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Application technology for promoting tea selenium enrichment with endogenous spirillum in tea tree

An application technology, the technology of spirulina, is applied in the fields of application, cultivation, agriculture, etc. It can solve the problems not involved in the application of endophytic spirulina in tea trees, and achieve the effects of easy scale-up of production, strong reduction ability, and labor saving

Inactive Publication Date: 2017-07-21
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The literature "Microbiological characteristics of two strains of endophytic spirulina in tea trees" (Wang Ting et al., Journal of Microbiology 2014 No. 4, 2014.04.04, P424-432) described in detail the microbiological characteristics of endophytic spirulina in tea trees, but Not involving the application of endophytic spirulina in tea trees

Method used

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  • Application technology for promoting tea selenium enrichment with endogenous spirillum in tea tree
  • Application technology for promoting tea selenium enrichment with endogenous spirillum in tea tree
  • Application technology for promoting tea selenium enrichment with endogenous spirillum in tea tree

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take 5 kg of Enshi selenium-containing soil, the selenium content is 0.622 mg / kg, put it into flowerpots (pot mouth diameter 28cm, pot bottom diameter 25cm, height 27cm) and plant two-year-old Castanopsis chinensis tea seedlings, 5 plants per pot, put Grow under natural environmental conditions. After surviving, grow 3 new leaves, inject and inoculate the cultured tea tree endophytic Helix spirulina at the middle and lower end of the stem (3-5cm away from the soil surface), the inoculum amount is 200μl, and the bacterial content is 1.02×10 8 cfu / mL. The control group was injected with an equal volume of PBS (phosphate buffer pH 7.2). After the injection, the wound was sealed with sterile cotton balls. There were 20 pots in each of the experimental group and the control group, with a total of 100 plants in each group. Allow it to continue growing after inoculation. Samples were taken at 30, 60, 90 and 150 days respectively, and 25 plants were randomly taken out in ea...

Embodiment 2

[0028] Add 4.39 mg selenate to 5 kg Enshi selenium-containing soil (0.622 mg / kg selenium) to make the selenium content of the soil 1.5 mg / kg. After potting, plant the two-year-old Castanopsis chinensis tea seedlings, 5 plants per pot, and place them to grow under natural environmental conditions. After surviving, grow 3 new leaves, inject and inoculate the cultured tea tree endophytic Helix spirulina at the middle and lower end of the stem (3-5cm away from the soil surface), the inoculum amount is 200μl, and the bacterial content is 1.02×10 8 cfu / mL. The control group was injected with an equal volume of PBS (phosphate buffer pH 7.2). After the injection, the wound was sealed with sterile cotton balls. There were 20 pots in each of the experimental group and the control group, with a total of 100 plants in each group. Allow it to continue growing after inoculation. Samples were taken at 30, 60, 90 and 150 days respectively, and 25 plants were randomly taken out in each gro...

Embodiment 3

[0031] Add 14.39 mg of selenate to 5 kg of Enshi selenium-containing soil (0.622 mg / kg selenium) to make the selenium content of the soil 3.5 mg / kg. After potting, plant the two-year-old Castanopsis chinensis tea seedlings, 5 plants per pot, and place them to grow under natural environmental conditions. After surviving, grow 3 new leaves, inject and inoculate the cultured tea tree endophytic Helix spirulina at the middle and lower end of the stem (3-5cm away from the soil surface), the inoculum amount is 200μl, and the bacterial content is 1.02×10 8 cfu / mL. The control group was injected with an equal volume of PBS (phosphate buffer pH 7.2). After the injection, the wound was sealed with sterile cotton balls. There were 20 pots in each of the experimental group and the control group, with a total of 100 plants in each group. Allow it to continue growing after inoculation. Samples were taken at 30, 60, 90 and 150 days respectively, and 25 plants were randomly taken out in e...

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Abstract

The invention discloses a strategy and an application technology, wherein endogenous spirillum in a tea tree is used to promote selenium conversion and enrichment of the tea tree, so the content of organic selenium in tea leaves can be increased. Biological characteristics that the endogenous spirillum in the tea tree has reciprocal symbiosis with the tea tree and the selenium reducing capacity is high are used, and the endogenous spirillum in the tea tree is led into tea seedlings for fixed plantation, so that physiological poison effects of selenate absorbed by roots on the tea tree can be reduced; and selenium metabolism products are provided to the tea tree for direct use, so that enrichment of the organic selenium in the tea leaves can be promoted. By the technology, the selenium content in the tea leaves can be increase by two to three times in the tea tree which is planted in low-selenium soil or high-selenium soil or a tea garden with application of selenium fertilizer. According to the invention, technical processes designed according to field land tea tree cultivation can be operated easily; repeated inoculation and special management are not needed; and selenium enrichment effects are significant in the tea leaves.

Description

technical field [0001] The present invention relates to the technology of promoting the enrichment of organic selenium in tea by using endospira spirulina of tea tree, especially a method of transforming toxic selenate / selenite absorbed by plants from soil by endogenous spirulina of tea tree and promoting Method for the enrichment of organic selenium in tea leaves. Background technique [0002] Selenium is one of the essential trace elements for humans and animals, and has a very close relationship with the body's antioxidant capacity, immune function, anti-virus, and anti-cancer effects. Selenium in soil is mainly in the form of SeO 4 2- and SeO 3 2- It exists in the form of selenium and is soluble in water, and can be directly absorbed by the roots into the plant body and converted into organic selenium. Organic selenium can be effectively utilized by humans or animals. However, high concentrations of selenate have obvious toxicity to animals and plants. In view of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01G9/10
CPCA01G17/005
Inventor 王行国徐萧刘伟林程伟尤恒陈昌梅李亚东
Owner HUBEI UNIV
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