Tissue culture and rapid propagation of succulent plants

A technology for fast propagation of succulents and tissue culture, applied in the field of plant tissue culture, can solve the problems of large site requirements, low reproduction coefficient, long reproduction cycle, etc., and achieve the effects of short production cycle, high reproduction coefficient, and short culture time.

Inactive Publication Date: 2017-02-22
屏南县惠荣农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Small potted succulent plants are favored by consumers because they are easy to cultivate, have various overall shapes, colors and leaf shapes, and have high ornamental value. Some succule

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for tissue culture rapid propagation of succulents, comprising the following steps:

[0029] 1) Pre-processing

[0030] Select healthy young shoots or stems of 5cm-long succulents without pests, remove miscellaneous leaves and terminal buds, use physiological saline to clean the dust on the surface of the branches and cut them; then soak them in sodium hypochlorite aqueous solution for 8 minutes, use 1% chlorine Repeatedly rinse with mercury for 8 minutes, and place it on sterile filter paper to drain the water, and leave it for no more than 1 hour;

[0031] 2) Aseptic tissue establishment

[0032] On a sterile workbench, put the sprouts or stems collected in step 1) into a sterile glass petri dish, first add boric acid solution to cover the sprouts or stems, soak for 1min, then pour, then add phosphoric acid Repeated washing with salt buffer solution, and then use a hot air blower to dry the water on the surface of the young shoots or stems; then use a steri...

Embodiment 2

[0049] A method for tissue culture rapid propagation of succulents, comprising the following steps:

[0050] 1) Pre-processing

[0051]Select healthy young shoots or stems of 5cm-long succulents without pests, remove miscellaneous leaves and terminal buds, use physiological saline to clean the dust on the surface of the branches and cut them; then soak them in sodium hypochlorite aqueous solution for 8 minutes, use 1% chlorine Repeatedly rinse with mercury for 8 minutes, and place it on sterile filter paper to drain the water, and leave it for no more than 1 hour;

[0052] 2) Aseptic tissue establishment

[0053] On a sterile workbench, put the sprouts or stems collected in step 1) into a sterile glass petri dish, first add boric acid solution to cover the sprouts or stems, soak for 1min, then pour, then add phosphoric acid Repeated washing with salt buffer solution, and then use a hot air blower to dry the water on the surface of the young shoots or stems; then use a steril...

Embodiment 3

[0070] A method for tissue culture rapid propagation of succulents, comprising the following steps:

[0071] 1) Pre-processing

[0072] Select healthy young shoots or stems of 5cm-long succulents without pests, remove miscellaneous leaves and terminal buds, use physiological saline to clean the dust on the surface of the branches and cut them; then soak them in sodium hypochlorite aqueous solution for 8 minutes, use 1% chlorine Repeatedly rinse with mercury for 8 minutes, and place it on sterile filter paper to drain the water, and leave it for no more than 1 hour;

[0073] 2) Aseptic tissue establishment

[0074] On a sterile workbench, put the sprouts or stems collected in step 1) into a sterile glass petri dish, first add boric acid solution to cover the sprouts or stems, soak for 1min, then pour, then add phosphoric acid Repeated washing with salt buffer solution, and then use a hot air blower to dry the water on the surface of the young shoots or stems; then use a steri...

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PUM

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Abstract

The invention discloses a tissue culture and rapid propagation of succulent plants. The tissue culture and rapid propagation of succulent plants comprises the following steps: 1) pretreatment; 2) establishment of aseptic tissues; 3) differentiation culture and amplification culture of callus; 4) proliferation culture; 5) differentiation rooting; 6) acclimatization and transplantation; The tissue culture propagation technology provided by the invention has advantages of high propagation coefficient, short production cycle, simple program, easy operation and low cost, and is suitable for the rapid propagation of succulent plants.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of succulents. Background technique [0002] Succulentsrefers to a certain part of the plant's vegetative organs, such as stems or leaves or roots a few species have two parts, a type of plant that has developed parenchyma to store water, and appears plump and succulent in appearance. Most of them grow in arid areas or areas that are dry for a period of time in a year. For a long time every year, the roots cannot absorb water, and they only rely on the water stored in their bodies to maintain their lives. Small potted succulent plants are favored by consumers because they are easy to cultivate, have various overall shapes, colors and leaf shapes, and have high ornamental value. Some succulent plant manufacturers rely on leaf cuttings for propagation, which is not efficient, has low reproduction coefficient, long repr...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 张天进
Owner 屏南县惠荣农业科技有限公司
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