Application of basil polysaccharide extract in preparation of medicine for treating pulmonary fibrosis

A technology of basil polysaccharide and pulmonary fibrosis, which is applied in the field of traditional Chinese medicine for the treatment of pulmonary fibrosis, can solve problems such as the fibrotic disease effect of basil polysaccharide extract, and achieve the effects of reducing morphological lesions, reducing collagen deposition and reducing content.

Active Publication Date: 2020-02-07
山东中医药大学资产经营有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report that basil polysaccharide extract has the effect of treating fibrosis diseases including IPF

Method used

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  • Application of basil polysaccharide extract in preparation of medicine for treating pulmonary fibrosis
  • Application of basil polysaccharide extract in preparation of medicine for treating pulmonary fibrosis
  • Application of basil polysaccharide extract in preparation of medicine for treating pulmonary fibrosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] In vivo pharmacodynamic study of basil polysaccharide extract in the treatment of pulmonary fibrosis

[0030] Bleomycin is the most commonly used inducer of pulmonary fibrosis model, which can cause pulmonary fibrosis in a small number of patients in clinical application, and has been used to prepare IPF models of various animals. The medicine of the present invention has therapeutic effect on the C57BL / 6 mouse IPF model animal induced by bleomycin.

[0031] 8-week-old male C57BL / 6 male mice were purchased from Jinan Pengyue Experimental Animal Breeding Co., Ltd. (certificate number: NO.37009200003495); the basil polysaccharide extract was extracted by the natural medicine laboratory of Shandong University of Traditional Chinese Medicine using the method of the present invention; Sodium pentobarbital: China National Pharmaceutical Group Shanghai Chemical Reagent Co., Ltd., prepare a 2% solution with normal saline and inject it intraperitoneally at 2ml / Kg body weight; bl...

Embodiment 2

[0039] In Vitro Pharmacodynamics of Basil Polysaccharide Extract in the Treatment of Pulmonary Fibrosis

[0040] The medicine of the present invention has therapeutic effect on common cell models of IPF.

[0041] The basil polysaccharide extract adopts the method of the present invention to extract by Shandong University of Traditional Chinese Medicine Natural Medicine Laboratory, and before use, it is prepared into a stock solution with a concentration of 10mg / mL with PBS damping fluid, subpackaged and frozen after sterilization; RPMI 1640 medium (batch number : 8115400) was produced by Gibco; newborn bovine serum was produced by Hangzhou Sijiqing Engineering Materials Co., Ltd.; recombinant human TGF-β (product number: 100-21, batch number: 0614209-1) was produced by PEPROTECH; RNA extraction reagent RNAiso Plus (Lot No.: AKA2603), Reverse Transcription Kit RT reagent Kit with gDNA Eraser (lot number: AK3001), qPCR kit, Premix Ex TaqTMⅡ (batch number: AK7001) was produce...

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Abstract

The invention discloses application of a Basil polysaccharide extract in preparation of a medicine for treating pulmonary fibrosis, and is new application of the Basil polysaccharide extract for treating the pulmonary fibrosis, and belongs to the technical field of traditional Chinese medicine for treating the pulmonary fibrosis. The Basil polysaccharide disclosed by the invention can lighten the pulmonary histomorphological lesion, lower the pulmonary tissue collagen deposition, reduce the pulmonary tissue hydroxyproline content, inhibit fibrotic morphological change of an A549 immortalized cell strain sourcing from a human-type II pulmonary epithelial cell, inhibit the col I gene expression of the A549 immortalized cell strain sourcing from the human-type II pulmonary epithelial cell, and lower the hydroxyproline content in the cell. The Basil polysaccharide extract has a certain effect of inhibiting fibrosis or collagen production from an IPF (idiopathic pulmonary fibrosis) model in vitro and vivo, and is a traditional Chinese medicine extract for treating the pulmonary fibrosis.

Description

technical field [0001] The invention specifically relates to the application of the basil polysaccharide extract in the preparation of pulmonary fibrosis medicine, is a new use of the basil polysaccharide extract for treating pulmonary fibrosis, and belongs to the technical field of traditional Chinese medicine for treating pulmonary fibrosis. Background technique [0002] The morbidity and mortality of fibrotic diseases continue to increase, and there are many people with fibrotic diseases. Although the understanding of the process of fibrosis has made significant progress in recent years, the pathogenic factors and pathological mechanisms of fibrotic diseases are still not fully understood, and there is currently no effective treatment to reverse or completely prevent the development of fibrotic diseases. Fibrotic diseases can involve multiple organs or systems, including lung, liver, kidney, cardiac fibrosis, and systemic sclerosis, multifocal fibrosis, scleroderma, etc.,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/53A61K31/715A61P11/00C08B37/00
CPCA61K31/715A61K36/53A61K2236/15A61K2236/331A61K2236/39A61K2236/51A61K2236/53C08B37/0003
Inventor 朱庆均张丹肖丽侯林张颖颖闫丹丹张成华李雅群
Owner 山东中医药大学资产经营有限公司
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