Method of using HLA-G positive mesenchymal stem cells to treat systemic lupus erythematosus

A kind of stem cell, lupus erythematosus technology, applied in the field of stem cell therapy, can solve the problems of difficulty in finding a transplant source, high mortality, and high cost

Active Publication Date: 2017-03-08
BOYALIFE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bone marrow-derived hematopoietic stem cell transplantation has been carried out at home and abroad to treat SLE, and has achieved certain curative effects, but there are problems such as difficulty in finding the source of transplantation and high cost, making it difficult to carry out clinically.
Moreover, autologous hematopoietic stem cell transplantation (HSCT, hematopoietic stem cell transplant) has a high recurrence rate, and allogeneic HSCT has a high mortality rate due to rejection and other reasons

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0132] Example 1: Preparation of human leukocyte antigen-G positive mesenchymal stem cells for the treatment of systemic lupus erythematosus (HLA-G+ mesenchymal stem cells or HLA-G+MSC)

[0133] Step (1), separating and culturing HLA-G+ umbilical cord mesenchymal stem cells

[0134] (11) After collecting the umbilical cord, put it into the transport solution (the composition of this transport solution is: 100ml low-sugar DMEM, 1ml double antibody, 1μg / ml amphotericin) and store it at low temperature, and bring it back to the laboratory for processing within 12 hours; The 5cm-long umbilical cord tissue in the base was placed on the ultra-clean workbench, and the D-HANKS solution repeatedly washed the lumen and blood vessels to wash away the residual blood until the washing solution was clear;

[0135] (12) Dissect the umbilical cord longitudinally, and bluntly tear off the blood vessels, urinary catheter, peritube and inner and outer membrane tissues;

[0136] (13) Use a mi...

Embodiment 2

[0167] Example 2: Treatment of Systemic Lupus Erythematosus Using Human Leukocyte Antigen-G Positive Mesenchymal Stem Cells

[0168] MRL / lpr mice are used as SLE animal models, and the test groups are as follows:

[0169] Treatment groups: HLA-G+MSC cell therapy group, portal vein injection group, tail vein injection group, and intratibial injection group;

[0170] Control group: MSC cell treatment group, portal vein injection group, tail vein injection group, and intratibial injection group; and

[0171] Blank control group: received normal saline in the same way.

[0172] Each group of animals n=30. Inject 2×10 5 corresponding cells.

[0173] (1) Investigate the living conditions of the mice:

[0174] Observing the survival rate of each group of mice, the results showed that: the MSC vein (portal vein and tail vein) treatment group could slightly prolong the survival period of lupus mice but was shorter than that of the intra-tibial injection group, and the 12-month s...

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PUM

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Abstract

The invention relates to a method of using HLA-G positive mesenchymal stem cells to treat systemic lupus erythematosus, in particular to, on one hand, a method of preparing human leukocyte antigen-G positive mesenchymal stem cells for treating systemic lupus erythematosus. The system includes following steps: (1), performing isolated culture on HLA-G+umbilical card mesenchymal stem cells; (2), cryopreserving the HLA-G positive mesenchymal stem cells; (3), resuscitating the HLA-G positive mesenchymal stem cells. Higher than 90% of the mesenchymal stem cells prepared by the method is positive to human leukocyte antigen-G. It has been already found that the HLA-G+umbilical card mesenchymal stem cells have excellent effect on treating systemic lupus erythematosus.

Description

technical field [0001] The invention belongs to the field of stem cell therapy, and relates to a method for treating systemic lupus erythematosus with mesenchymal stem cells, in particular to using human leukocyte antigen-G (usually abbreviated as HLA-G) positive mesenchymal stem cells (this HLA-G positive In the present invention, mesenchymal stem cells can be abbreviated as HLA-G+ mesenchymal stem cells) for treating systemic lupus erythematosus. Through the invention, a more effective treatment plan can be provided for systemic lupus erythematosus. Background technique [0002] Systemic lupus erythematosus (SLE for short) is a common immune disease. The etiology is still unclear, but it is characterized by dysfunction of the entire immune system, abnormal cell apoptosis, and abnormal lymphocyte function. Its serum contains a variety of autoantibodies represented by antinuclear antibodies, causing chronic damage to multiple system organs throughout the body. Traditional ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A01N1/02A61K35/28A61P37/02
Inventor 许晓椿肖海蓉刘冰
Owner BOYALIFE
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