Method for cultivating virus-free seedlings of rhizoma atractylodis macrocephalae via two-step processes
A technology of virus-free seedlings and Atractylodes macrocephala, applied in horticultural methods, botany equipment and methods, gardening, etc., can solve the problems of difficulty in obtaining virus-free seedlings and low virus removal rate, achieve good virus-free effects and avoid damage Effect
Active Publication Date: 2017-05-17
GUIZHOU NORMAL UNIVERSITY
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Problems solved by technology
[0004] In production practice, sometimes such a problem is encountered: a plant with excellent growth performance is found in the field, and it is hoped to obtain the offspring of the plant through asexual reproduction for production, which requires the use of vegetative bodies for cultivation
It shows that the smaller shoot tips failed to cultivate successfully, although the larger shoot tips can induce budding, but the virus removal rate is low, and it is difficult to obtain virus-free seedlings
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[0042] Below in conjunction with preferred embodiments, the specific implementation, features and efficacy of the method for cultivating Atractylodes macrocephala virus-free seedlings according to the two-step method proposed by the present invention are described in detail as follows.
[0043] A two-step method for cultivating Atractylodes macrophage virus-free seedlings, comprising the following operating procedures:
[0044] 1. Utilize Atractylodes macrocephala terminal bud to establish aseptic culture line:
[0045] 1) Primary culture of Atractylodes macrocephala terminal buds: Select Atractylodes macrocephala plants with excellent growth performance in the field, cut off the about 2 cm long stem segment including terminal buds, remove the leaves, wash them with detergent, rinse them in running water for 30 minutes, and set aside. Bring the prepared materials into the ultra-clean workbench, cut off the terminal buds, soak in 75% alcohol for 30 seconds, rinse with sterile w...
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The invention discloses a method for cultivating virus-free seedlings by the aid of vegetative bodies of rhizoma atractylodis macrocephalae via two-step processes. The method includes firstly, establishing aseptic cultivation systems by the aid of terminal buds of the rhizoma atractylodis macrocephalae, to be more specific, disinfecting the terminal buds of the rhizoma atractylodis macrocephalae, then inoculating the terminal buds in primary cultivation media, and carrying out multiplication cultivation allowing continuously transferring the terminal buds so as to establish the aseptic cultivation systems with the terminal buds of the rhizoma atractylodis macrocephalae; secondly, carrying out virus-free cultivation on stem tips of aseptic seedlings, to be more specific, acquiring the aseptic seedlings from the established aseptic cultivation systems, stripping the stem tips with the sizes ranging from 0.3mm to 0.5 mm, inoculating the stem tips to induction cultivation media, detecting viruses by the aid of DAS-ELISA (double antibody sandwich-enzyme linked immuno sorbent assay) processes, discarding seedlings with the viruses, continuing to cultivate the seedlings (namely, the virus-free seedlings) without the viruses, transferring the virus-free seedlings into multiplication cultivation media, carrying out multiplication cultivation, transferring the virus-free seedlings to rooting cultivation media, carrying out rooting cultivation and exercising and transplanting rooted seedlings. The viruses are removed from a part of bud seedlings grown by the induction cultivation media. The method for cultivating the virus-free seedlings of the rhizoma atractylodis macrocephalae via the two-step processes has the advantages that damage to the stem tips due to disinfectants can be prevented, the small stem tips can be cultivated to develop into seedlings, and accordingly the virus-free seedlings can be obtained.
Description
technical field [0001] The invention belongs to the technical field of plant detoxification and tissue culture rapid propagation, and in particular relates to a method for cultivating detoxification seedlings by using Atractylodes macrocephala vegetative bodies through a two-step method. Background technique [0002] Atractylodes macrocephala Koidz (Atractylodes macrocephala Koidz) is a perennial plant of Compositae. It is widely used and used in large quantities. It is artificially cultivated in many places across the country. However, there are serious pests and diseases in the cultivation of Atractylodes macrocephala, and there is no specific agent for the prevention and treatment of viral diseases, and the method of detoxification is usually used for prevention and control. [0003] The laboratory has successfully obtained virus-free seedlings through embryogenic tissue culture and applied for an invention patent (patent number ZL201410041675.0). Embryogenic tissue in c...
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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 龚宁王红佳赵霰霖戚宇菡
Owner GUIZHOU NORMAL UNIVERSITY