Biomarkers for cervical cancer.

A cervical marker gene technology, applied in the field of detection of susceptibility to cervical cancer, can solve the problem of low specificity

Active Publication Date: 2017-08-01
UNIVERSITY OF GRONINGEN +1
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  • Abstract
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Problems solved by technology

However, the disadvantage of this technique is that it primarily recognizes large amounts of highly methylated repetitive DNA, resulting in lower specificity

Method used

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  • Biomarkers for cervical cancer.
  • Biomarkers for cervical cancer.
  • Biomarkers for cervical cancer.

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Experimental program
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Embodiment Construction

[0064] Experimental part

[0065] patients and methods

[0066] general strategy

[0067] To characterize the DNA methylation of CIN2 / 3 lesions and identify novel methylation markers of CIN2 or higher (CIN2+), we applied the following strategy (see figure 1 ): First, the MBD of human MECP2 was used to enrich for methylated DNA, and subsequently in two samples from 18 CIN2 / 3 lesions (6 CIN2 and 12 CIN3), 20 normal cervical and healthy volunteer leukocyte DNA. paired-end sequencing (MethylCap-seq) on DNA isolated from fresh-frozen macro-dissected epithelial tissues. To identify differentially methylated regions (DMRs), we retrieved reads from promoter and exonic regions. We selected methylation markers that showed significant differences between normal and CIN2 / 3 cervixes and that the white blood cell count must also be low to prevent false positive results. Markers were graded according to high specificity (unmethylated in normal cervix) and high sensitivity (methylated in...

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Abstract

The invention relates to methods, reagents and kits for detecting the susceptibility to cervical cancer. In particular, it relates to novel methylation markers to improve screening for cervical intraepithelial neoplasia grade 2 / 3(CIN2 / 3) and the use thereof for identifying a cervical cell as neoplastic or predisposed to neoplasia in an isolated sample.

Description

technical field [0001] The present invention relates to methods, reagents and kits for detecting susceptibility to cervical cancer. In particular, the present invention relates to novel methylation markers to improve screening for cervical intraepithelial neoplasia grade 2 / 3, and to novel methylation markers for use in identifying cervical cells in isolated samples as Neoplastic or prone to neoplastic use. Background technique [0002] Cervical cancer is characterized by a definite pre-malignant, cervical intraepithelial neoplasia (CIN). Identification of these CIN lesions by population-based screening programs and their subsequent treatment resulted in a significant reduction in cervical cancer incidence and mortality 1,2 . Pap smear cytology is the most widely used screening method for cervical cancer, but is less than ideal as the detection sensitivity for CIN2 and higher (CIN2+) is only about 55% 3-5 . Cervical cancer is highly correlated with high-risk human papill...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/154C12Q1/708C12Q2600/112
Inventor 海斯贝塔·巴伦蒂娜·阿莉达·维斯曼阿特·赫拉德·扬·范德泽爱德华杜斯·玛丽亚·多米尼克斯·斯胡林
Owner UNIVERSITY OF GRONINGEN
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