Method for producing butanol

A technology for producing butanol and butanol, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of difficult to exceed the yield of butanol fermentation, high toxicity of production strains, poor chemical stability, etc., to achieve The effect of improving in-situ separation efficiency, reducing the steps of extracting butanol, and simplifying production operations

Inactive Publication Date: 2009-04-29
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some intractable problems in traditional ABE fermentation: high cost of growth medium, high toxicity of butanol at low concentration in the production process to the production strains, and the resulting high cost recovery of low product concentration
However, the problem of butanol's high toxicity inhibition to fermentation strains has not been solved, making it still difficult for butanol batch fermentation yield to exceed 12-14g / L
Permeation extraction and pervaporation are extraction and evaporation based on membrane separation technology respectively. By controlling the target products such as butanol to pass through the membrane preferentially, the goal of reducing product inhibition is achieved, which has the advantages of high efficiency and low energy consumption. However, the chemical stability of the membrane The characteristics of poor performance, weak mechanical strength and high price have become the bottleneck restricting its development.

Method used

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  • Method for producing butanol
  • Method for producing butanol
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, with CGM culture medium liquid-liquid extraction-continuous air stripping fermentation

[0039] Inoculate C.acetobutylicum SMB-1 CGMCC №.2287 into RCM medium, and culture it statically in a 37°C incubator until the logarithmic phase, as a fermentation seed solution.

[0040] The fermented seed liquid obtained above was inoculated into a 7L fermenter equipped with 3L of CGM medium according to the volume percentage of 5% for fermentation. Control the pH value in the fermenter to 5.0 (the pH is not controlled at the beginning of the fermentation, when the pH of the fermentation liquid drops to 5.0, the pH is automatically controlled at 5.0 by adding 4mol / L NaOH automatically), the fermentation temperature is 37°C, and the fermentation method is static fermentation. Counting from the beginning of fermentation, after 24 hours of fermentation, add 0.6 L of organic solvent (volume of organic solvent to fermentation medium) mixed with oleyl alcohol and decanol (...

Embodiment 2

[0046] Embodiment 2, with CGM culture medium liquid-liquid extraction-intermittent air stripping fermentation

[0047] The cultivation of the fermented seed liquid was the same as described in Example 1.

[0048] The fermented seed liquid obtained above was inoculated into a 7L fermenter equipped with 3L of CGM medium according to the volume percentage of 5% for fermentation. Control the pH value in the fermenter to 4.5 (the pH is not controlled at the beginning of the fermentation, when the pH of the fermentation broth drops to 4.5, the pH is automatically controlled at 4.5 by adding 4mol / L NaOH automatically), the fermentation temperature is 35°C, and the fermentation method is static fermentation. Counting from the beginning of fermentation, after 12 hours of fermentation, add 0.3 L of organic solvent (the amount of fermentation medium to organic solvent) mixed with oleyl alcohol and decanol (the volume ratio of oleyl alcohol and decanol is 1:1) to the reaction system. Th...

Embodiment 3

[0051] Embodiment 3, with cornstarch culture medium liquid-liquid extraction-intermittent air stripping fermentation

[0052] The cultivation of the fermented seed liquid was the same as described in Example 1.

[0053] The fermented seed liquid obtained above was inoculated into a 7L fermenter equipped with 3L cornstarch medium according to the volume percentage of 5% for fermentation. The fermentation temperature is 39°C, and the fermentation method is static fermentation. From the beginning of fermentation, after 30 hours of fermentation, 1.5 L of organic solvent (the volume ratio of oleyl alcohol and decanol is 10:1) mixed with oleyl alcohol and decanol (the volume ratio of oleyl alcohol and decanol) was added to the reaction system. The volume ratio is 2:1). At the same time, hydrogen is introduced through the ventilation pipe at the bottom of the fermenter. The ventilation time is 1h, and the hydrogen flow rate is 1.5L gas / min. The gas flow rate is the same as above; w...

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Abstract

The invention discloses a method for producing butyl alcohol. The method comprises the step of obtaining the butyl alcohol by utilizing butyl alcohol producing strain fermented on a fermentation medium, wherein in the method, a step of extracting the butyl alcohol from the fermentation product is also included. In the method, by liquid-liquid extraction and gas stripping, the butyl alcohol and the like is separated from the fermentation product in time, thereby lowering the inhibition action of the fermentation product on the fermentation reaction, on the basis of not adding equipment, the method improves in situ separation efficiency of the butyl alcohol, lowers the product inhibition of the butyl alcohol and improves the fermentation efficiency. Therefore, the method solves the problems of product inhibition, lower production strength and production efficiency of the butyl alcohol and higher recovery cost presently existing in the acetone butylalcohol fermentation, and is suitable for popularization and application.

Description

technical field [0001] The present invention relates to a method for producing butanol. Background technique [0002] Butanol is an important fine chemical raw material with a wide range of uses, widely used in the production of various plastics and rubber products and the manufacture of other organic compounds; bio-butanol is non-corrosive, low vapor pressure, and has a comparable calorific value to gasoline The advantages make it have the necessary conditions to replace motor gasoline. BP is cooperating with DuPont to develop a new generation of biofuels. The United States will also use biofuels such as biodiesel, ethanol and biobutanol to replace part of petroleum-based gasoline or Diesel is set as national policy. Therefore, the demand for butanol production in the market is getting higher and higher. [0003] The traditional method for producing butanol is ABE fermentation, which uses Clostridium acetobutylicum to produce acetone and butanol. In the 1920s, many count...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/16C12R1/145
Inventor 张延平王鑫昕李寅王少华
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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