application uses R848 NPs as a carrier to physically load a liposoluble riboflavin derivative, and is used for constructing an ultrasound-activated resiquimot prodrug. The riboflavin derivative is a liposoluble substance, can be activated under ultrasound conditions, and then generates 1 O2, is used for cutting the TK bond, thereby releasing the active drug R848. The application can effectively enhance the tumor selectivity of the drug and realize a time-space controllable precise treatment by preparing a more specific ultrasound-activated resiquimot prodrug.

The invention provides a soluble microneedle patch for ultraviolet sclera crosslinking and a preparation method of the soluble microneedle patch, and relates to the field of biomedical materials. The soluble microneedle patch for ultraviolet sclera crosslinking comprises a microneedle body and a substrate layer, wherein the microneedle body and the substrate layer comprise polyvinyl alcohol and oxygen-carrying riboflavin lipidosome. After the soluble microneedle patch is applied, the bulbar conjunctiva does not need to be cut off in a large area, so that the operation time is shortened, the operation injury is reduced, the discomfort of a patient is relieved, and the operation safety is improved. Secondly, the height of the microneedle is individually designed according to different thicknesses of the sclera of the eyeball, so that the sclera is punctured in a safe and minimally invasive manner, and the operation safety is improved. And finally, after the soluble microneedle patch is applied, the automation of riboflavin perfusion is realized, the riboflavin solution does not need to be manually and frequently dropwise added, the surgical operation is simplified, and the surgical efficiency is improved.

The invention relates to an Alzheimer's disease biomarker based on intestinal flora metabolites and application of the Alzheimer's disease biomarker. The biomarker comprises any one or a combination of at least two of alpha-ketoglutaric acid, aminocaproic acid, arachidic acid, kynuraminic acid, riboflavin 5-monophosphate, ribonolactone, salicuric acid, serotonin, taurocholic acid-alpha-murine cholic acid, taurochenodeoxycholic acid, taurodeoxycholic acid, taurohyodeoxycholic acid and taurine-omega-murine cholic acid. According to the present invention, by detecting the specific metabolite level, the early diagnosis of Alzheimer's disease can be assisted, the non-invasive rapid detection is easily achieved, and the characteristics of timeliness, convenience, high specificity and high sensitivity are provided.

The application provides a Saccharomyces cerevisiae for fermentative production of riboflavin, a microbial inoculum, a fermentation method and application thereof, and relates to the field of microbial fermentation. The application provides a Saccharomyces cerevisiae XF-GT, with a preservation number of CCTCC NO: M20242498, which has been preserved in the China Center for Type Culture Collection on November 11, 2024. The application adopts the Saccharomyces cerevisiae XF-GT for fermentative production of riboflavin, optimizes carbon sources and nitrogen sources, and combines with medium feeding culture to make the riboflavin yield reach 45.97 g / L after 96 h of fermentation, and the conversion rate reach 12.47%, so that the fermentation production method has high yield, stable process and is beneficial to industrial production.

The riboflavin soaking device comprises a soaking ring, the soaking ring comprises a ring body, the surface of the bottom of the ring body is provided with a negative pressure groove which is concave inwards, the inner wall of one side of the negative pressure groove is provided with a communicating hole seat, one end of the communicating hole seat is communicated with a negative pressure adjusting handle, and the other end of the communicating hole seat is provided with a negative pressure adjusting rod. The negative pressure adjusting handle comprises a communicating pipe, one end of the communicating pipe is connected with the communicating hole seat in a communicating mode, a sealing piston is arranged in the communicating pipe and connected with the adjusting mechanism, a negative pressure groove is formed in the surface of the bottom of the ring body, and silica gel sealing rings are arranged on the edges of the two sides of the bottom end of the negative pressure groove to guarantee sealing performance. Meanwhile, the negative pressure groove is communicated and connected with the negative pressure adjusting handle, during use, the sealing piston can be driven by the adjusting mechanism to move in the communicating pipe, the negative pressure groove enters a negative pressure state, the whole ring body is adsorbed to the eyeball of a patient, and when the patient rotates the eyeball, the ring body can move along with the eyeball, so that medicine is prevented from flowing out and being wasted.

A fluorescent examination liquid containing water, riboflavin or rhodamine (B) as a fluorescent pigment dissolved in the water, and a deterioration preventive agent for preventing deterioration of the fluorescent pigment due to light, the deterioration preventive agent containing a p-hydroxybenzoic acid ester.

