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Application of honeysuckle flower extract extracted with petroleum ether to preparation of pulmonary fibrosis treatment drug

A technology of pulmonary fibrosis and petroleum ether, applied in the field of traditional Chinese medicine for the treatment of pulmonary fibrosis

Active Publication Date: 2018-04-24
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report that the extract of honeysuckle petroleum ether has the effect of treating fibrotic diseases including IPF

Method used

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  • Application of honeysuckle flower extract extracted with petroleum ether to preparation of pulmonary fibrosis treatment drug
  • Application of honeysuckle flower extract extracted with petroleum ether to preparation of pulmonary fibrosis treatment drug
  • Application of honeysuckle flower extract extracted with petroleum ether to preparation of pulmonary fibrosis treatment drug

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Experimental program
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Effect test

Embodiment 1

[0028] In vivo pharmacodynamics study of honeysuckle petroleum ether extract in the treatment of pulmonary fibrosis

[0029] Bleomycin is the most commonly used inducer of pulmonary fibrosis model, which can cause pulmonary fibrosis in a small number of patients in clinical application, and has been used to prepare IPF models of various animals. The medicine of the present invention has therapeutic effect on the C57BL / 6 mouse IPF model animal induced by bleomycin.

[0030] 8-week-old male C57BL / 6 male mice were purchased from Jinan Pengyue Experimental Animal Breeding Co., Ltd. (certificate number: NO.37009200003495); honeysuckle petroleum ether part extract was adopted by the natural medicine laboratory of Shandong University of Traditional Chinese Medicine. Extraction; sodium pentobarbital: Shanghai Chemical Reagent Co., Ltd. of China Pharmaceutical Group, prepared a 2% solution with normal saline and injected intraperitoneally at 2ml / Kg body weight; bleomycin hydrochloride fo...

Embodiment 2

[0042] In Vitro Pharmacodynamics of Honeysuckle Petroleum Ether in the Treatment of Pulmonary Fibrosis

[0043] The medicine of the present invention has therapeutic effect on common cell models of IPF.

[0044] The honeysuckle petroleum ether extract is extracted by the method of the present invention in Shandong University of Traditional Chinese Medicine Natural Medicine Laboratory, and before use, it is prepared into a stock solution with a concentration of 10 mg / mL with no serum, subpackaged and frozen after sterilization; RPMI 1640 medium (batch number : 8115400) was produced by Gibco; newborn bovine serum was produced by Hangzhou Sijiqing Engineering Materials Co., Ltd.; recombinant human TGF-β (product number: 100-21, batch number: 0614209-1) was produced by PEPROTECH; RNA extraction reagent RNAiso Plus (Lot No.: AKA2603), Reverse Transcription Kit RT reagent Kit with gDNA Eraser (lot number: AK3001), qPCR kit, Premix Ex TaqTMⅡ (batch number: AK7001) was produced by...

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Abstract

The invention discloses an application of honeysuckle flower extract extracted with petroleum ether to preparation of a pulmonary fibrosis treatment drug, provides a novel application of the honeysuckle flower extract extracted with petroleum ether to pulmonary fibrosis treatment and belongs to the technical field of traditional Chinese medicine treatment of pulmonary fibrosis. The honeysuckle flower extracted with petroleum ether can relieve morphological lesions of lung tissue, reduce lung tissue collagen deposition, reduce content of hydroxyproline in the lung tissue, inhibit fibrosis morphological change of A549 immortalized cell lines sourced from human type-II pulmonary epithelial cells, inhibit type-I collagen gene expression of the A549 immortalized cell lines sourced from the human type-II pulmonary epithelial cells and reduce content of hydroxyproline in the cells. The honeysuckle flower extract extracted with petroleum ether has certain inhibition effect on fibrosis or collagen generation of in-vivo / in-vitro IPF models and is a potential Chinese herb extract for pulmonary fibrosis treatment.

Description

technical field [0001] The invention specifically relates to the application of honeysuckle petroleum ether part extract in the preparation of pulmonary fibrosis medicine, is a new use of honeysuckle extract for treating pulmonary fibrosis, and belongs to the technical field of traditional Chinese medicine for treating pulmonary fibrosis. Background technique [0002] The morbidity and mortality of fibrotic diseases continue to increase, and there are many people with fibrotic diseases. Although the understanding of the process of fibrosis has made significant progress in recent years, the pathogenic factors and pathological mechanisms of fibrotic diseases are still not fully understood. At the same time, most clinical trials have failed, and most fibrotic diseases currently lack effective treatments. . Fibrotic diseases include diseases involving multiple organs or multiple systems, such as lung, liver, kidney, and heart fibrosis, as well as systemic sclerosis, multifocal ...

Claims

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Application Information

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IPC IPC(8): A61K36/355A61P11/00
CPCA61K36/355A61K2236/333A61K2236/35A61K2236/39A61K2236/51
Inventor 容蓉朱庆均李雅群王旭张成华王萍
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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