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Quick propagation method for tissue culture of hottonia inflata

A tissue culture, snowflakes technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of low reproduction coefficient and increase the cost of seedlings, etc., and achieves improved reproduction coefficient, short production cycle and good economic benefits. Effect

Inactive Publication Date: 2015-04-08
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reproduction of Snowflake Grass mainly depends on its tillers at present, and the reproduction coefficient is low, so a large number of seedlings are required in production, which increases the cost of seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A tissue culture rapid propagation method of Snowflake Grass, comprising the following steps:

[0022] (1) Explant selection and disinfection:

[0023] ① Select the young branches of Snowflake Grass as explants,

[0024] ②Wash the branches with a neutral detergent to remove the dirt on the surface of the branches, then remove the excess leaves, and dry the surface with a clean paper towel.

[0025] ③ On the ultra-clean workbench, first soak it with 70% alcohol for 10-30s, then rinse it with sterile water for 3-5 times, each time for 3-5min,

[0026] ④ Immediately transfer to 0.1-0.3% mercuric chloride solution for disinfection for 8-12 minutes, and finally wash with sterile water for 3-5 times, each time for 3-5 minutes, to obtain sterilized explants;

[0027] (2) Preparation of improved MS medium:

[0028] The formulation of the improved MS medium is: use Ca(NO 3 ) 2 4H 2 O instead of CaCl in the original MS medium 2 2H 2 O and adding ascorbic acid, the other c...

Embodiment 2

[0038] A tissue culture rapid propagation method of Snowflake Grass, comprising the following steps:

[0039] (1) Explant selection and disinfection:

[0040] ① Select the young branches of Snowflake Grass as explants,

[0041] ②Wash the branches with a neutral detergent to remove the dirt on the surface of the branches, then remove the excess leaves, and dry the surface with a clean paper towel.

[0042] ③ On the ultra-clean workbench, first soak it with 70% alcohol for 10-30s, then rinse it with sterile water for 3-5 times, each time for 3-5min,

[0043] ④ Immediately transfer to 0.1% mercuric chloride solution for disinfection for 8-12min, and finally wash with sterile water for 3-5 times, each time for 3-5min, to obtain sterilized explants;

[0044] (2) Preparation of improved MS medium:

[0045]The formulation of the improved MS medium is: use Ca(NO 3 ) 2 4H 2 O instead of CaCl in the original MS medium 2 2H 2 O and adding ascorbic acid, the other components were ...

Embodiment 3

[0055] A tissue culture rapid propagation method of Snowflake Grass, comprising the following steps:

[0056] (1) Explant selection and disinfection:

[0057] ① Select the young branches of Snowflake Grass as explants,

[0058] ②Wash the branches with a neutral detergent to remove the dirt on the surface of the branches, then remove the excess leaves, and dry the surface with a clean paper towel.

[0059] ③ On the ultra-clean workbench, first soak it with 70% alcohol for 10-30s, then rinse it with sterile water for 3-5 times, each time for 3-5min,

[0060] ④ Immediately transfer to 0.3% mercuric chloride solution for disinfection for 12 minutes, and finally wash with sterile water for 3-5 times, each time for 3-5 minutes, to obtain sterilized explants;

[0061] (2) Preparation of improved MS medium:

[0062] The formulation of the improved MS medium is: use Ca(NO 3 ) 2 4H 2 O instead of CaCl in the original MS medium 2 2H 2 O and adding ascorbic acid, the other compone...

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PUM

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Abstract

The invention discloses a quick propagation method for tissue culture of hottonia inflate, A medium for adventitious shoot induction, a medium for subculture multiplication and a medium for sterile rooting, which are suitable for the tissue culture of the hottonia inflata, are prepared according to the growing characteristics of the hottonia inflata based on an MS medium. By using the medium for the adventitious shoot induction, the medium for the subculture multiplication and the medium for the sterile rooting, the propagation coefficient can be effectively improved, the production cost is lowered, the production cycle is short and the planting benefit is greatly improved. The method can be used for effectively solving the problem of severe undersupply of the hottonia inflata in summer, so that a good economic benefit is provided for planters.

Description

technical field [0001] The invention relates to a tissue culture rapid propagation method of Snowflake Grass, which belongs to the technical field of plant rapid propagation. Background technique [0002] With the development of the national economy, aquariums are gradually becoming popular and more and more popular. Ornamental aquatic plants play an important role in the aquarium, and have developed to the ornamental stage of aquariums dominated by aquatic plants. At present, there are more than 500 kinds of ornamental aquatic plants used in aquariums, most of which come from America (crown), Africa (banyan), Southeast Asia (pepper grass) and other places. [0003] Snowflake grass (Hottonia inflat), often used as middle and foreground aquatic aquatic plants in aquarium scenery. Alternate leaves with 3-7 sub-pinnately parted, resembling crystallized patterns of snowflakes when viewed from above, and red-purple flowers on water. The water grass of snowflake grass is the sa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 徐世强王继华张木清
Owner GUANGXI UNIV