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Quality control detection card and detection kit of fluorescent immunochromatography instrument, and preparation method and detection method thereof

A fluorescence immunochromatography and detection card technology, applied in the field of fluorescence immunochromatography instrument quality control detection card, can solve problems such as misdiagnosis, and achieve the effects of simple operation, improved reliability and convenient portability

Inactive Publication Date: 2017-08-11
BIOHIT BIOTECH HEFEI
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Problems solved by technology

[0004] However, due to the factors of the instrument itself and the interference of the external environment, there is a certain probability that the measurement results will cause misdiagnosis.

Method used

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  • Quality control detection card and detection kit of fluorescent immunochromatography instrument, and preparation method and detection method thereof
  • Quality control detection card and detection kit of fluorescent immunochromatography instrument, and preparation method and detection method thereof
  • Quality control detection card and detection kit of fluorescent immunochromatography instrument, and preparation method and detection method thereof

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Embodiment Construction

[0033] In order to better understand the present invention, the present invention will be further described below in conjunction with the examples and accompanying drawings, and the following examples are only to illustrate the present invention rather than limit it.

[0034] like figure 1 As shown, a fluorescent immunochromatography instrument quality control detection card, on the PVC bottom plate 1, a detection window 3 is opened, a nitrocellulose film 2 is fixed in the detection window 3, and a Q C Line 4 and a Q T Line 5, the Q C Line 4 and Q T Line 5 is marked with fluorescent microsphere solution.

[0035] like figure 2 As shown, the preparation method of the fluorescence immunochromatography instrument quality control test card is as follows:

[0036] 1. Solution configuration

[0037] a. Use a pipette to measure 10 μL of BANGS microsphere stock solution with a concentration of 10 mg / mL into a centrifuge tube.

[0038] b. Add 990 μL of PBS diluent to 10 μL of t...

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Abstract

The invention relates to the field of immunochromatographic assay, in particular to a quality control detection card and detection kit of a fluorescent immunochromatography instrument, and a preparation method and detection method thereof. The quality control detection card is characterized in that a detection window is formed in a PVC bottom plate, thin cellulose nitrate film is fixed in the detection window, a QC line and a QT line are arranged on the thin cellulose nitrate film, and both the QC line and the QT line are touched with a fluorescent microsphere solution. At least two quality control detection cards are selected to combine into the quality control detection kit. The QT / QC area ratio of each quality control detection card is calculated in sequence and compared with a standard curve, and when an error is higher than certain value, the measuring result of an instrument is low in reliability. According to the quality control detection method applied to the fluorescent immunochromatography instrument, operation is simple and convenient, and the method can be applied to field detection; instrument quality testing is initially carried out before samples are detected, so that the reliability of sample measuring results is improved.

Description

technical field [0001] The invention relates to the field of immunochromatography detection, in particular to a fluorescence immunochromatography instrument quality control detection card, a detection kit, a preparation method and a detection method thereof. Background technique [0002] Immunochromatography is often used in biological and medical testing. Immunochromatography refers to a unique way of immunoassay. The principle is to immobilize the specific antibody on a certain zone of the nitrocellulose membrane first, and when one end of the dry nitrocellulose is immersed in the sample (urine or serum), the sample solution is made to flow along the membrane through capillary action. When it moves to the area where the receptor (such as antibody or antigen) of the analyte is immobilized, the corresponding analyte in the sample will specifically bind to the receptor. If the immunocolloidal gold Or immunoenzyme staining can make the area show a certain color, so as to ach...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N33/558G01N33/533
CPCG01N33/558G01N33/533G01N33/582
Inventor 张继尧夏果裘桢炜陈迪虎应玉龙洪津
Owner BIOHIT BIOTECH HEFEI
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