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Apigenin-carrying hyaluronic acid targeted nano assembly and preparation method thereof

A technology of nano-assembly and hyaluronic acid, which is applied in the field of medical drugs, can solve the problems of killing tumor cells, unfavorable effects, and affecting SH targeting ability, so as to increase dissolution rate and dissolution rate, improve dispersion stability, and excellent drug slow release performance

Inactive Publication Date: 2017-08-29
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, Schanté et al. found that the carboxyl group of SH is the recognition site for receptors and enzymes, and the covalently modified carboxyl group will affect the targeting ability of SH, which is not conducive to killing tumor cells

Method used

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  • Apigenin-carrying hyaluronic acid targeted nano assembly and preparation method thereof
  • Apigenin-carrying hyaluronic acid targeted nano assembly and preparation method thereof
  • Apigenin-carrying hyaluronic acid targeted nano assembly and preparation method thereof

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Embodiment

[0025] 1. Obtain the mixed powder of apigenin / sodium hyaluronate by an improved grinding method.

[0026] Weigh 10 mg of apigenin powder and 50 mg of hyaluronic acid powder, mix them evenly, place them in a mortar and grind them thoroughly. Every 6 minutes of grinding, pause for 2 minutes to re-mix, repeat the operation 2 times, and dry in a 70°C oven for 4 minutes. This process reciprocates 3 times, a total of 60 minutes.

[0027] 2. Preparation of apigenin-loaded hyaluronic acid-targeted nanoassemblies

[0028] Weigh 6 mg of the prepared mixed powder and dissolve it in 1.5 ml of distilled water, stir at room temperature for 20 min in the dark at a speed of 300 r / min, to obtain a primary apigenin nanosuspension. Transfer to a 2ml EP tube, and use an ultrasonic cell disruptor for ultrasonication, the ultrasonic conditions are: power 175W, ultrasonic time 5min, ultrasonic work 10s stop 5s. The appearance of the resulting apigenin-loaded hyaluronic acid-targeted nanoassembly c...

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Abstract

The invention discloses an apigenin-carrying hyaluronic acid targeted nano assembly and a preparation method thereof. The system is composed of hydrophilic polysaccharide sodium hyaluronate and a hydrophobic drug namely apigenin. During preparation, firstly, sodium hyaluronate and apigenin powder are fully ground in a grinding bowl; then, the mixed powder is taken to mix with distilled water, and a primary nano suspension is obtained by stirring; and finally, ultrasonic treatment is performed with an ultrasonic cell crusher to obtain the apigenin-carrying hyaluronic acid targeted nano assembly. The drug carrier system disclosed by the invention significantly improves the dissolution rate of a drug and has good physical stability. The other outstanding advantage of the drug carrier system is that the particle size is small, the average particle size is less than 200nm, and passive targeting can be realized by virtue of an EPR (electron paramagnetic resonance) effect of a tumor location. Meanwhile, hyaluronic acid is taken as a carrier, so that the apigenin-carrying hyaluronic acid targeted nano assembly can realize active targeting, reduce toxic and side effects, and improve the treatment efficiency of an antitumor drug.

Description

technical field [0001] The invention relates to the field of medical drugs, in particular to an apigenin-loaded hyaluronic acid targeting nano assembly and a preparation method thereof. Background technique [0002] Apigenin (Apigenin, APG), also known as apigenin, is a dihydroflavonoid compound extracted from the leaves and stems of celery. It has attracted much attention because of its various pharmacological effects and biological properties such as anti-tumor, anti-inflammation, anti-anxiety, lowering blood pressure, and scavenging free radicals, among which the anti-tumor activity is the most significant. Pharmacological studies have shown that apigenin exerts its tumor suppressor effect mainly by inhibiting tumor cell proliferation, invasion and metastasis, inducing tumor cell apoptosis and interfering with tumor cell signaling pathways. Although the drug has shown good development prospects in the field of medicine, its solubility in aqueous solution is extremely poo...

Claims

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Application Information

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IPC IPC(8): A61K47/36A61K31/352A61P35/00
CPCA61K31/352A61K47/36
Inventor 张良珂赵庭
Owner CHONGQING MEDICAL UNIVERSITY
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