Treatment method for performing acute stress on megalobrama amblycephala

A technology of acute stress and treatment methods, applied in the field of fish farming, can solve the problems of fish death, low anti-stress ability, and large difference in stress response

Inactive Publication Date: 2017-02-01
TONGWEI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The bream has strong stress response and low anti-stress ability. During the process of pulling nets to sell fish and transportation, the fish body often has red hair and less or no mucus on the body surface due to stress, and sometimes even causes the fish body to die. , seriously affecting the economic benefits of farming
In previous studies on stress, measures such as fishing, hypoxia, and temperature control were often used to stimulate fish to produce stress, but the stress responses of different experimental batches were quite different, and it was not controllable

Method used

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  • Treatment method for performing acute stress on megalobrama amblycephala

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Selection and breeding of material fish: In June 2015, the group head bream was selected from the Nanquan Breeding Base of the Freshwater Fishery Research Center of the Chinese Academy of Fishery Sciences. The fish was healthy and domesticated in outdoor cages for 10 days, with a weight of (139±10) g. The body length is (22±2) cm, and the water temperature is about (25±2) ℃.

[0019] (2) Contrast injection: Divide 6 net cages into two groups, one group is the experimental group, the other is the injection group, the experimental group and the control group each have 75 fish, 25 fish in each box;

[0020] The experimental group was injected intraperitoneally with cortisol solution with a mass concentration of 5 mg / mL (solvent was ethanol), and the injection volume was 2 mL / kg body weight; the control group was injected with the same dose of ethanol.

[0021] The experimental group and the control group were sampled at 0, 2, 4, 8, 10, 12, 24 and 48 hours after injecti...

Embodiment 2

[0025] (1) Selection of material fish: In August 2015, the Nanquan Base of the Freshwater Fishery Research Center of the Chinese Academy of Fishery Sciences was selected to breed pomace. ±4) cm.

[0026] (2) Breeding of material fish: temporarily raise the material fish in a recirculation culture system for 2 weeks, control the water temperature at (25±1)°C, continue to oxygenate, the photoperiod is the same as natural light, and feed commercial pellet feed at 3% of body weight , 3 times a day.

[0027] (3) Cortisol injection: The experimental fish were intraperitoneally injected with a cortisol solution (2 mL / kg body weight, concentration 5 mg / mL, solvent is alcohol), and recorded as 0h after injection. Samples were taken at 0h, 2h, 4h, 8h, 12h, 24h, and 5d time points, and 5 fish were taken each time.

[0028] (4) Blood collection: Before sampling, the fish were anesthetized with 175 mg / L MS-222, and blood was collected from the tail vein. The same method as in Example 1 ...

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Abstract

The invention relates to a treatment method for performing acute stress on megalobrama amblycephala, and belongs to the technical field of fish aquaculture. The treatment method comprises the steps of selection of material fish, aquaculture of the material fish, cortisol injection and blood collection to obtain a sample. By limitation to the aquaculture water for experimental fish, the temperature and the density, stress caused by other factors can be avoided; a stress background value caused by fishing and injection is reduced by an anaesthesia method, and finally cortisol is injected quantitatively; compared with the conventional stress sampling method, the treatment method has the advantages of repetitiveness, liability for control and the like.

Description

technical field [0001] The invention relates to a treatment method for acute stress of bream, in particular to a method for injecting bream with exogenous stress hormones, so as to sample acute stress of bream, and the establishment has versatility and repeatability A sexual stress model belongs to the technical field of fish breeding. Background technique [0002] Under the high-density breeding mode, the stress caused by environmental or biological factors has a significant impact on the healthy growth and disease resistance of farmed animals, which has attracted widespread attention. Stress is the non-specific physiological response of the fish body to the stimulation of various adverse factors. The stress response of fish is similar to that of other vertebrates, and it is regulated by the hypothalamus-pituitary-kidney axis (HPI). Hormone (CRH) acts on the anterolateral pituitary gland where corticotropic cells are distributed, and adrenocorticotropic hormone is produce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10
CPCA01K61/00Y02A40/81
Inventor 习丙文陈凯高金伟薛春雨米海峰谢骏潘良坤
Owner TONGWEI
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