Colloidal gold immunochromatography test strip used for detecting human NGAL, kit, and preparation method of colloidal gold immunochromatography test strip and kit

Abstract

The invention discloses a colloidal gold immunochromatography test strip used for detecting human NGAL, a kit, and a preparation method of the colloidal gold immunochromatography test strip and the kit. The colloidal gold immunochromatography test strip comprises a substrate, and a sample pad, a colloidal gold marked pad, a coating membrane, and water absorption paper which are arranged on the substrate successively; the colloidal gold marked pad is coated with colloidal gold marked anti-human NGAL detection antibody and colloidal gold marked chicken IgY antibody; the coating membrane comprises a detection zone and a reference zone which are arranged in parallel and are separated from each other; the detection zone is coated with anti-human NGAL coating antibody; the reference zone is coated with goat-anti-chicken IgY antibody; the anti-human NGAL detection antibody possesses epitopes corresponding to an amino acid sequence represented by SEQ ID No:5. The colloidal gold immunochromatography test strip is simple, rapid, practical, and sensitive, is high in efficiency, is suitable for clinical rapid detection and on-site rapid detection, is convenient to store, and is suitable to be popularized and applied in primary hospitals, and batch production can be realized.

Description

technical field [0001] The invention belongs to the field of biotechnology. Specifically, the invention relates to a colloidal gold immunochromatographic test strip, a test kit and a method for semi-quantitatively detecting neutrophil gelatinase-associated lipocalin (NGAL) in urine. its preparation method. Background technique [0002] Neutrophil gelatinase-associated lipocalin (neutrophilgelatinase-associated lipocalin, NGAL) was discovered in neutrophils by Kjeldsen et al. in 1993. It is a 25kD glycoprotein. It has a typical β-fold barrel structure of the lipocalin family, and is closely related to inflammation, embryonic development, immune response, chemotaxis, signal transduction, and the occurrence and development of various tumors. NGAL can participate in cell differentiation and inhibit apoptosis as a survival factor; high expression of NGAL can be seen in human tumors such as lung adenocarcinoma, esophageal cancer, pancreatic cancer, colon cancer and breast cancer,...

AI Technical Summary

Problems solved by technology

[0009] 3. Transmission immunoturbidimetry——This method is simple, fast, and can be automated, and is suitable for batch detection. However, the methodology and clinical application of immunoturbidimetry still need further verification

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