Application of ilexgenin A in preparation of anti-atherosclerosis drugs
A technology of atherosclerosis and ilexine, applied in the field of medicine, can solve the problem of lack of IlexgeninA, and achieve the effect of reducing cardiovascular and cerebrovascular events, considerable social benefits, and improving atherosclerotic plaques
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Embodiment 1
[0021] Example 1: Human THP-1 mononuclear cell line was purchased from Shanghai Cell Bank, Chinese Academy of Sciences. THP-1 cells were cultured in 1640 medium (containing 10% FBS, 1% penicillin and streptomycin) at 37°C, 5% CO 2 cultured in an incubator. THP-1 mononuclear cells in the logarithmic growth phase were evenly spread into six-well plates (1×105 / ml) and cultured for 12 hours. Add 2ml of medium containing phorbol ester (100nM) to THP-1 mononuclear cells for induction for 48h; add 2ml of medium containing icolin A respectively, set up 4 concentration gradients (5, 10, 20, 40μM); 1h Afterwards, 2 ml of culture medium containing ox-LDL (20 μg / ml) was added and induced for 12 hours; RNA of THP-1 cells was isolated, and mRNA expression was measured by real-time quantitative PCR.
[0022] Conclusion: if figure 2 As shown, after incubation with Ox-LDL, the expressions of IL-1, TNF-α and IL-6 mRNA in THP-1 cells were significantly increased, and the levels of IL-1 and T...
Embodiment 2
[0023] Example 2: 20 ApoE knockout mice were randomly divided into two groups (n=10). After adapting to feeding for a week, feed high-fat feed. The mice in the test group were given 12 mg / kg / d of icolin A (Ilexin A dissolved in 0.5% CMC-Na aqueous solution) by intragastric administration; the mice in the model group were given an equal volume of 0.5% CMC-Na aqueous solution by intragastric administration for 16 weeks. In the blank group, 10 C57BL / 6J mice were fed with high-fat diet and fed with equal volume of 0.5% CMC-Na aqueous solution for 16 weeks. The mice were sacrificed and the aorta was taken to make slices, stained with H&E, and the area of aortic plaque was observed under a light microscope.
[0024] Conclusion: if image 3 As shown, compared with the model group, the aortic atherosclerotic lesion area of mice in the hollylin A intervention group was significantly reduced.
Embodiment 3
[0025] Example 3: Preparation of Capsules IlexgeninA 25g, starch 75g, thoroughly mix the dried starch and IlexgeninA, pass through a 120-mesh sieve, and mix thoroughly to prepare capsules, each containing 50mg of IlexgeninA.
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