Molecular identification method and system for eucaryon source components

A molecular identification, eukaryotic technology, applied in the field of molecular identification methods and systems for eukaryotic-derived components, can solve the problems of high false negative rate, low throughput, poor versatility, etc., and achieves strong versatility, wide range, Variety of effects

Inactive Publication Date: 2017-07-21
泽塔生物科技(上海)有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

[0003] The drawbacks of these methods are the limited range of taxa that can identify species in one test, poor generalizability, low throughput and high false nega

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  • Molecular identification method and system for eucaryon source components
  • Molecular identification method and system for eucaryon source components

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[0037] The following description with reference to the accompanying drawings is provided to assist in a comprehensive understanding of various embodiments of the present disclosure as defined by the claims and their equivalents. The following description includes various specific details to aid in understanding, but these are to be regarded as merely exemplary. Accordingly, those of ordinary skill in the art will recognize that various changes and modifications of the various embodiments described herein can be made without departing from the scope and spirit of the disclosure. Also, descriptions of well-known functions and constructions may be omitted for clarity and conciseness.

[0038] The terms and words used in the following description and claims are not limited to the bibliographical meanings, but, are merely used by the inventor to enable a clear and consistent understanding of the present disclosure. Accordingly, it should be apparent to those skilled in the art tha...

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Abstract

The invention discloses a molecular identification method and system for eucaryon source components. The method comprises the steps that a whole-genome shotgun method sequence of test material DNA is used as input data to be input; the input data is subjected to feature calculation; a standard sequence pre-stored in a database is used to query input data features, and a comparison result of the input data features and the standard sequence is obtained; and species determination is performed according to the comparison result. The molecular identification method and system are wide in application range and high in universality.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular identification method and system for components derived from eukaryotic organisms. Background technique [0002] Almost all existing molecular biology methods for identifying eukaryotic-derived components are based on specific nucleic acid probes. For example, polymerase chain reaction (PCR) amplification based on specific primers, or the method of specific oligonucleotide probes (biochip). By examining the electrophoresis bands / sequencing results of the amplified products of the PCR reaction, or based on the hybridization signal of the probe, the source of the limited components can be judged. [0003] Drawbacks of these methods are the limited range of taxa that can identify species in one test, poor generalizability, low throughput and high false negative rates (off-target molecular probes). In the face of the situation where the composition of the test m...

Claims

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Application Information

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IPC IPC(8): G06F19/16G06F19/18
CPCG16B15/00G16B20/00
Inventor 王俊宁
Owner 泽塔生物科技(上海)有限公司
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