Method for determining ancient silk fabrics by using peach gum gel slab electrophoresis

Abstract

The invention discloses a method for determining ancient silk fabrics by using peach gum gel slab electrophoresis. The ancient silk fabrics are degummed, dissolved, desalted, dialyzed and concentrated and then subjected to an electrophoresis experiment by using peach gum gel prepared in advance, wherein the peach gum gel has a space network structure, and the effect which is the same as that of sepharose gel after a series of treatment, and further has no pollution to the environment, thereby being higher in utilization values. Electrophoresis results of the ancient silk fabrics are subjected to enzymolysis by using a method of gel endoenzyme digestion and then mass spectrometry analysis by using HPLC-MS / MS, the results are compared with an amino acid sequence database, and the material types of the ancient silk fabrics are obtained according to amino acid sequences; according to the invention, the ancient silk fabrics are determined by using a proteomics method, so that on the one hand, the sensitivity is high and the operation is simple and convenient; on the other hand, the accuracy is high and the use amount of a sample is less.

Description

technical field [0001] The invention relates to a method for measuring ancient silk fabrics, in particular to a method for measuring ancient silk fabrics by electrophoresis on a peach gum gel plate. Background technique [0002] Silk is a symbol of ancient Chinese culture, and silk cultural relics are precious physical witnesses to the thousands of years of civilization and technological development of the Chinese nation. Solving the problem of the origin of silk in my country is not only the need to explore the origin of Chinese civilization, but also to "my country is the world's The statement that the world recognized the origin of silk” provides more convincing evidence. At present, commonly used methods for silk fabric detection, such as Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray diffraction, have low detection sensitivity and are greatly interfered by impurities. It is suitable for the analysis of well-preserved silk fabrics. Although it can ...

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Problems solved by technology

[0002] Silk is a symbol of ancient Chinese culture, and silk cultural relics are precious physical witnesses to the thousands of years of civilization and technological development of the Chinese nation. Solving the problem of the origin of silk in my country is not only the need to explore the origin of Chinese civilization, but also to "my country is the world's The statement that the world recognized the origin of silk” provides more convincing evidence. At present, commonly used methods for silk fabric detection, such as Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray diffraction, have low detection sensitivity and are greatly interfered by impurities. Suitable for analysis of well preserved silk fabrics

Although it can also provide some useful materials for the identification of silk fabric residues, it is difficult to make an accurate judgment on them. Proteomics is used to identify ancient silk fabrics. SDS-PAGE gel electrophoresis often shows texture and "ghost bands" , uneven bands and other phenomena affect the judgment of electrophoresis results