Marine synbiotic additive, preparation method thereof, as well as application of marine synbiotic additive in breeding of stichopus japonicus
A technology of synbiotics and additives, applied in the fields of application, fish farming, climate change adaptation, etc., can solve problems such as restricting the healthy and sustainable development of the sea cucumber industry, destroying the balance of normal flora, and reducing production performance, so as to improve growth Performance and non-specific immune response, improving the intestinal micro-ecological environment of sea cucumber, and promoting the growth of animals
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[0044] 1. Preparation of marine synbiotic additives
[0045] Step 1: Ferment and cultivate Bacillus licheniformis WS-2 in the improved 2216E marine culture medium, the culture temperature is 28°C, pH 7.0, and the culture time is 48h to obtain the fermentation broth of Bacillus licheniformis WS-2, and adjust the Bacillus licheniformis WS -2 fermented liquid until the concentration of Bacillus licheniformis WS-2 is 1×10 10 CFU / mL, to obtain Bacillus licheniformis WS-2 bacterial liquid;
[0046] Peptone: fucoidan oligosaccharide: chitooligosaccharide: yeast extract: sand filtered sea water = 0.3:0.1:0.1:0.1:100(g:g:g:g:ml);
[0047] Step 2: Mix the above-mentioned Bacillus licheniformis WS-2 bacterial liquid with marine oligosaccharide prebiotics and skim milk powder, so that the mass concentration of marine oligosaccharide prebiotics is 5%, and the mass concentration of skim milk powder protective agent is 10%;
[0048] The above-mentioned marine oligosaccharide prebiotics inc...
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