Method for determining ancient silk fabrics by using peach gum gel slab electrophoresis

A technology of peach gum gel plate and silk fabric is applied in the direction of measuring device, material analysis by electromagnetic means, instrument, etc., which can solve the problems of uneven strips, low detection sensitivity and large interference, and achieves high accuracy, The effect of high sensitivity and low sample usage

Inactive Publication Date: 2017-04-26
ZHEJIANG SCI-TECH UNIV
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Silk is a symbol of ancient Chinese culture, and silk cultural relics are precious physical witnesses to the thousands of years of civilization and technological development of the Chinese nation. Solving the problem of the origin of silk in my country is not only the need to explore the origin of Chinese civilization, but also to "my country is the world's The statement that the world recognized the origin of silk” provides more convincing evidence. At present, commonly used methods for silk fabric detection, such as Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray diffraction, have low detection sensitivity and are greatly interfered by impurities. Suitable for analysis of well preserved silk fabrics
Although it can also provide some useful materials for the identification of silk fabric residues, it is difficult to make an accurate judgment on them. Proteomics is used to identify ancient silk fabrics. SDS-PAGE gel electrophoresis often shows texture and "ghost bands" , uneven bands and other phenomena affect the judgment of electrophoresis results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: a kind of method that utilizes peach gum gel plate electrophoresis to finish ancient silk fabrics mensuration, this method adopts steps as follows:

[0037] a) Swell 20 g of dried peach gum solids picked in water, filter repeatedly until the liquid is clear, add hydrochloric acid to completely soak the peach gum, control the hydrolysis temperature at 80° C., and hydrolysis time for 0.5 h to obtain peach gum polysaccharide;

[0038] b) adding mass fraction to the peach gum solution is 20% H 2 o 2 200ml of the solution is used to decolorize the peach gum, and then use a dialysis bag with a molecular weight cut-off of 3500 for desalination treatment. After dialysis for 24 hours, use polyethylene glycol 6000 to concentrate, and put it into 100ml of SDS solution with a mass fraction of 0.5% for 6 hours;

[0039] c) Put a spacer between the two glue-making glass plates, put the glue-making frame on it, tighten the glue-making screws to clamp the glass plates, a...

Embodiment 2

[0046] Embodiment 2: a kind of method utilizing peach gum gel plate electrophoresis to complete ancient silk fabrics mensuration, the method adopts steps as follows:

[0047] a) Swell 35 g of dried peach gum solids picked in water, filter repeatedly until the liquid is clear, add hydrochloric acid to completely soak the peach gum, control the hydrolysis temperature to 90° C., and hydrolysis time to 0.75 h to obtain peach gum polysaccharide;

[0048] b) adding mass fraction to the peach gum solution is 25% H 2 o 2 300ml of the solution is used to decolorize the peach gum, and then use a dialysis bag with a molecular weight cut-off of 3750 for desalination treatment. After dialysis for 27 hours, use polyethylene glycol 6000 to concentrate, and put it into 200ml of SDS solution with a mass fraction of 1% for 7 hours;

[0049] c) Put a spacer between the two glue-making glass plates, put the glue-making frame on it, tighten the glue-making screws to clamp the glass plates, and pr...

Embodiment 3

[0056] Embodiment 3: a kind of method that utilizes peach gum gel plate electrophoresis to finish the ancient silk fabrics mensuration, the method adopts steps as follows:

[0057] a) Swell 50 g of dried peach gum solids picked in water, filter repeatedly until the liquid is clear, add hydrochloric acid to completely soak the peach gum, control the hydrolysis temperature at 100° C., and hydrolysis time for 1 hour to obtain peach gum polysaccharide;

[0058] b) adding mass fraction to the peach gum solution is 30% H 2 o 2 400ml of the solution is used to decolorize the peach gum, and then use a dialysis bag with a molecular weight cut-off of 4000 for desalination treatment. After dialysis for 30 hours, use polyethylene glycol 6000 to concentrate, and put it into 300ml of SDS solution with a mass fraction of 1.5% for 8 hours;

[0059] c) Put a spacer between the two glue-making glass plates, put the glue-making frame on it, tighten the glue-making screws to clamp the glass plat...

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Abstract

The invention discloses a method for determining ancient silk fabrics by using peach gum gel slab electrophoresis. The ancient silk fabrics are degummed, dissolved, desalted, dialyzed and concentrated and then subjected to an electrophoresis experiment by using peach gum gel prepared in advance, wherein the peach gum gel has a space network structure, and the effect which is the same as that of sepharose gel after a series of treatment, and further has no pollution to the environment, thereby being higher in utilization values. Electrophoresis results of the ancient silk fabrics are subjected to enzymolysis by using a method of gel endoenzyme digestion and then mass spectrometry analysis by using HPLC-MS / MS, the results are compared with an amino acid sequence database, and the material types of the ancient silk fabrics are obtained according to amino acid sequences; according to the invention, the ancient silk fabrics are determined by using a proteomics method, so that on the one hand, the sensitivity is high and the operation is simple and convenient; on the other hand, the accuracy is high and the use amount of a sample is less.

Description

technical field [0001] The invention relates to a method for measuring ancient silk fabrics, in particular to a method for measuring ancient silk fabrics by electrophoresis on a peach gum gel plate. Background technique [0002] Silk is a symbol of ancient Chinese culture, and silk cultural relics are precious physical witnesses to the thousands of years of civilization and technological development of the Chinese nation. Solving the problem of the origin of silk in my country is not only the need to explore the origin of Chinese civilization, but also to "my country is the world's The statement that the world recognized the origin of silk” provides more convincing evidence. At present, commonly used methods for silk fabric detection, such as Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray diffraction, have low detection sensitivity and are greatly interfered by impurities. It is suitable for the analysis of well-preserved silk fabrics. Although it can ...

Claims

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Application Information

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IPC IPC(8): G01N27/447
CPCG01N27/44795
Inventor 彭志勤古锦翠王秉万军民胡智文
Owner ZHEJIANG SCI-TECH UNIV
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