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Method using Rhodiola rosea liquid-submerged fermentation to produce rhodioloside

A liquid submerged fermentation and salidroside technology, which is applied in the field of Rhodiola submerged fermentation to produce salidroside, can solve the problems of low yield of secondary metabolites, long chemical synthesis route, and low content, and achieve high yield High efficiency, low price, and simple operation

Active Publication Date: 2017-08-18
SHIJIAZHUANG ZANGNUO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical structure and pharmacological effects of the two are clear, but the content in Rhodiola is not high
However, the yield of secondary metabolites of Rhodiola rosea in cell culture is usually low, the chemical synthesis route is long, the yield is low, and the operation is cumbersome, which makes its economic value questionable

Method used

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  • Method using Rhodiola rosea liquid-submerged fermentation to produce rhodioloside

Examples

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Effect test

experiment example 1

[0041] In this experimental example, in the method for producing salidroside by liquid submerged fermentation of Rhodiola rosea, experiments were carried out at different fermentation times to determine a reasonable fermentation time. Except fermentation time, other fermentation conditions etc. all adopt the optimal experiment condition that the present invention proposes. Specifically, this experimental example uses the following steps:

[0042] (1) Get Monascus bacterial classification, be inoculated in the Erlenmeyer flask that liquid seed culture medium is housed (the bacterial classification inoculum amount is 5%), loading capacity 50ml / 250ml, under the temperature of 28 ℃, with the rotating speed of 180r / min Cultivate on a shaker for 14 hours to obtain a seed culture solution; wherein, by weight percentage, the liquid seed culture medium is prepared from the following raw materials: 1.5% peptone, 3% glucose, Mg 2 SO 4 ·3H 2 O0.5%, the balance is water;

[0043] (2) I...

Embodiment 1

[0047] This example provides a method for producing salidroside by liquid submerged fermentation of Rhodiola rosea. Specifically, the method includes the following steps:

[0048] (1) get Monascus bacterial classification, be inoculated in liquid seed culture medium (bacteria classification inoculation amount is 5%), loading capacity 50ml / 250ml, at the temperature of 28 DEG C shaker culture 13 hours, obtain seed culture liquid; Wherein , by weight percentage, the liquid seed medium is prepared from the following raw materials: peptone 1.5%, glucose 3%, Mg 2 SO 4 ·3H 2 O 0.5%, the balance is water;

[0049] (2) Inoculate the seed culture solution prepared in step (1) into 1L of liquid fermentation medium (the inoculum size is to inoculate 50ml of seed culture solution per liter of liquid fermentation medium), and ferment on a shaking table at a temperature of 28°C Cultivate for 48 hours to obtain a fermentation medium; by weight percentage, the liquid fermentation medium is...

Embodiment 2

[0053] This example provides a method for producing salidroside by liquid submerged fermentation of Rhodiola rosea. Specifically, the method includes the following steps:

[0054] (1) get Monascus bacterial classification, be inoculated in liquid seed culture medium (bacteria classification inoculation amount is 5%), loading capacity 50ml / 250ml, shake table culture at 30 ℃ of temperature for 14 hours, obtain seed culture liquid; Wherein , by weight percentage, the liquid seed medium is prepared from the following raw materials: peptone 1.5%, glucose 3%, Mg 2 SO 4 ·3H 2 O 0.05%, the balance is water;

[0055] (2) Inoculate the seed culture solution prepared in step (1) into 1L of liquid fermentation medium (the inoculum size is to inoculate 50ml of seed culture solution per liter of liquid fermentation medium), and ferment on a shaking table at a temperature of 30°C Cultivate for 12 hours to obtain a fermentation medium; by weight percentage, the liquid fermentation medium ...

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Abstract

The invention discloses a method using Rhodiola rosea liquid-submerged fermentation to produce rhodioloside. A liquid fermentation culture medium used by the method is prepared by, by weight percentage, 6-10% of glucose, 2-6% of Rhodiola rosea powder, 0.04-0.15% of MgSO4.3H2O, 0.04-0.15% of CaCl2, 0.2-0.5% of K2HPO4.3H2O, 0.2-0.45% of KH2PO4 and the balance water. The method has the advantages that the yield of the rhodioloside is increased evidently, activated carbon is used to decolor fermentation liquor, the membrane separation technology and the sephadex gel filtration chromatography technology are combined to separate and purify the fermentation liquor, high yield is achieved, and the obtained rhodioloside is high in purity and yield.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for producing salidroside by submerged fermentation of rhodiola rosea liquid. Background technique [0002] Rhodiola (Rhodiola) is a whole herb used as medicine, cold in nature, sweet and astringent in taste. The plant is rich in minerals, has unique chemical composition and high medicinal value, and has been listed as a rare medicinal plant. Rhodiola rosea has the "adaptogen" effect similar to "supporting the root and strengthening the body" of traditional Chinese medicine, anti-cold, anti-microwave radiation, anti-hypoxia, anti-fatigue, and intelligence-building effects, and has excellent properties such as non-toxic and non-addictive. The domestic demand for Rhodiola rosea is gradually increasing, and the annual demand for digging Rhodiola rosea dry rhizomes exceeds 1,000 tons. With the development of science and technology and the improvement of people's livin...

Claims

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Application Information

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IPC IPC(8): C12P19/44C12R1/66
CPCC12P19/44
Inventor 高飞王智森纪玉哲杨红霞
Owner SHIJIAZHUANG ZANGNUO BIOTECH
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