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Applications of miR125b-5p in preparing drugs used for preventing endothelial cell damages

A technology of mir125b-5p and 1.mir125b-5p is applied in the application field of miR125b-5p in the preparation of anti-endothelial cell damage drugs, which can solve problems such as endothelial cell damage and achieve the effect of improving endothelial cells

Inactive Publication Date: 2017-07-14
XIAN MEDICAL UNIV
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Problems solved by technology

[0005] It has been reported in the literature that MicroRNA125b may promote macrophage activation and inhibit epithelial-mesenchymal transition, and it is proposed that MicroRNA125b-5p inhibits the proliferation of 3T3-L1 preadipocytes and promotes their adipogenic differentiation. Related reports on endothelial cell damage caused by oxidative stress

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  • Applications of miR125b-5p in preparing drugs used for preventing endothelial cell damages
  • Applications of miR125b-5p in preparing drugs used for preventing endothelial cell damages
  • Applications of miR125b-5p in preparing drugs used for preventing endothelial cell damages

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Embodiment Construction

[0027] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0028] In the present invention, a miR125b-5p consisting of 22 nucleotides is obtained by chemical synthesis, and its nucleotide sequence is shown in SEQ.ID.NO.1. Through further research, it was detected that miR125b-5p can significantly improve endothelial cell necrosis and apoptosis caused by oxidative stress, and further found that miR125b-5p can reduce endothelial cell necrosis and apoptosis by inhibiting Smad4.

[0029] The nucleotide sequence of miR125b-5p studied in the present invention is as follows:

[0030] UCC CUG AGA CCC UAA CUU GUG A (same as SEQ.ID.NO.1)

[0031] The specific experiments and results of miR125b-5p anti-endothelial cell necrosis and apoptosis and its inhibition of Smad4 expression provided by the present invention will be described in detail below.

[0032] 1. Detection of transfection efficiency of miRNA agom...

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Abstract

The invention discloses applications of miR125b-5p in preparing drugs used for preventing endothelial cell damages. The nucleotide sequence of miR125b-5p is represented by SEQ. ID. NO.1. It is found in experiments that miR125b-5p is capable of reducing endothelial cell necrosis and apoptosis caused by oxidative stress, and reducing expression of endothelial cell Smad4 expression, so that miR125b-5p can be used for preparing drugs used for preventing endothelial cell damages, and especially drugs used for preventing endothelial cell necrosis caused by oxidative stress.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of miR125b-5p in the preparation of anti-endothelial cell injury drugs. Background technique [0002] Endothelial cells provide oxygen and nutrients necessary for maintaining tissue homeostasis. Metabolic imbalance and environmental stimuli, such as cytokines or low shear stress, induce endothelial cell inflammation, increase endothelial cell permeability, promote cell proliferation, and eventually lead to cell death. These factors contribute to endothelial dysfunction, a key factor in the development of cardiovascular disease. In addition, superoxide anion hydroxyl radicals and hydrogen peroxide are detrimental to vascular endothelial cell function. It is widely believed that elevated levels of intracellular ROS damage various cellular components and block their physiological functions, leading to various oxidative stress-related diseases, such a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7088A61K48/00C12N15/113A61P9/00A61P39/06
CPCA61K31/7088C12N15/113C12N2310/141
Inventor 魏明甘露苏兴利陈玉龙梁飞孔令恒常晋瑞侯进胥晓丽刘彦彤
Owner XIAN MEDICAL UNIV
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