Method for counting algae cells and determining algae species on basis of line scanning Raman microscopy imaging

A microscopic imaging and Raman imaging technology, which is applied in Raman scattering, material excitation analysis, etc., can solve problems that cannot be directly counted or identified, and achieve the effect of rapid discriminant analysis

Active Publication Date: 2017-02-22
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

However, these methods have certain defects, such as the need for complex pretreatment (ultrasound, filtration, staining, etc.), which cannot be directly counted or identified. At the same time, the use of indirect methods requires the establishment of a standard curve in advance

Method used

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  • Method for counting algae cells and determining algae species on basis of line scanning Raman microscopy imaging
  • Method for counting algae cells and determining algae species on basis of line scanning Raman microscopy imaging
  • Method for counting algae cells and determining algae species on basis of line scanning Raman microscopy imaging

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Experimental program
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Embodiment 1

[0029] The first step is to prepare algae cell detection and counting plate by micro-processing method

[0030] On the silicon substrate, using photolithography method, according to figure 2 The counting plate structure processing algal cell detection chamber shown in the figure has a side length of 1mm*1mm, including 3*3 small detection chambers, and the depth of the detection chamber is 0.1mm. That is, when the algae liquid is full of the detection chamber, the volume of the algae liquid is 0.1mm 3 . Then cover the quartz cover glass on the detection chamber to prepare for the detection and counting of algae cells.

[0031] The second step, sample pretreatment and experimental preparation

[0032] Dilute the algae liquid sample to a certain number of times, shake well, use a pipette to take a certain amount of algae liquid, and drop it on the sample inlet 2 of the algae cell counting plate 1, and the algae liquid is dispersed to each detection chamber 4 under the action ...

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Abstract

The invention discloses a method for counting algae cells and determining algae species on basis of line scanning Raman microscopy imaging. The method comprises steps as follows: preparation of an algae cell detection counting board, pretreatment of algae cell samples, algae cell imaging and algae cell counting and algae specie determination based on algae cell Raman imaging. According to the method for counting the algae cells and determining the algae species on the basis of line scanning Raman microscopy imaging and multivariate data mining, algae cell counting and rapid determination and analysis of the algae species in an algae solution can be realized, and accurate counting of the algae cells and synchronous rapid recognition of the algae species are easy to realize.

Description

technical field [0001] The invention belongs to the technical field of environmental monitoring and protection, and in particular relates to a method for counting algae cells and identifying algae species based on line-scanning Raman microscopic imaging. Background technique [0002] With the rapid development of the economy, by the late 1990s, 88.6% of the investigated lakes in our country were in a state of eutrophication. Entering the 21st century, lake eutrophication presents a trend of rapid development. At present, the area of ​​eutrophic lakes in my country has reached 5000km 2 , the area of ​​lakes with conditions for eutrophication has reached 14,000km2. Because eutrophic water bodies are facing the problem of frequent outbreaks of freshwater algae blooms, using eutrophic water bodies as drinking water sources will pose a serious threat to the drinking water safety of Chinese residents. [0003] Among the water blooms caused by eutrophication in freshwater lakes,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 何石轩谢婉谊方绍熙王德强
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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