A preparation method of a bone nail based on photocuring silk fibroin comprises the following steps: firstly, uniformly mixing a post silk fibroin solution with a riboflavin aqueous solution to obtain a mixed solution, then adding a hydrogen peroxide solution into the mixed solution to obtain a precursor solution, enabling the concentration of the post silk fibroin solution to be 8-15wt%, enabling the concentration of the riboflavin aqueous solution to be 0.05-10mmol / L, and enabling the concentration of the precursor solution to be 0.5-10mmol / L; the volume ratio of the silk fibroin to the riboflavin aqueous solution is (5-20): 1, then pouring the precursor solution into a light-transmitting bone nail grinding tool, then placing the bone nail grinding tool under ultraviolet light for illumination so as to generate a photocuring reaction until the precursor solution is completely gelled, then placing the bone nail grinding tool in a freezing environment for demolding, and finally obtaining the bone nail grinding tool. And the bone nail blank taken out is placed in absolute ethyl alcohol to be dehydrated and then dried, and the bone nail is obtained. The design can simultaneously meet the advantages of mechanical strength, in-vivo degradability and harmlessness of degradation products to human bodies, and has a good market application prospect in the field of bone repair.

This invention belongs to the field of ophthalmic medication technology, specifically relating to a lyophilized formulation, dispersion method, and dispersion formulation of a metalloriboflavin composite nanomaterial. This invention discovers the -HPO4 group on riboflavin sodium phosphate. ‑ Metalloriboflavin composite nanomaterials obtained by coordinating metal ions on the group exhibit excellent transepithelial riboflavin delivery capabilities. Their transepithelial corneal crosslinking effect is comparable to that of classic corneal crosslinking schemes involving epithelial ablation. Further screening of the effects of preservation methods and dispersants on their transepithelial riboflavin delivery capabilities revealed that materials prepared by lyophilization and those dispersed in deionized water both retained the material's excellent transepithelial riboflavin delivery capabilities, comparable to the crosslinking effects of clinical epithelial corneal ablation. This has significant research implications for the long-term preservation, industrial production, and even future clinical applications of metalloriboflavin composite nanomaterials.

A sulfate reducing bacteria culture medium and a use thereof in culture and detection of sulfate reducing bacteria, and a method for detecting sulfate reducing bacteria. The culture medium comprises an electron mediator and L-cysteine; and on the basis of the total volume of the culture medium, in the culture medium, the content of the electron mediator is 10-100 mg / L, and the content of the L-cysteine is 1-20 mg / L, wherein the electron mediator is selected from riboflavin and / or flavin adenine dinucleotide. The culture medium provided by the present invention can significantly shorten the culture time required for strain purification and concentration measurement, and can significantly improve the detection efficiency.

The application discloses an EC riboflavin kinase mutant and application thereof, and belongs to the technical field of riboflavin kinase.The amino acid sequence of the EC riboflavin kinase mutant is shown in SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 or SEQ ID NO:6.The EC riboflavin kinase mutant with high activity, high alkali resistance, high selectivity and suitable for industrial application is obtained by site-directed mutagenesis and screening of the original riboflavin kinase, and the mutant can be used for preparing FMN and has wide application prospects.

The present invention pertains to a formulation designed for the treatment of acne, possessing potent antibacterial activity The invention additionally outlines a method for preparing this formulation tailored for acne treatment. The formulation, presented as a topical gel, incorporates a synergistic blend of doxycycline and riboflavin, along with key components such as carbopol, emollient, penetration enhancer, parabens, distilled water, and triethanolamine. Utilizing a 32 factorial design approach, the formulation is statistically optimized to achieve optimal characteristics. Notably, the formulation demonstrates a harmonious interaction between doxycycline and riboflavin, resulting in enhanced antibacterial efficacy. Furthermore, the formulation exhibits proven nonirritant properties and maintains stability during storage.

The application of an alkyl radical initiator in the preparation of a scleral collagen cross-linking drug and an oxygen-independent scleral collagen cross-linking method, which utilizes an alkyl radical initiator to cross-link type I collagen solution and scleral tissue, compared with the ultraviolet light / riboflavin cross-linking method, the reaction does not depend on the participation of oxygen, and may be more suitable for the sclera under the hypoxic state of myopia, and compared with the chemical cross-linking method, the cross-linking reaction is easier to control. The alkyl radical-induced collagen cross-linking has improved in terms of anti-enzyme degradation performance, thermal stability and biomechanical performance.