Colloidal gold immunochromatography test strip used for detecting human NGAL, kit, and preparation method of colloidal gold immunochromatography test strip and kit

A technology of immunochromatography test paper and colloidal gold, which is applied in the field of kits and its preparation, colloidal gold immunochromatography test strips, and can solve the problems of immunoturbidimetric methodology and clinical application that need to be further verified. Achieve good market application value, accurate and reliable results, and easy storage

CN107045062AActive Publication Date: 2017-08-15RAYBIOTECH INC GUANGZHOU
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2017-08-15

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Abstract

The invention discloses a colloidal gold immunochromatography test strip used for detecting human NGAL, a kit, and a preparation method of the colloidal gold immunochromatography test strip and the kit. The colloidal gold immunochromatography test strip comprises a substrate, and a sample pad, a colloidal gold marked pad, a coating membrane, and water absorption paper which are arranged on the substrate successively; the colloidal gold marked pad is coated with colloidal gold marked anti-human NGAL detection antibody and colloidal gold marked chicken IgY antibody; the coating membrane comprises a detection zone and a reference zone which are arranged in parallel and are separated from each other; the detection zone is coated with anti-human NGAL coating antibody; the reference zone is coated with goat-anti-chicken IgY antibody; the anti-human NGAL detection antibody possesses epitopes corresponding to an amino acid sequence represented by SEQ ID No:5. The colloidal gold immunochromatography test strip is simple, rapid, practical, and sensitive, is high in efficiency, is suitable for clinical rapid detection and on-site rapid detection, is convenient to store, and is suitable to be popularized and applied in primary hospitals, and batch production can be realized.
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Description

technical field

[0001] The invention belongs to the field of biotechnology. Specifically, the invention relates to a colloidal gold immunochromatographic test strip, a test kit and a method for semi-quantitatively detecting neutrophil gelatinase-associated lipocalin (NGAL) in urine. its preparation method. Background technique

[0002] Neutrophil gelatinase-associated lipocalin (neutrophilgelatinase-associated lipocalin, NGAL) was discovered in neutrophils by Kjeldsen et al. in 1993. It is a 25kD glycoprotein. It has a typical β-fold barrel structure of the lipocalin family, and is closely related to inflammation, embryonic development, immune response, chemotaxis, signal transduction, and the occurrence and development of various tumors. NGAL can participate in cell differentiation and inhibit apoptosis as a survival factor; high expression of NGAL can be seen in human tumors such as lung adenocarcinoma, esophageal cancer, pancreatic cancer, colon cancer and breast cancer,...

Examples

Embodiment 1

[0034]Example 1 Colloidal gold immunochromatographic test strip for detecting human NGAL and preparation method thereof

[0035] see figure 1 , is a schematic structural view of the colloidal gold immunochromatographic test strip for detecting human NGAL of the present invention, the colloidal gold immunochromatographic test strip for detecting human NGAL in this embodiment includes a substrate 7, and is arranged on the bottom in turn Sample pad 1, gold mark pad 2, coating film 3 and absorbent paper 6 on lining 7, the chicken IgY antibody that is coated with the anti-human NGAL detection antibody of colloidal gold mark and colloidal gold mark on described gold mark pad 2, so The coating film 3 includes a detection area 4 and a control area 5 arranged in parallel and spaced from each other, the detection area 4 is coated with an anti-human NGAL coating antibody, and the control area 5 is coated with a goat anti-chicken IgY antibody, so The anti-human NGAL detection antibody ha...