Kit for dry-type fluorescent quantum dot joint detection diagnosis of pancreatic cancer

Abstract

The invention discloses a kit for dry-type fluorescent quantum dot joint detection diagnosis of pancreatic cancer and a preparation method of the kit. The kit can be used for joint detection of three tumor markers Ca19-9, CEA, CA242 for clinical diagnosis of pancreatic cancer on the same detection strip, monoclonal antibodies of CA19-9, CEA and CA242 are fixed on a nitrocellulose film by means of an immunochromatography technique for preparation of a reaction film, the three monoclonal antibodies are marked with quantum dots emitting different light, the antibodies marked with the quantum dots are mixed and sprayed on a glass fiber film for preparation of a conjugate pad, and the conjugate pad, a sample pad and a water absorbing pad are combined to form a quantum dot immunostrip. The kit is prepared with a double-antibody sandwich method, three antigens in whole blood or serum and plasma are quickly detected, numerous defects of existing independent detection methods are avoided, the three tumor markers can be detected simultaneously with one drop of blood, the result is more direct, the sensitivity and the specificity are both remarkably improved, and accordingly, the kit has great potential in clinical application.

Description

technical field [0001] The invention belongs to the field of in vitro diagnostics, and relates to a tumor diagnosis kit, in particular to a pancreatic cancer dry-type fluorescence quantum dot joint detection diagnosis kit and a preparation method thereof. Background technique [0002] Pancreatic cancer refers to cancers that occur in the exocrine system of the pancreas. Most pancreatic cancers originate from the ductal epithelium and are ductal adenocarcinomas, and a few are cystadenocarcinomas and acinar cell carcinomas. later) tumors. Since there are no specific clinical symptoms in the early stage, the symptoms of pancreatic cancer depend on the location and size of the tumor. Clinically, more than 90% of the patients are diagnosed in the middle and advanced stages, and the best time for treatment has been lost, and the 5-year survival rate is only 5%. Among the common cancers in the world, pancreatic cancer ranks fourth in men and fifth in women, and its prognosis is p...

AI Technical Summary

Problems solved by technology

The detection of these three indicators in conventional laboratories or medical units is usually limited to a single kit, that is, one kit for the detection of one tumor marker. The disadvantages of this method are: 1. There are many detection steps, which are time-consuming and labor-intensive ; 2. At present, the three markers are detected by three single-index kits respectively, and then a comprehensive judgment is made. Due to differences in the kits of different manufacturers, and different kits from the same manufacturer may be different due to the different markers detected. There are differences in control conditions and parameters that lead to errors in the detection results. Such errors will have a greater impact on the specificity and sensitivity of the comprehensive analysis of the three markers, and may lead to a large difference in the judgment results.

[0004] The commercialized immunochromatographic test strips mostly use colloidal gold as a marker. Although the test results are visible to the naked eye, the colloidal gold cannot achieve accurate quantitative detection due to its large particle size, poor uniformity, and unstable immune markers. , low sensitivity and other defects

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