Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for detecting multiple fat-soluble vitamins in feed

A technology for fat-soluble vitamins and vitamins, which is applied in the field of chromatographic detection and chromatographic detection and analysis of vitamins. It can solve the problems of low efficiency, lack, and long time-consuming detection and analysis of compound feed, etc., and achieve good separation effect, high detection and analysis accuracy, and analysis Results Precise and reliable results

Active Publication Date: 2019-10-18
NEW HOPE LIUHE +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The purpose of the present invention is to overcome the lack of simultaneous detection and analysis methods for multiple fat-soluble vitamins in compound feeds in complex situations in the prior art, so that the detection and analysis of compound feeds takes a long time and low efficiency, and provides a detection feed Multiple Fat Soluble Vitamins

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for detecting multiple fat-soluble vitamins in feed
  • A method for detecting multiple fat-soluble vitamins in feed
  • A method for detecting multiple fat-soluble vitamins in feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0044] The present invention will be further described in detail below in conjunction with test examples and specific embodiments. However, it should not be understood that the scope of the above subject matter of the present invention is limited to the following embodiments, and all technologies realized based on the content of the present invention belong to the scope of the present invention.

[0045] 1 Instruments and reagents

[0046] (1) Agilent 1260-6460 liquid chromatography-triple quadrupole tandem mass spectrometer with electrospray ion source ESI (Agilent Corporation, USA);

[0047] (2) AB-135 electronic analytical balance (Mettler Toledo Shanghai Co., Ltd.).

[0048] (3) LD5-2B centrifuge (Beijing Jingli Centrifuge Co., Ltd.).

[0049] (4) KQ-500DE CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0050] (5) Vortex mixer MS3 (IKA, Germany).

[0051] (6) Acetonitrile, methanol (chromatographically pure, Merck.

[0052] (7) Formic acid, ammoniu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
recovery rateaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for detecting multiple fat-soluble vitamins in a feed. The method comprises the steps of (1) preparing multi-vitamin standard solution which comprises at least two of vitamin A acetate, vitamin D3 and vitamin E acetate according to the detection requirements, and detecting the standard solution by adopting an HPLC-MS / MS to obtain a standard curve equation; (2) weighing a sample to a brown volumetric flask, adding an extract solution for ultrasonic extraction for 10-30 minutes, cooling to constant volume, centrifuging, filtering and processing by adopting a 0.22micron of organic phase filter membrane and carrying out analytical test on a chromatographic parameter and a mass spectrum detection parameter same as those in the step (1); and (3) calculating the content of multiple vitamins in the sample according to the detection results in the steps (1) and (2). According to the method for detecting multiple fat-soluble vitamins in the compound feed at the same time, detection and analysis of multiple different vitamins are completed in one detection and analysis process.

Description

technical field [0001] The invention relates to a chromatographic detection and analysis method, in particular to a chromatographic detection method for detecting vitamins, which has good detection and analysis efficiency for fat-soluble vitamins, and belongs to the technical field of chemical component analysis. Background technique [0002] Fat-soluble vitamins include VA, VD, VE, and VK. Because their chemical structures contain mostly hydrophobic hydrocarbon chains, they are insoluble in water and easily soluble in organic solvents. Due to the difference in solubility, its detection method is also different from that of water-soluble vitamins, especially the type of reagent used in sample pretreatment should be based on organic solvents. [0003] At present, the detection methods for water-soluble vitamins are relatively complete, such as titration, colorimetry, spectrophotometry, and the high-performance liquid chromatography and reversed-phase high-performance liquid c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 朱正鹏梁玉树何开蓉易锡斌张遨然汪春霞王虎周丽黄李蓉梁秋菊
Owner NEW HOPE LIUHE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com