Determinator for stomatal conductance of potatoes and determination method for stomatal conductance of potatoes

A technology of stomatal conductance and measurement method, which is applied to instruments, measuring devices, scientific instruments, etc., can solve the problems of expensive instruments, large experimental errors and high sensitivity, and achieve the effects of low production cost, simple mechanism and accurate results.

Active Publication Date: 2017-05-31
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first is to stick the surface of the blade with transparent adhesive tape, gently tear off the transparent adhesive tape and observe the marks left by the pores under the microscope; this method is simple to operate, but the clarity and accuracy are not high
The second method is to bring the leaves back to the laboratory, tear off the epidermis to make slices, and observe under a microscope; this method has high definition, but the guard cells are too long in vitro, and the stomata will still move, resulting in experimental errors Large, and it is difficult to completely tear off the epidermis
The third method is to use CO2 or O2 sensing technology to convert the gas concentration absorbed or released by the pores into an electrical signal for measurement; this technology has high sensitivity , but the instrument is expensive, maintenance and maintenance costs are high, and many laboratories cannot afford it

Method used

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  • Determinator for stomatal conductance of potatoes and determination method for stomatal conductance of potatoes
  • Determinator for stomatal conductance of potatoes and determination method for stomatal conductance of potatoes
  • Determinator for stomatal conductance of potatoes and determination method for stomatal conductance of potatoes

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Embodiment 1

[0031] The present embodiment provides a kind of potato stomatal conductance measuring instrument, such as figure 1 As shown, the analyzer includes: air flow system, blade fixing device and adjustment mechanism; The airflow system includes a needle 7, an airflow meter 9, an air pump 15, a voltage stabilizer 14, and an air pressure gauge 13; the blade fixing device includes an operating table 2, a locking knob 3, a sealing bowl 4, a locking rack 5, and a pressure plate 6; The adjustment mechanism includes a base 1 , a column 10 , a lifting platform 8 , a coarse accurate screw 11 and a fine accurate screw 12 . The needle head 1 is fixed on the lifting platform 8, and the lifting platform 8 is fixed on the column 10 and adjusted by the screw (coarse accurate screw 11 and fine accurate screw 12). The gas is driven by the air pump 15 into the regulator 14, the regulator 14 can adjust and control the air pressure, and can display the air pressure on the barometer 13; the gas with c...

Embodiment 2

[0040] The present embodiment provides a kind of potato stomatal conductance measuring method, such as figure 1 with 3 As shown, the assay method includes the following steps:

[0041] (1) Experiment preparation: Bring the above-mentioned potato stomatal conductance meter to the greenhouse or field, and place it on a portable folding stool. The height of the folding stool is equivalent to the height of the potato plant, and the leaves can be placed on the operating table without picking off the leaves. superior.

[0042] (2) Leaf pretreatment: Measure the leaf thickness with a vernier caliper, record the thickness, and avoid leaf veins when measuring. The upper surface of the blade is coated with petroleum jelly, and the edge of the sealing bowl 4 is also coated with petroleum jelly.

[0043] (3) Fix the blade: place the blade on the operating table (the blade is not separated from the plant), align the area to be tested with the sealing bowl, put down the pressure plate, a...

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Abstract

The invention provides a determinator for stomatal conductance of potatoes and a determination method for the stomatal conductance of the potatoes. The method comprises the steps of penetrating through epicuticle and palisade tissue of a leaf by needles, enabling the needles to reach an air chamber of spongy tissue, enabling air flow of a certain air pressure to enter the leaf from stomas and flow out of the needles, finally, determining the flow rate of gas flowing out of the needles, and converting the flow rate into air flow drag, i.e., the stomatal conductance. According to the determinator and the determination method, a mechanism is simple, the manufacturing cost is low, the operation is simple, and non-in-vitro measurement on the leaf can be achieved. The measurement process is almost free of injury to the leaf, and the measured leaf can also be used for carrying out measurement again, so that experiments can be repeated, and results are more accurate.

Description

technical field [0001] The invention relates to a plant physiology experiment device and method, in particular to a potato stomatal conductance measuring instrument and a method for measuring stomatal conductance using the measuring instrument. Background technique [0002] Stomata are small convex lens-shaped holes formed between plant guard cells. Stomata usually exist in the aerial parts of plants, especially on leaf epidermis, and also exist in small amounts on young stems and petals. The guard cells that form stomata differ from epidermal cells in that guard cells contain chloroplasts, are smaller in size and less in number, have poorly developed lamellar structures, and can perform photosynthesis to synthesize carbohydrates. Immediately below the stomata there are wide intercellular spaces (air cells) between the spongy tissues. Stomata serve as pathways for air and water vapor in plant gas metabolism such as carbon assimilation, respiration, and transpiration, and th...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): G01N33/00
CPCG01N33/0098
Inventor司怀军张宁唐勋周香艳文义凯杨江伟
OwnerGANSU AGRI UNIV