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Application of baicalein in preparation of drugs for treating pancreatic cancer

A cancer pancreatic cancer, baicalein technology, applied in the field of drug pharmacy, can solve the problem that the long-term tumor survival mechanism is not clear, etc.

Inactive Publication Date: 2017-07-07
FUDAN UNIV SHANGHAI CANCER CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There is research on the theory hypothesis of pancreatic cancer pathogenesis based on "damp-heat accumulation" as the core, and the method of "clearing heat and removing dampness" is established to treat pancreatic cancer with the traditional Chinese medicine compound Qingyi Huaji (QYHJ), and the results show that the clinical curative effect is obvious, about 8% Patients with advanced pancreatic cancer have achieved more than 5 years of tumor-bearing survival, but the long-term tumor-bearing survival mechanism has not yet been clarified

Method used

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  • Application of baicalein in preparation of drugs for treating pancreatic cancer
  • Application of baicalein in preparation of drugs for treating pancreatic cancer
  • Application of baicalein in preparation of drugs for treating pancreatic cancer

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Embodiment 1

[0020] 1. Experimental materials

[0021] 1.1 cells

[0022] Human pancreatic cancer PANC-1 cell line was purchased from ATCC (American Type Culture Collection).

[0023] 1.2 Animals

[0024] BALB / c nude mice, purchased from Shanghai Slack Experimental Animal Co., Ltd., license number: SCXK (Shanghai) 2007-0005, 4 weeks old, body weight: 18-22 g; female; kept in an SPF environment.

[0025] 1.2. Main reagents

[0026] 1.2.1 Monomer: Baicalein, a traditional Chinese medicine monomer, was purchased from Shanghai Traditional Chinese Medicine Standardization Research Center. 1.2.2 Reagents related to cell culture and in vitro detection: RPMI1640 and fetal bovine serum reagents were purchased from Gibco or Hyclon, glutamine was purchased from Hyclon, penicillin and streptomycin double antibodies, and sodium pyruvate were purchased from Sigma. bFGF, EGF, and B27 were purchased from Invitrogen.

[0027] 1.2.3 In Vitro Proliferation Detection Kit WST-8based Colorimetric Assay Cel...

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Abstract

The invention belongs to the field of pharmacy and relates to application of baicalein as an SHH (Sonic Hedgehog) signaling pathway inhibitor in the preparation of drugs for treating pancreatic cancer; results of animal experiments show that baicalein regulates Sonic Hedgehog signaling pathway to inhibit the proliferation of pancreatic cancer cells, induce apoptosis, and inhibit self-update of pancreatic cancer stem cells; the baicalein down-regulates IC50 of PANC-1 pancreatic cancer cells, weakening the in-vitro motor ability of the cells; the baicalein down-regulates the activity of CD44+ / CD24+PANC-1 pancreatic cancer cells and down-regulates surface marker CD24 and CD44 protein expression for pancreatic cancer cells, thereby reducing nuclear transcription factor OCT-4 and SOX-2 protein-level expression, inhibiting SHH, PTCH-1, SMO and GLI2, and reducing OCT-4 and SOX-2 protein-level expression. The baicalein may act as an SHH signaling pathway inhibitor to prepare drugs for treating pancreatic cancer.

Description

technical field [0001] The invention belongs to the field of drug pharmacy and relates to an inhibitor of human pancreatic cancer stem cell SHH signal pathway, in particular to the use of baicalein as an inhibitor of SHH signal pathway in the preparation of drugs for treating pancreatic cancer. Background technique [0002] The prognosis of pancreatic cancer is extremely poor, and it has become a consensus that it seriously endangers human health. Studies have shown that pancreatic cancer stem cells (CSCs) exist in pancreatic cancer tumors. Pancreatic cancer stem cells (CSCs) are a group of tumor cells that have functions similar to stem cells. The potential is reflected in unlimited self-renewal and differentiation into different types of tumor cells, which can lead to tumor recurrence, metastasis, and resistance to radiotherapy and chemotherapy, and ultimately affect the prognosis and survival of patients. Existing studies have shown that pancreatic cancer stem cells play ...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P35/00
CPCA61K31/352
Inventor 宋利斌刘鲁明
Owner FUDAN UNIV SHANGHAI CANCER CENT
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